Dr Dylan Owen
Tel: +44 (0) 20 7848 6852/7448
Education and training
MSci Physics, 1st Class (Hons), Imperial College London (2000-2004)
MRes Chemical Biology (Distinction), Imperial College London (2004-2005)
PhD Biomedical Optics. Imperial College London (2005-2008)
Post doctoral research fellow, University of New South Wales (2008-2012)
Lecturer, King’s College London (2013-)
My research interests are in the development and application of cutting-edge fluorescence microscopy techniques and their applications to cell biology, in particular the study of cell membranes and T cells. The microscope techniques particularly include super-resolution fluorescence imaging based on single-molecule detection as well as associated data analysis. I also work with environmentally-sensitive fluorescent probes with spectrally-resolved detection as well as fluorescence lifetime imaging. These are applied to cell biology applications. I am particularly interested in the structure and function of the cell membrane and how this regulates cellular signalling events. An especially important application of this is the study of the T cell immunological synapse between the immune cell and its target cell during an immune response.
Conformational states of the kinase Lck regulate clustering in early T cell signaling. J. Rossy, D.M. Owen, D.J. Williamson, Z. Yang and K. Gaus. Nature Immunology, 14 82-89 (2013).
Sub-resolution lipid domains exist in the plasma membrane and regulate protein diffusion and distribution. D.M. Owen, D.J. Williamson, A. Magenau and K. Gaus. Nature Communications 3, 1256 (2012).
Lipid and lyso-lipid effects on the mechanosensitivity of MscL and MscS co-reconstituted into liposomes. T. Nomura, C.G. Cranfield, E. Deplazes, D.M. Owen, A. Macmillan, A.R. Battle, M. Sokabe and B. Martinac. Proceedings of the National Academy of Sciences (PNAS) 109(22), 8770-8775 (2012).
Imaging membrane order using Laurdan and di-4-ANEPPDHQ in live cells and whole organisms. D.M. Owen, C. Rentero, A. Abu-Siniyeh, A. Magenau and K. Gaus. Nature Protocols 7(1), 24-35 (2012).
Pre-existing LAT clusters do not participate in early T cell signaling events. D.J. Williamson*, D.M. Owen*, J. Rossy*, A. Magenau, M. Wehrmann, J.J. Gooding and K. Gaus. Nature Immunology 12(7), 655-662 (2011). *Equal first author
Primary human CD4+ T cells have diverse levels of membrane lipid order that correlate with their function. L. Miguel, D.M. Owen, C. Leibig, C. Lim, J. Evans, A.I. Magee and E.C. Jury. Journal of Immunology 186(6), 3505-3516 (2011).
Dynamics of sub-synaptic vesicles and surface microclusters at the immunological synapse. M.A. Purbhoo, H.B. Liu, S. Oddos, D.M. Owen, M.A.A. Neil, S.V. Pageon, P.M.W. French, C.E. Rudd and D.M. Davis. Science Signaling 3(121), ra36 1-10 (2010).