Speaker Dr Sergio Padilla-Parra, Reader in Virus Imaging and Biophotonics, Department of Infectious Diseases and Randall Centre for Cell & Molecular Biophysics, KCL

Title Employing light microscopy to understand HIV-1 entry

Host Fiona Wardle

 

Abstract In our lab we employ Quantitative light microscopy (QLM), which we believe plays a pivotal role in unravelling the intricate mechanisms underlying HIV-1 entry into host cells. By employing QLM techniques, we label both the Gag and Env proteins, preserving their functionality, thus providing a dynamic visualization of the viral entry process. This methodology enables the tracking of individual viral particles in real-time, allowing for the characterization of their interactions with host cell receptors and intracellular trafficking pathways. However, a significant challenge lies in understanding how the dynamics of the Env protein modulate the entry process, a question that remains largely unanswered. Furthermore, the application of correlative light and electron microscopy (CLEM) is paramount in elucidating the maturation process of HIV-1, offering insights into the structural alterations occurring during viral assembly and budding. By integrating QLM and CLEM approaches, we aim to achieve a comprehensive understanding of HIV-1 entry and maturation, thus paving the way for the development of novel therapeutic strategies targeting these critical stages of the viral life cycle.