Protein quantitation
Labeling protein digests with isobaric tags can allow many different biological samples to be mixed together in one analysis. This can enable simultaneous identification of proteins for relative quantitation across the set of samples.
Low molecular weight reporter ions from the mass tags are generated by fragmentation in the mass spectrometer and their intensity is measured, the ratios of which determine the relative amount of protein in each sample. The approach is now being widely used to compare control cases with different disease states in one experiment for example.
If you are thinking about undertaking any of the following techniques then please contact the Facility and we can discuss your needs and help in the experimental design of your project.
Tandem Mass Tag Isobaric Tagging
The Facility offers protein quantitation utilsing TMT® reagents in TMTduplex, TMTsixplex, TMT10plex and TMT11plex.
Each tag contains three regions, namely, a mass reporter region, a cleavable linker region for mass normalization and a protein reactive group.
The chemical structures of the tags are identical but contain isotopes substituted at different positions making the mass reporter and mass normalization regions that differ by the number of isotopic substitutions.
For more detailed information on the TMT tags you can follow the link here