Adrian Hayday's research
Adrian Hayday, MA, PhD, F Med Sci
Kay Glendinning Professor
Group Leader, Cancer Research UK
Tel: 44 (0)20 7848 9607
Professor Hayday’s laboratory employs molecular biology approaches to understand how lymphocytes function within tissues, and how those functions may contribute to human health and disease. The laboratory’s basic research includes model systems that permit fundamental questions about immune surveillance to be asked. The molecules and mechanisms identified by those studies are then examined for human counterparts that may teach us about pathogenesis, and provide new tools for application in clinical trials that we undertake. Likewise, we undertake innovative sponsored research agreements relating to the development of novel immunotherapeutics. Although each researcher in the laboratory pursues a clearly defined project, great emphasis is placed on the synergies that can be realised through small teams of researchers working together on the following areas.
1. Lymphoid stress-surveillance: from concept to clinic
2. The Development of Innate-Like T cells
Pierre Vantourout, Vassia Sofra, Rick Wolf, Bodhi Hunt, Stathis Theodoridis
Lymphocytes are traditionally assigned to the antigen-specific adaptive response that takes some time to develop but which can provide long-term memory. However, our work has identified and focussed on compartments of T cells that reside at steady-state within tissues and that respond rapidly to challenges, in synchrony with innate immunity. Our research investigates the functional potential of such “Lymphoid Stress-Surveillance”, and identifies the scenarios in which it is most active. In this regard skin lymphoid stress surveillance appears to be a key regulator of skin graft rejection via a molecule, NKG2D, that is upregulated by tissue dysregulation via mRNA stabilisation. Unexpectedly, NKG2D-dependent T cell activation rapidly provoked lgE syntheiss, thereby establishing a novel link between tissue stress and atropy that we are currently investigating.
3. Lymphoid Stress Surveillance of Cancer
Melanie Wencker, Anett Jandke, Livija Deban, Rosie Hart, Rafael di Marco Barros, Martin Woodward
It has recently become clear from our own, our collaborators’ and others’ work that gamma delta T cells can generate pro-inflammatory effector responses, such as IL-17 and interferon-gamma, far more quickly than can conventional alpha beta T cells. This capacity is laid down during development in the thymus, and it forms a key component of composing a rapid, stress-surveillance response pathway. However, the nature of the molecular engagements in the thymus that may determine this is very poorly understood. To distinguish the roles of some leading candidates that include ligands for the T cell receptor; CD27; and the Lymphotoxin receptor, we are combining the use of thymic organ culture with novel gain-of-function and loss-of-function systems. A major advance in characterising innate-like T cell development was the identification by us and our collaborators of Skint 1, the first genetic determinant of γδ cell development that is required for formation of the murine epidermal T lymphcyte compartment. Skint 1 is expressed exclusively by epithelial cells and its understanding is providing radical new insights into the regulation of T cells by epithelial cells.
4. Immune Responses in Neonates
Fernanda Kyle, Deborah Enting, Marialuisa Iannitto, Yin Wu, Oliver Nussbaumer
There is a surging tide of interest in the prospect of tumour immunotherapy, motivated at least in part by striking efficacies of newly introduced immuno-therapeutics such as ipilimumab. A conspicious group in our understanding is the specificity and properties of tumour-reactive T cells that are activated by such immunotherapies. In particular, we ask whether lymphoid stress-surveillance mechanisms are operative. To this end, primary T cells are harvested from human tumour explants using a novel cyto-protective protocol and their response modes and specificities assessed. The phenotypes and response-modes of human peripheral blood lymphocytes are also assessed in patients in relation to disease and treatment regimens. By the integration of these approaches, we seek to define tumour-reactive T cells; conditions that promote them; and bio-markers for their beneficial effects in patients as they relate to clinical treatment decisions.
5. Generation of an open-source library of mouse knockout immunophenotyping data by the 3i consortium
Whereas the newborn child is highly susceptible to infection, and whereas this susceptibility is further increased in those born prematurely, there is very little description of neonatal immunology. We have undertaken a longitudinal study of T cell biology in prematurely born infants and have identified a novel T cell effector function that contrasts with T cell biology in adults and that conflicts with the view that lymphocytes in newborns are anti-inflammatory, so as to protect developing tissues. The biological and clinical implications of these unexpected findings are under study.
6. Human Immune Response Dynamics (HIRD)
Adam Laing, Dmitry Ushakov, Namita Saran, Keng Hng, Susana Caetano, Lucie Abeler-Dörner
The Infection and Immunity Immunophenotyping (3i) consortium has been formed to conduct a high-throughput immunological phenotyping of approximately 800- 1000 knockout mouse lines generated by the Wellcome Trust Sanger Institute (WTSI) between 2013 and 2018. Funded by the Wellcome Trust and led by King’s College London, the project includes participants from WTSI, Imperial College, and the Universities of Oxford, Cambridge, and Manchester.
