Stem Cell Research
The PI's involved in this area of research within the Division of Women's Health are Professor Peter Braude and Dr Dusko Ilic.
Clinical grade human embryonic stem cell (hESC) lines
Despite important recent advances in the generation of hESC lines in the effort to generate lines that might be suitable for therapeutic use, there is still some way to go in the development of reliable and efficient methods to allow advancement towards hESC for clinical use, and in the directed differentiation of hESC down particular lineages.
A number of groups world-wide have reported hESC derivation using a variety of conditions, but few have reported derivation under conditions that would normally be regarded as suitable for human use. Within the last 12 months we brought up our derivation technique to clinical grade acceptable level and using this approach we derived successfully under defined xeno-free conditions nine hESC lines. Two normal lines were derived from frozen embryos, whereas seven disease-specific lines were derived from fresh post-preimplantation genetic diagnosis (PGD) embryos.
Disease-specific hESC lines
hESC lines derived from human embryos with genetic mutations following PGD can provide an inexhaustible source of cells that can be differentiated into many cell types and used to further understand the molecular basis of serious diseases for which PGD has been undertaken. Guy’s has the largest and most successful PGD unit in the UK, through which we have privileged access to embryos affected by a range of genetic disorders, including Huntington disease, spinal muscular atrophy, Fragile-X syndrome, and a range of sex linked disorders such as Duchenne muscular dystrophy. Three out of seven disease-specific lines derived within the last 12 months carry little characterized mutation in Von Hippel-Lindau gene and, at our knowledge, are unique in the world.
King's hES cell lines catalogue
Please see the King's hES cell lines catalogue.
For more information and availability contact:
Dr Dusko Ilic
- Stephenson EL, Braude PR (2010) Derivation of the King's College London human embryonic stem cell lines. In Vitro Cell Dev Biol Anim. 94, 1529, e11-4. [PubMed]
- Stephenson E, Ogilvie CM, Patel H, Cornwell G, Jacquet L, Kadeva N, Braude P, Ilic D (2010) Safety paradigm: genetic evaluation of therapeutic grade human embryonic stem cells. J R Soc Interface. 6;7 Suppl 6:S677-88. [PubMed]
- Ilic D, Stephenson E, Wood V, Jacquet L, Stevenson D, Petrova A, et al. Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions. Cytotherapy. 2011 Oct 27. [Epub ahead of print] [PubMed]
- Murdoch A, Braude P, Courtney A, Brison D, Hunt C, Lawford-Davies J, Moore H, Stacey G, Sethe S; for the Procurement Working Group of the National Clinical hESC Forum. The Procurement of Cells for the Derivation of Human Embryonic Stem Cell Lines for Therapeutic Use: Recommendations for Good Practice. Stem Cell Rev. 2011 Jun 14. [Epub ahead of print] [PubMed]