The Ameer-Beg Group
The Ameer-Beg group aims to further our understanding of cell signalling dynamics and control. We develop optical instrumentation to address fundamental biological questions regarding the dynamic interaction of protein partners within the cellular membrane. The group’s interests range from high-resolution imaging of tumours using multiphoton fluorescence lifetime imaging to the interrogation of single-molecules within cellular membranes.
Our group is intimately involved in an initiative to develop ‘optical proteomic technology for in situ analysis of protein interaction networks’. This collaboration, involving a number of research groups within the college and led by Professor Malcolm Irving, aims to develop high-throughput/content optical screening approaches for cell based assays of protein-protein interactions.
As part of a strategic programme of research within the biophysics community at King's, we aim to establish a novel high-throughput fluorescence lifetime imaging (FLIM)/FRET-based assay that identifies intracellular interactions using genome-wide searches, at the protein level, in mammalian cells. To date we have developed a high-content imaging platform for protein interaction screening using steady-state fluorescence anisotropy and fluorescence lifetime imaging readouts.
The Cell Imaging and Biodynamics group is part of the joint King's & UCL Comprehensive Imaging Centre where we will develop high-resolution multiphoton FLM for measurement of FRET within thick biological specimens.