The phenotyping has two components, an observational screen and a challenge screen. In the observational screen the immune cell compartments of different lymphoid and non-lyphoid organs are analysed in order to identify genes regulating the immune system at steady state. The challenge screen looks at responses to chemical stress and to viral and bacterial infections that collectively mimic major aspects of human exposure to the environment and that therefore permit the likely identification of genes that regulate the human immune response under challenge. The mechanism of action of such genes is explored in follow-up studies, in our laboratory and in collaboration with the community.
The team at KCL is responsible immunophenotyping the lymphocyte compartments of spleen, lymph nodes and bone marrow by flow cytometry and to analyse the immune compartment in the skin by fluorescent microscopy.
The project makes use of state-of-the-art multicolour flow cytometry and fluorescent microscopy and employs cutting-edge tools for automated data analysis and dissemination. All generated data is open source and available to the scientific community whose involvement will be encouraged by the programme’s outreach component. To learn more, look at results and check the status of your favourite genes, please visit the consortium’s webpage.
Olga Sobolev, Sean O’Farrell
Although the molecules and cells that are key to immune function are well understood, there is little appreciation of how their effects are integrated to human immune responses. To this end we have established the logistical platform to support human immune monitoring and have described the innate and adaptive responses to swine flu vaccination in our model systems, together with project area 5.
- Modi BG, Neustadter J, Binda E, Lewis J, Filler RB, Roberts RB, Roberts SJ, Kwong BY, Reddy S, Overton JD, Galan A, Tigelaar R, Cai L, Fu P, Shlomchik M, KaplanDH, Hayday A. and Girardi M. (2012) "Langerhans Cells Facilitate Epithelial CNA Damage and Squamous Cell Carcinoma" Science 335: 104-108
- Bas A, Swamy M, Abeler-Dörner L, Williams G, Pang DJ, Barbee SD, Hayday A.(2011) "Butyrophilin-like 1 encodes an enterocyte protein that selectively regulates functional interactions with T lymphocytes. Proc Nat. Acad. Sci. USA. 108: 4376-4381.
- Strid, J., Sobolev, O., Zavirova, B., Polic, B., and Hayday A.C. (2011) "The intraepithelial T cell response to NKG2D-ligands links lymphoid stress- surveillance to atopy" Science 334: 1293-1297
- Shafi, S., Vantourout, P., Vaughan, R., Wallace, G.R., Stanford, M., and Hayday A.C. (2011) "An NKG2D-mediated human lymphoid stress-surveillance response with high inter-individual variation." Science Translational Medicine 3 :113ra124.
- Hayday AC. (2009) “Gammadelta T cells and the lymphoid stress-surveillance response”. Immunity 31:184-96.
- Gibbons DL, Haque SF, Silberzahn T, Hamilton K, Langford C, Ellis P, Carr R, Hayday AC. (2009)“Neonates harbour highly active gammadelta T cells with selective impairments in preterm infants”. Eur J Immunol. 39:1794-806.
- Hayday AC, Peakman M. (2008) “The habitual, diverse and surmountable obstacles to human immunology research”. Nat Immunol. 9: 575-80.
- Strid. R., Roberts, S.J., Filler, R.B., Lewis, J.M., Kwong, B.Y., Schpero, W., Kaplan, D.H., Hayday, A. C., Girardi, M. (2008) “Acute upregulation of an NKG2D ligand promotes rapid reorganization of a local immune compartment with pleiotropic effects on carcinogenesis”. Nat Immunol. 9:146-54
- Dieli, F., Vermijlen, D., Fulfaro, F., Caccamo, N., Meraviglia, S., Cicero, G., Roberts, A., Buccheri, S., D’Asaro, M., Gebbia, N., Salerno, A., Eberl, M., Hayday, A. “Targeting human γδ T cells with zoledronate and interleukin-2 for immunotherapy of hormone-refractory prostate cancer”. Cancer Research 67: 7450-7
- Silva-Santos B, Pennington DJ, Hayday AC (2005) “Lymphotoxin-Mediated Regulation of gamma delta Cell Differentiation by alpha beta T Cell Progenitors” Science 307:925-928
- Girardi, M., Oppenheim, D.E., Steele, C.R., Lewis, J., Glusac, E., Filler, R.B., Hobby, P., Sutton, B., Tigelaar, R.E., Hayday, A.C. (2001) “Regulation of Cutaneous Maglinancy by Gamma delta T Cells” Science 294: 605- 609
- Michel ML, Pang DJ, Haque SF, Potocnik AJ, Pennington DJ, Hayday AC. (2012) Interleukin 7 (IL-7) selectively promotes mouse and human IL-17-producing γδ cells. Proc Natl Acad Sci U S A. 109(43):17549-54