Mass spectrometry is a very sensitive technique that can detect analytes at very low concentrations. Contamination of preparations with keratin and plastisizer can be a problem as they can mask your protein of interest with their greater abundance.
To get the best possible LC/MS/MS analysis, we employ the following protocols. For further information and advice on sample preparation, please contact us and we will be happy to help.
Procedure for staining 1D gels with MS compatible Colloidal Coomassie
Coomassie Staining (PDF, 191 kb)
Procedure for Silver Staining using a Modified Plus One staining kit
Silver Staining (PDF, 194 kb)
In-gel Digestion Protocol for Proteins Separated by PAGE for MS analysis
In-Gel Digestion (PDF, 198 kb)
In-solution Digestion of Complex Biological Matrices
Complex Sample Digestion (PDF, 200 kb)