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Multiscale and multiphase organization of the transcriptome during development and in response to the environment

Speaker: Dr Arnaud Hubstenberger, Institut de Biologie Valrose, Nice

Host:  Snezhana Oliferenko

Abstract: Gene networks must be coordinated with organism development and adapted to the environment. We and others introduced phase separation as a framework to study networks of nucleic acids and proteins. Using human cell lines and C elegans germline as models, we previously showed how RNA molecules can exist under different material states (1-3). RNA can be soluble and diffuse or can condense into liquid droplets, semi-liquid hydrogels, or solidify into amorphous aggregates or crystal-like solids. Diverse types of condensates coexist within the cell, and each of these condensates is further comprised of a heterogeneous mix of sub-compartments. Here we show, how the multiscale and multiphase organization of the transcriptome is regulated during development and responds to a physiological range of environmental variations. Analyzing the transcriptomics and proteomics of our FAPS purification of RNA condensates, we dissected networks of structural interactions that separates RNAs into various condensates such as P-bodies and stress granules. We propose a model in which RNA phase separations coordinate the expression of RNA regulons to cellular activity.

 

Event details

Classroom G8, New Hunt's House
Guy’s Campus
Great Maze Pond, London SE1 1UL