Expertise and Facilities
BHF Centre PIs benefit from state-of-the-art facilities and foci of excellence associated with the 3 main sections involved in the Centre:
School of Cardiovascular Medicine & Sciences
Comprehensive core cardiovascular biomedicine facilities include cell and molecular biology; complex biochemical assays; cellular electrophysiology and microepifluorescence; confocal and time-lapse microscopy; a viral vector core; in vitro physiology; histology; murine microsurgery; in vivo murine phenotyping; clinical investigation and a dedicated clinical vascular function laboratory. There is a dedicated mass spectrometry facility for proteomics and metabolomics.
Randall Division of Cell and Molecular Biophysics
Facilities include single-molecule force-spectroscopy; protein purification and crystallization; site-directed protein labeling; muscle fibre reconstitution; fluorescence polarisation microscopy; muscle X-ray diffraction analysis. The Centre for Biomolecular Spectroscopy has outstanding expertise and equipment for protein-protein interaction analysis by NMR spectroscopy, surface plasmon resonance, isothermal titration calorimetry and optical spectroscopy. The Nikon Imaging Centre provides access to the very latest advanced cell/tissue imaging approaches, e.g. FRET, FLAP, GFP-complementation; spinning disk / laser / multiphoton / STED / STORM super-resolution microscopy; intravital microscopy.
School of Biomedical Engineering and Imaging sciences
There are comprehensive facilities for pre-clinical and clinical in vivo cardiovascular imaging, including dedicated research PET/CT, SPECT/CT, MRI (1.5, 3, 7T; XMR; PET/MR), multinuclear and hyperpolarized spectroscopy; embedded chemistry for design/synthesis of new probes; physics/ maths/ computer science for advances in image acquisition, processing and co-registration.
The Centre for Medical Engineering at King's provides substantial biomedical engineering and computational modeling expertise.