Members of the Cell Imaging Group share a common interest in developing and applying advanced optical cell imaging techniques to monitor biological processes that involve bioactive molecules in live/fixed cells, cellular organelles, tissues and whole organisms. The biomolecular specificity possible with optical methods has been particularly valuable in microscopy and live cell protein studies. Visualising these biological processes in the context of diseased states may give us new ways of monitoring and interfering with these biological processes.
A main focus in our laboratories is to develop 'pioneering' technologies in the field of Cancer Cell and Tissue Imaging which are aimed to help evaluating individual patients' disease progression in clinic. An additional potential application of these techniques is to identify the subset of patients who are most likely to respond to drugs that target a specific mechanism in cancer cells, initially in the clinical trial setting.
Members of the group are developing imaging techniques for multiphoton imaging in living tissues and high-resolution fluorescence lifetime imaging of cells, and imaging techniques (including 4 pi-imaging, STED, TIRF) to improve imaging resolution, within cells, at depth in biological tissues and at the cell:substrate interface. In addition there are developments in optical biosensors for 'lab-on-a-chip' cellular analysis.
Associated research programmes
Associated staff research interests
The work of the Group involves the development of instrumentation to address specific biological hypotheses, primarily involving imaging and image processing, using automated microscope systems. The particular strengths of the Group are in the application of physics and engineering concepts to design and improve experimental apparatus, from first principles to successful application.
020 7848 6594
The Ameer-Beg group aims to further our understanding of cell signalling dynamics and control. We develop optical instrumentation to address fundamental biological questions regarding the dynamic interaction of protein partners within the cellular membrane. The group’s interests range from high-resolution imaging of tumours using multiphoton fluorescence lifetime imaging to the interrogation of single-molecules within cellular membranes.
Our group is intimately involved in an initiative to develop ‘optical proteomic technology for in situ analysis of protein interaction networks’. This collaboration, involving a number of research groups within the college and led by Professor Malcolm Irving (KCL), aims to develop high-throughput/content optical screening approaches for cell based assays of protein-protein interactions. As part of a strategic programme of research within the biophysics community at KCL, we aim to establish a novel high-throughput fluorescence lifetime imaging (FLIM)/FRET-based assay that identifies intracellular interactions using genome-wide searches, at the protein level, in mammalian cells. To date we have developed a high-content imaging platform for protein interaction screening using steady-state fluorescence anisotropy and fluorescence lifetime imaging readouts.
The Cell Imaging and Biodynamics group is part of the joint UCL/KCL Comprehensive Imaging Centre where we will develop high-resolution multiphoton FLM for measurement of FRET within thick biological specimens.
020 7848 6558
Mathematical biology, analysis of cellular processes, Bayesian analysis of biomedical data
Interplay between signal transduction pathways in control of cell migration using FRET/ FLIM technology.
CONTACTS FOR FURTHER INFORMATION
Professor Tony Ng, Dr Baljinder Mankoo