Dr Philip J Marsh
Print version
BSc Biological Sciences (University of Sussex)
PhD (King's College, University of London)
Lecturer and Academic Director
Molecular Biology Unit
School of Biomedical Sciences
King;'s College London
Member of Diabetes Research Group, Division of Reproduction and Endocrinology.
Telephone: +44 (0)20 7848 6436 / +44 (0)20 7848 6464
Fax: +44 (0)20 7848 6280
E-mail: phil.marsh@kcl.ac.uk
PhD (King's College, University of London)
Lecturer and Academic Director
Molecular Biology Unit
School of Biomedical Sciences
King;'s College London
Member of Diabetes Research Group, Division of Reproduction and Endocrinology.
Telephone: +44 (0)20 7848 6436 / +44 (0)20 7848 6464
Fax: +44 (0)20 7848 6280
E-mail: phil.marsh@kcl.ac.uk
Research Technology
The Molecular Biology Unit (MBU) provides scientific and technical support in DNA technology to research workers in the School of Biomedical Sciences. Core-type services are provided, these being based around machines that the MBU operates centrally and include DNA sequencing, DNA synthesis and real-time PCR for quantitation. An important aspect of the service is the provision of advice and training for workers who lack molecular biology experience and the provision of labspace and project supervision for those from non-molecular biology labs that need to make genetic reagents
Research Interests
Current research interests include
Genetics of pancreas development. Gene expression patterns that lead specifically to pancreas formation in human development are only partially known. There are rare cases of children in which the pancreas fails to develop and we have access (through collaboration with Dr Khalid Hussain at the Institute of Child Health) to families that have multiple apancreatic children. To identify the mutation(s) responsible, we are sequencing a shortlist of several known candidate genes. Homozygosity mapping using single nucleotide polymorphism microarrays will be used to identify unknown candidate genes (link to R Chen) (Peter Jones) (link to Khalid Hussain).
Expression of mammalian membrane proteins in E coli. Many pharmaceutical products are targeted against cell surface proteins that span membranes. Membrane proteins however are difficult to study since they cannot easily be made in preparative quantities using the recombinant methods that have proved so successful for cytosolic proteins. We have developed a novel system enabling large-scale production of human membrane proteins in bacteria (in collaboration with Dr D Barlow, Prof J Lawrence and Dr I Neophytou of the Molecular Biophysics Research Group in in Life Sciences at King’s. The novel system is being exploited by KCL Enterprises
Novel genetic vaccines. Collagen-induced arthritis has been treated in model systems by immunising with immunodominant proteolytic fragments of collagen. We have shown that this protective effect can be partly achieved with a genetic vaccine that expresses intracellular collagen in mice. We have recently made novel genetic fusions that secrete collagen from cells and target it to antigen presenting cells. This is a collaboration with Dr Maqsood Khan, Prof N Staines and Dr S Thompson of King’s College.
Recent Publications
Trivedi N, Marsh P, Wood-Kaczmar A and Gordon-Weeks PR (2004)
Glycogen synthase kinase 3βphosphorylation of microtubule associated protein 1B at serine 1260 contributes to the control of microtubule stability in growing axons
J Cell Sci accepted Dec 2004
Persaud SJ, Roderigo-Milne HM, Squires P, Sugden D, Wheeler-Jones CPD, Marsh PJ, Belin VD,Luther MJ and Jones PM. (2002)
A key role for β-cell cytosolic phospholipase A2 in the maintenance of insulin stores but not in the initiation of insulin secretion. Diabetes 51, 98-104.
Shân L. Gyles, Chris J. Burns, Barbara J. Whitehouse, David Sugden, Phil J. Marsh, Shanta J. Persaud, and Peter M. Jones (2001)
ERKs regulate cyclic AMP-induced steroid synthesis through transcription of the steroidogenic acute regulatory (StAR) gene J. Biol. Chem. 276: 34888-34895
A. T. Linke, B. Marchant, P. Marsh, G. Frampton, J. Murphy, M. L. Rose (2001)
Screening of a HUVEC cDNA library with transplant-associated coronary artery disease sera identifies RPL7 as a candidate autoantigen associated with this disease.
