Show/hide main menu

Sun Group

Methods and protocols

Fluorescence for in situ structure (FISS)

Fluorescence for in situ structure (FISS) has been developed to measure the orientation of protein domains. In this technique a target protein domain is expressed in a modified form containing a suitable pair of cysteines, covalently modified with a bifunctional rhodamine, then reintroduced to its native environment. The orientation of the rhodamine dipole, and hence of the vector joining the cysteines, is measured in situ, on the millisecond time-scale, using fluorescence polarisation. The protein domain orientation is determined by multiple probes on the target domain. Performing FISS experiment involves a unique combination of expertise -- from molecular biology, protein biochemistry, muscle physiology to biophysics of data interpretation.

SunGrp_Fig2_Methods(420x274)

Sitemap Site help Terms and conditions  Privacy policy  Accessibility  Modern slavery statement  Contact us

© 2020 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454