Sites and facilities
Both our Denmark Hill and St Thomas’ sites have access to excellent Clinical Research Facilities (CRFs), which also provide a focus for collaborative programmes with groupings working on developmental programming, nutrition/metabolic syndrome, stroke, stem cell transplantation and sickle cell disease.
We have a broad range of complementary basic and clinical research expertise, including the use of integrated state-of-the-art approaches to the study of cardiovascular biology and disorders. The vast majority of Divisional research laboratories are housed in newly refurbished accommodation and have excellent equipment and facilities. Research methods and techniques in routine use within the Division include:
- State-of-the-art molecular and cell biology and biochemistry, including the application of proteomics and gene transfer.
- Culture models to investigate the biology of endothelial cells, cardiomyocytes, vascular smooth muscle, fibroblasts, and other cells.
- Fluorescence and confocal microscopy, including the use of FRET/FLIM to assess protein-protein interactions; microepifluorescence; and electrophysiology.
- Structural biology, biophysics and bioinformatics.
- Ex vivo and in vivo assessment of cardiac contractile function and haemodynamics including the use of microconductance and pressure-volume analysis both experimentally and in humans.
- Analysis of sarcomere shortening and contractile function in skinned and intact single myocytes.
- Ex vivo and in vivo assessment of vascular function in experimental models and human tissue, using organ baths, tail cuff plethysmography, telemetry, forearm plethysmography, ultrasound, and coronary pressure and flow measurements.
- Defined experimental models of genetic and induced hypertension, cardiac hypertrophy, myocardial infarction, remodelling, heart failure, atherosclerosis, metabolic dysfunction, diabetes, obesity, thrombosis, and inflammation.