Show/hide main menu

Research Laboratories

Basson Laboratory

Principal Investigator: Dr M. Albert Basson

View profile

 

The Basson laboratory studies the function of signalling regulators and chromatin remodelling factors in development in order to uncover the mechanisms underlying congenital disease. The group is primarily interested in neurodevelopmental conditions.

Every specialised cell in the body contains exactly the same genetic information in the form of the genome. Thus, the mechanisms that control cellular differentiation and maintain cellular identity operate at a level “above the genetic code”. These epigenetic mechanisms establish the complement of gene expression that ultimately determines cell fate.

During embryonic development, when cell fates are first determined, cell identity is initially specified in response to signals from the local micro-environment. Many of these signals take the form of secreted growth factors.

The Basson lab has been particularly interested in Receptor Tyrosine Kinase (RTK) signalling. Growth factors like FGFs are used repeatedly in many development contexts and regulate somatic stem cell homeostasis and regeneration in the adult organism.

Our work has demonstrated central roles for Sprouty genes in the regulation of RTK signalling. We have found that removal of these genes can have major impacts on the development of several essential organs, including the kidney, thymus, parathyroid, sensory ganglia, cardiovascular system, midbrain and cerebellum.

Several other organs have been added to this list through the work of a number of other colleagues and independent groups. In addition, in collaboration with Dr. Andrew Brack’s group at Harvard, we have found that Sprouty1 is essential for normal function of muscle stem cells in the adult organism.

 In the News ...
Scientists make old muscles young again in an attempt to combat ageing.
 
 Stem cells need recovery time, too

Current research projects

Development of the cerebellum

A major focus of the laboratory has been to understand how FGF signalling is regulated during development of the mammalian cerebellum. The cerebellum is the part of the brain that is essential for fine motor control and cerebellar defects can result in ataxia.

In addition, the cerebellum has been implicated in cognition and cerebellar hypoplasia is associated with a number of important neurodevelopmental disorders, including autism. By manipulating the levels of FGF signalling during brain development in the mouse, we have identified a specific region of the cerebellum, the vermis, which is particularly sensitive to reductions in FGF signalling. We predict that similar defects in FGF signalling should be associated with vermis hypoplasia in the human population.


cerebellum-autism

Read about the groundbreaking study, The Cerebellum and Autism, which sheds light on the various regulatory mechanisms at play during the development of the mammalian cerebellum, and the relevance of this exciting research to autism and CHARGE syndrome.

International Innovation is the leading global dissemination resource for the wider scientific, technology and research communities, dedicated to disseminating the latest science, research and technological innovations on a global level. More information and a complimentary subscription offer to the publication can be found at: www.researchmedia.eu

 

Chromatin remodelling factors in neural development and autism

Recently, the lab has become interested in members of the CHD chromatin remodeling factors, CHD7 and CHD8. The CHD7 gene is mutated in human CHARGE syndrome, a rare, but devastating syndrome that affects multiple organs.

In collaboration with Pete Scambler’s group at the UCL Institute for Child Health, we have developed mouse models, which allow us to study the developmental causes of brain defects associated with this syndrome. CHD7 primarily associates with distal enhancers where it appears to function as a “rheostat” that fine-tunes the expression levels of developmentally important genes.

We are currently working on identifying the mechanisms whereby the loss of CHD7 result in neurodevelopmental defects

CHD7 is associated with distal gene enhancers, where it interacts with other chromatin remodelling complexes and presumably affects gene expression by remodelling chromatin.

Image: CHD7 is associated with distal gene enhancers, where it interacts with other chromatin remodelling complexes and presumably affects gene expression by remodelling chromatin.

 

 

Mutations in CHD7, and a related factor CHD8, with which it interacts, have been implicated in autism. Recent estimates suggest that autism spectrum disorders affect approximately 1/110 children in the UK and up to 1/88 in the USA.

Our future aims
  • To understand the roles of CHD7 and CHD8 in neural development;
  • To elucidate the mechanisms whereby CHD7 and CHD8 function to fine-tune gene expression;
  • To determine the behavioural consequences of specific cerebellar defects, especially as it relates to autism

Laboratory Members

Research Associate

Dr Graham Kenny

Clinical Research Fellow

Dr Danielle Whittaker


PhD Students

Mr Sahrunizam Kasah

Mr Nemanja Saric

Research Technician

Ms Kimberley Riegman

Contact for further information

Email: albert.basson@kcl.ac.uk

Tel: +44 (0)20 7188 1804

internaladd1
Sitemap Site help Terms and conditions Accessibility Recruitment News Centre Contact us

© 2014 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454