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Speaker Dr Sergio Padilla-Parra, Reader in Virus Imaging and Biophotonics, Department of Infectious Diseases and Randall Centre for Cell & Molecular Biophysics, KCL

Title Employing light microscopy to understand HIV-1 entry

Host Fiona Wardle


Abstract In our lab we employ Quantitative light microscopy (QLM), which we believe plays a pivotal role in unravelling the intricate mechanisms underlying HIV-1 entry into host cells. By employing QLM techniques, we label both the Gag and Env proteins, preserving their functionality, thus providing a dynamic visualization of the viral entry process. This methodology enables the tracking of individual viral particles in real-time, allowing for the characterization of their interactions with host cell receptors and intracellular trafficking pathways. However, a significant challenge lies in understanding how the dynamics of the Env protein modulate the entry process, a question that remains largely unanswered. Furthermore, the application of correlative light and electron microscopy (CLEM) is paramount in elucidating the maturation process of HIV-1, offering insights into the structural alterations occurring during viral assembly and budding. By integrating QLM and CLEM approaches, we aim to achieve a comprehensive understanding of HIV-1 entry and maturation, thus paving the way for the development of novel therapeutic strategies targeting these critical stages of the viral life cycle.

Event details

New Hunt’s House
Great Maze Pond, London, SE1 9RT