Clin. Exp. Immunol. 126 173-179.
Gould HJ, Mackay GA, Karagiannis SN, O’Toole CM, Marsh PJ and Korngold, R. (1999)
Comparison of IgE and IgG antibody-dependent cytotoxicity in vitro and in a SCID mouse xenograft model of ovarian cancer. Eur. J. Immunol. 29, 3527-3537.
The Molecular Biology Unit (MBU) provides scientific and technical support in DNA technology to research workers in the School of Biomedical Sciences. Core-type services are provided, these being based around machines that the MBU operates centrally and include DNA sequencing, DNA synthesis and real-time PCR for quantitation. An important aspect of the service is the provision of advice and training for workers who lack molecular biology experience and the provision of labspace and project supervision for those from non-molecular biology labs that need to make genetic reagents
Research Interests
Current research interests include
Genetics of pancreas development. Gene expression patterns that lead specifically to pancreas formation in human development are only partially known. There are rare cases of children in which the pancreas fails to develop and we have access (through collaboration with Dr Khalid Hussain at the Institute of Child Health) to families that have multiple apancreatic children. To identify the mutation(s) responsible, we are sequencing a shortlist of several known candidate genes. Homozygosity mapping using single nucleotide polymorphism microarrays will be used to identify unknown candidate genes (link to R Chen) (Peter Jones) (link to Khalid Hussain).
Expression of mammalian membrane proteins in E coli. Many pharmaceutical products are targeted against cell surface proteins that span membranes. Membrane proteins however are difficult to study since they cannot easily be made in preparative quantities using the recombinant methods that have proved so successful for cytosolic proteins. We have developed a novel system enabling large-scale production of human membrane proteins in bacteria (in collaboration with Dr D Barlow, Prof J Lawrence and Dr I Neophytou of the Molecular Biophysics Research Group in in Life Sciences at King’s. The novel system is being exploited by KCL Enterprises
Novel genetic vaccines. Collagen-induced arthritis has been treated in model systems by immunising with immunodominant proteolytic fragments of collagen. We have shown that this protective effect can be partly achieved with a genetic vaccine that expresses intracellular collagen in mice. We have recently made novel genetic fusions that secrete collagen from cells and target it to antigen presenting cells. This is a collaboration with Dr Maqsood Khan, Prof N Staines and Dr S Thompson of King’s College.
Recent Publications
Trivedi N, Marsh P, Wood-Kaczmar A and Gordon-Weeks PR (2004)
Glycogen synthase kinase 3βphosphorylation of microtubule associated protein 1B at serine 1260 contributes to the control of microtubule stability in growing axons
J Cell Sci accepted Dec 2004
Persaud SJ, Roderigo-Milne HM, Squires P, Sugden D, Wheeler-Jones CPD, Marsh PJ, Belin VD,Luther MJ and Jones PM. (2002)
A key role for β-cell cytosolic phospholipase A2 in the maintenance of insulin stores but not in the initiation of insulin secretion. Diabetes 51, 98-104.
Shân L. Gyles, Chris J. Burns, Barbara J. Whitehouse, David Sugden, Phil J. Marsh, Shanta J. Persaud, and Peter M. Jones (2001)
ERKs regulate cyclic AMP-induced steroid synthesis through transcription of the steroidogenic acute regulatory (StAR) gene J. Biol. Chem. 276: 34888-34895
A. T. Linke, B. Marchant, P. Marsh, G. Frampton, J. Murphy, M. L. Rose (2001)
Screening of a HUVEC cDNA library with transplant-associated coronary artery disease sera identifies RPL7 as a candidate autoantigen associated with this disease.
Clin. Exp. Immunol. 126 173-179.
Gould HJ, Mackay GA, Karagiannis SN, O’Toole CM, Marsh PJ and Korngold, R. (1999)
Comparison of IgE and IgG antibody-dependent cytotoxicity in vitro and in a SCID mouse xenograft model of ovarian cancer. Eur. J. Immunol. 29, 3527-3537.