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Facilities

Haematology core equipment

Cell Sorting Facility

Flow Cytometry Facility
The core Flow cytometry facility headed by Professor Eric So in Haemato-oncology at the Rayne Institute (Denmark Hill) can provide a service for multicolour analyses and cell sorting. We have a BD LSR II analyzer (3 lasers and 17 parameters configuration) and a BD FACS Aria cell sorter (Custom order Hi-end sorter with 5 lasers and 17 parameters) suitable for use in a wide range of research and applications.

The configurations for these machines are as follows:

BD LSR II

  • 3 lasers: Blue (488nm), Violet (405nm) and Red (633nm).
  • 17 Parameters / 15 Colours
  • Low, medium and high acquisition controls with fine tuning option
  • Full inter and intra-laser compensation matrix
  • Simultaneous measurement of height, area and width of all parameters

BDLKSRII425w

BD LSR II
The current charge for using the LSR II is £30/hour including the operator cost.


BD FACS Aria

  • 5 lasers: Blue (488nm), Yellow-green (560nm), Red (640nm), Violet (405nm) and Ultraviolet (355nm)
  • 17 parameters / 15 colours
  • 2 nozzle sizes: 70μm and 100μm to accommodate a range of cells and particles
  • Aerosol management system for containment of droplets incorporating biological hazard
  • Temperature control system for maintaining the sorted cells at required temperature (4-42oC)
  • 2 or 4 way or sorting into plates (6, 12, 24 or 96 well plates)
     

BDFACS425w

BD FACS Aria
The equipment in this core facility is available to use only by properly trained and approved individuals. We also provide services to people from other departments in the college as well as external users.


The charges for Aria are as follows:

For KCL Haematology, Neuroscience and Cardiology users who have contributed to the purchase cost of the machine:£100/hr for the first hour and £60/hr for subsequent hours (including operator charge)


For all other (External) users:
£120/hr for the first hour and £80/hr for subsequent hours (including operator charge)


If you wish to discuss and book a slot for sorting/analysis, please contact Dr Tsz Kan Fung.

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