Professor Manuel Mayr MD PhD
Professor of Cardiovascular Proteomics
The James Black Centre
King's College, University of London
125 Coldharbour Lane,
London SE5 9NU, UK
Phone: +44 (0) 20 7848 5132
Fax: +44 (0) 20 7848 5296
Biography
Prof Manuel Mayr received his first degree in medicine from the University of Innsbruck, Austria, where he graduated “sub ausspiciis presidentis rei publicae”, the highest distinction awarded for academic education. From 1996-1998 he worked with Prof. Georg Wick at the Institute of Experimental Pathology, Innsbruck, Austria on the role of heat shock proteins in atherosclerosis. Beginning his postdoctoral studies, he joined Prof. Qingbo Xu’s group at the Institute of Biomedical Aging Research of the Austrian Academy of Sciences, working in the area of animal models and cellular signalling in response to biomechanical stress. In 2001, he moved together with Prof. Xu to London. At St. George’s, he developed his proteomic skills and obtained his PhD, entitled “Cardiovascular proteomics: Linking proteomic and metabolomic changes” from the University of London in 2005. In 2006, he spent a sabbatical in Prof. Peipei Ping’s laboratory at the University of California, Los Angeles, to further advance his skills in mass spectrometry. He is now in charge of the new proteomics facility at the James Black Centre that will provide a technology platform for cardiovascular research (www.vascular-proteomics.com).
Prof Mayr is a member of the Editorial Board for Proteomics - Clinical Applications and was recently appointed as Associate Editor for the Journal of Molecular and Cellular Cardiology. He is serving on the American Heart Association Program Committee (Council on Functional Genomics & Translational Biology) and the Management Committees of the British Atherosclerosis Society (BAS), the British Society for Proteome Research (BSPR) and the London Vascular Biology Forum (LVBF).
Research interests
Proteomics and Metabolomics Combined with Genetic Manipulation.
To understand complex biological systems, detailed examination of the properties of their constituent parts is essential, but insufficient. A single gene mutation can cause alterations of seemingly unrelated biochemical pathways. By combining proteomics and metabolomics in animal models of cardiovascular research, we try to bridge the gap between molecular and systems biology.
Stem Cell Differentiation into Vascular Cells.
Stem cell research holds great promise for regenerative medicine and tissue engineering. Our current studies focus to decipher the distinguishing proteomic and metabolic features of stem cell-derived cardiovascular cells. The overall aim is to identify key proteins or small molecules, that may be drug targets for promoting stem cell differentiation or novel paracrine factors leading to arterio- and angiogenesis.
Figure 1: Changes in protein expression of wildtype and apoE-deficient smooth muscle cells as shown by difference in-gel electrophoresis (DIGE).
Figure 2: Mass Spectrometry - Dionex Ultimate 3000 HPLC
Group members
Postdoctoral Fellows
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Dr J Barallobre-Barreiro
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Dr K Dudek
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Ms S Langley
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Dr U Mayr
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Dr P Skroblin
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Dr X Yin
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Dr A Zampetaki
PhD Students
Technicians
Selected publications
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Willeit P, Zampetaki A, Dudek K, Kaudewitz D, King AS, Kirkby NS, Crosby-Nwaobi R, Prokopi M, Drozdov I, Langley S, Sivaprasad S, Markus HS, Mitchell JA, Warner T, Kiechl S and Mayr M. Circulating MicroRNAs as Novel Biomarkers for Platelet Activation. Circ Res. Published online before print January 2, 2013.
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Barallobre-Barreiro J, Didangelos A, Schoendube FA, Drozdov I, Yin X, Fernández-Caggiano M, Willeit P, Puntmann VO, Aldama-López G, Ajay M. Shah AM, Doménech N, Mayr M. Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia- reperfusion injury. Circulation. 2012; 125:789-802. (plus editorial & selected for F1000).
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Zampetaki A, Willeit P, Tilling L, Drozdov I, Prokopi M, Renard JM, Mayr A, Weger, Schett G, Shah A, Boulanger C, Willeit J, Chowienczyk P, Kiechl S, Mayr M. Prospective study on circulating microRNAs and risk of myocardial infarction. J Am Coll Cardiol. 2012; 60:290-299.
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Zampetaki A, Kiechl S, Drozdov I, Willeit P, Mayr U, Prokopi M, Mayr A, Weger S, Oberhollenzer F, Bonora E, Shah A, Willeit J, Mayr M. Plasma microRNA profiling reveals loss of endothelial miR-126 and other microRNAs in type 2 diabetes. Circ Res. 2010; 107:810-817. (Best manuscript award).
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Yin X, Cuello F, Mayr U, Hao Z, Hornshaw M, Ehler E, Avkiran M, Mayr M. Proteomics analysis of the cardiac myofilament subproteome reveals dynamic alterations in phosphatase subunit distribution. Mol Cell Proteomics. 2010; 9:497-509. (plus cover picture).
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Prokopi M, Pula G, Mayr U, Devue C, Gallagher J, Xiao Q, Boulanger CM, Westwood N, Urbich C, Willeit J, Steiner M, Breuss J, Xu Q, Kiechl S, Mayr M. Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures. Blood. 2009;114:723-732.
