Understanding the 3-dimensional organisation of the molecular and cellular interactions within organ primordia is necessary for a clear understanding of organ formation and function. The Planar Cell Polarity (PCP) pathway is a local signalling system which in epithelia acts along an axis that is perpendicular to apical-basal polarity. My lab aims to dissect PCP signalling function in 3D within the developing vertebrate central nervous system (CNS) with a major focus on PCP signalling activity at distinct cellular interfaces between neuroepithelial precursors. Central to my experimental strategy is the zebrafish CNS. In collaboration with Jon Clarke I will use live imaging of zebrafish CNS development to test how epithelial precursors influence specific aspects of CNS formation, for example neuronal migration, and question how PCP signalling impacts on these processes.