Reprogramming Cell Fate

Title: Reprogramming Cell Fate

Guest Speaker: Constantinos Chronis PhD, Assistant Professor, University of Illinois at Chicago, Biochemistry & Molecular Genetics, Chicago

Professor Chronis will be visiting King's. Kostas has been a Post-Doctoral Fellow in Kathrin Plath's Lab at UCLA and is about to open his own lab at the University of Illinois at Chicago. 

Host: Paul Lavender and Shaun Thomas

Recent publications:

Constantinos Chronis cellular reprogramming papers:

The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation.

*Zhang W, *Chronis C, Chen X, Zhang H, Spalinskas R, Pardo M, Chen L, Wu G, Zhu Z, Yu Y, Yu L, Choudhary J, Nichols J, Parast MM, Greber B, Sahlén P, Plath K. 

Cell Stem Cell. 2019 Jan 3;24(1):138-152.e8. doi: 10.1016/j.stem.2018.12.001.

* equal contribution

Comparison of reprogramming factor targets reveals both species-specific and conserved mechanisms in early iPSC reprogramming.

Fu K, Chronis C, Soufi A, Bonora G, Edwards M, Smale ST, Zaret KS, Plath K, Pellegrini M.

BMC Genomics. 2018 Dec 22;19(1):956. doi: 10.1186/s12864-018-5326-1.

Promoter-Enhancer Communication Occurs Primarily within Insulated Neighborhoods.

Sun F, Chronis C, Kronenberg M, Chen XF, Su T, Lay FD, Plath K, Kurdistani SK, Carey MF.

Mol Cell. 2019 Jan 17;73(2):250-263.e5. doi: 10.1016/j.molcel.2018.10.039.

Loss of MECP2 Leads to Activation of P53 and Neuronal Senescence.

Ohashi M, Korsakova E, Allen D, Lee P, Fu K, Vargas BS, Cinkornpumin J, Salas C, Park JC, Germanguz I, Langerman J, Chronis C, Kuoy E, Tran S, Xiao X, Pellegrini M, Plath K, Lowry WE.

Stem Cell Reports.2018 May 8;10(5):1453-1463. doi: 10.1016/j.stemcr.2018.04.001.

X Chromosome Dosage Influences DNA Methylation Dynamics during Reprogramming to Mouse iPSCs.

Pasque V, Karnik R, Chronis C, Petrella P, Langerman J, Bonora G, Song J, Vanheer L, Sadhu Dimashkie A, Meissner A, Plath K.

Stem Cell Reports. 2018 May 8;10(5):1537-1550. doi: 10.1016/j.stemcr.2018.03.019.

Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.

Xue Y, Pradhan SK, Sun F, Chronis C, Tran N, Su T, Van C, Vashisht A, Wohlschlegel J, Peterson CL, Timmers HTM, Kurdistani SK, Carey MF.

Mol Cell. 2017Aug 17;67(4):594-607.e4. doi: 10.1016/j.molcel.2017.06.029. 

Cooperative Binding of Transcription Factors Orchestrates Reprogramming.

*Chronis C, *Fiziev P, Papp B, Butz S, Bonora G, Sabri S, Ernst J, Plath K.

Cell. 2017 Jan 26;168(3):442-459.e20. doi: 10.1016/j.cell.2016.12.016. * equal contribution 

See also: DNA deep dive reveals ways to increase iPSC efficiency. CIRM blog, stories that caught our eye https://blog.cirm.ca.gov/2017/01/20/stories-that-caught-our-eye-20-5-m-in-new-cirm-discovery-awards-sickle-cell-disease-cell-bank-ipsc-insights/

and

Study pinpoints how skin cells’ identity can be disassembled to create stem cells. UCLA News, January 19, 2017 http://newsroom.ucla.edu/releases/ucla-study-pinpoints-how-skin-cells-identity-can-be-disassembled-to-create-stem-cells

Human Naive Pluripotent Stem Cells Model X Chromosome Dampening and X Inactivation.

Sahakyan A, Kim R, Chronis C, Sabri S, Bonora G, Theunissen TW, Kuoy E, Langerman J, Clark AT, Jaenisch R, Plath K.

Cell Stem Cell. 2017 Jan 5;20(1):87-101. doi: 10.1016/j.stem.2016.10.006. 

Human Embryonic Stem Cells Do Not Change Their X Inactivation Status during Differentiation.

Patel S, Bonora G, Sahakyan A, Kim R, Chronis C, Langerman J, Fitz-Gibbon S, Rubbi L, Skelton RJP, Ardehali R, Pellegrini M, Lowry WE, Clark AT, Plath K.

Cell Rep. 2017 Jan 3;18(1):54-67. doi: 10.1016/j.celrep.2016.11.054. 

Long-range chromatin contacts in embryonic stem cells reveal a role for pluripotency factors and polycomb proteins in genome organization.

Denholtz M, Bonora G, Chronis C, Splinter E, de Laat W, Ernst J, Pellegrini M, Plath K.

Cell Stem Cell. 2013 Nov 7;13(5):602-16. doi: 10.1016/j.stem.2013.08.013.

 

Proteomic and genomic approaches reveal critical functions of H3K9 methylation and heterochromatin protein-1γ in reprogramming to pluripotency.

Sridharan R, *Gonzales-Cope M, *Chronis C, Bonora G, McKee R, Huang C, Patel S, Lopez D, Mishra N, Pellegrini M, Carey M, Garcia BA, Plath K.

Nat Cell Biol. 2013 Jul;15(7):872-82. doi: 10.1038/ncb2768. * equal contribution