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Mayr M, Grainger D, Mayr U, Leroyer AS, Leseche G, Sidibe A, Herbin O, Yin X, Gomes A, Madhu B, Griffiths JR, Xu Q, Tedgui A, Boulanger CM. Proteomics, metabolomics, and immunomics on microparticles derived from human atherosclerotic plaques. Circ Cardiovasc Genet. 2009;2:379-388.
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Mayr M, Liem D, Zhang J, Li X, Avliyakulov NK, Yang JI, Young G, Vondriska TM, Ladroue C, Madhu B, Griffiths JR, Gomes A, Xu Q, Ping P. Proteomic and metabolomic analysis of cardioprotection: Interplay between protein kinase C epsilon and delta in regulating glucose metabolism of murine hearts. J Mol Cell Cardiol. 2009;46:268-277.
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Pula G, Mayr U, Evans C, Prokopi M, Vara DS, Yin X, Astroulakis Z, Xiao Q, Hill J, Xu Q, Mayr M. Proteomics identifies thymidine phosphorylase as a key regulator of the angiogenic potential of colony-forming units and endothelial progenitor cell cultures. Circ Res. 2009;104:32-40.
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Mayr M. Metabolomics: ready for the prime time? Circ Cardiovasc Genet. 2008;1:58-65.
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Mayr M, Zampetaki A, Sidibe A, Mayr U, Yin X, De Souza AI, Chung YL, Madhu B, Quax PH, Hu Y, Griffiths JR, Xu Q. Proteomic and metabolomic analysis of smooth muscle cells derived from the arterial media and adventitial progenitors of apolipoprotein E-deficient mice. Circ Res. 2008;102(9):1046-56.
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Mayr M, Yusuf S, Weir G, Chung YL, Mayr U, Yin X, Ladroue C, Madhu B, Roberts N, De Souza A, Fredericks S, Stubbs M, Griffiths JR, Jahangiri M, Xu Q, Camm AJ. Combined metabolomic and proteomic analysis of human atrial fibrillation. J Am Coll Cardiol. 2008;51(5):585-94.
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Mayr M, Basetti M, Xu Q. Proteomics and metabolomics combined in cardiovascular research. Trends Cardiovasc Med. 2007;17(2):43-8.
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Mayr M, Zhang J, Greene AS, Gutterman DD, Perloff JK, Ping P. Proteomic based development of biomarkers in cardiovascular disease: Mechanistic, clinical, and therapeutical insights. Mol Cell Proteomics. 2006;5:1853-64.
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Yin X, Mayr M, Xiao Q, Wang W, Xu Q. Proteomic analysis reveals higher demand for antioxidant protection in embryonic stem cell-derived smooth muscle cells. Proteomics. 2006;6:6437-6446.
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Mayr M, Kiechl S, Tsimikas S, Miller E, Willeit J, Witztum JL, Xu Q. Oxidized low-density lipoprotein antibodies, chronic infections and carotid atherosclerosis in a population-based study. J Am Coll Cardiol. 2006;47:2436-43.
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Mayr M, Chung Y-L, Mayr U, Yin X, Ly L, Hu Y, Griffiths JR, Xu Q. Proteomic and metabolomic analyses of atherosclerotic vessels in ApoE-deficient mice reveal dynamic changes in inflammation, oxidative stress and metabolism. Arterioscl Thromb Vasc Biol. 2005;25:2135-2142.
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Mayr M, Siow R, Chung Y-L, Mayr U, Griffiths JR, Xu Q. A proteomic and metabolomic analysis: Role of protein kinase C delta in vascular smooth muscle cells. Circ Res. 2004;94: e87-96.
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Mayr M, Metzler B, Chung Y-L, Mayr U, McGregor E, Hu Y, Leitges M, Pachinger O, Dunn MJ, Griffiths JR, Xu Q. Ischemic preconditioning exaggerates cardiac damage in PKCdelta null mice. Am J Physiol Heart Circ Phys. 2004;287: H946-956.
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Mayr M, Chung Y-L, Mayr U, McGregor E, Troy H, Hu Y, Leitges M, Dunn MJ, Griffiths JR, Xu Q. Loss of PKCdelta alters cardiac metabolism. Am J Physiol Heart Circ Phys. 2004;287: H937-945.
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Mayr U, Mayr M, Li C, Wernig F, Dietrich H, Hu Y, Xu Q. Loss of p53 accelerates neointimal lesions of vein bypass grafts in mice. Circ Res. 2002; 90:197-204.
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Mayr M, Hu Y, Hainaut P, Xu Q. Mechanical stress-induced oxidative DNA damage and rac-p38 MAPK pathways mediate p53 dependent apoptosis in vascular smooth muscle cells. FASEB J. 2002; 16:1423-1425.
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Leitges M, Mayr M, Braun U, Huemer U, Li C, Pfister G, Ghaffari-Tabrizi N, Baier G, Hu Y, and Xu Q. Excerbated vein graft arteriosclerosis in PKCdelta-null mice. J Clin Invest. 2001; 108:1505-1512.
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Mayr M, Kiechl S, Willeit J, Wick G, and Xu Q. Infections, immunity and atherosclerosis: Associations of antibodies to C. pneumoniae, H. pylori and cytomegalovirus with immune reactions to heat shock protein 60 and atherosclerosis. Circulation. 2000; 102:833-839.
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Mayr M, Li C, Zou Y, Huemer U, Hu Y, and Xu Q. Biomechanical stress-induced apoptosis in vein grafts involves p38 mitogen-activated protein kinases. FASEB J. 2000; 14:261-270.