Dr Jakub Nedbal
Jakub Nedbal obtained amaster's in Physics from Masaryk University in Brno, Czech Republic, and master's in Immunology from King’s College London. During his PhD at King’s, he developed tools to image class switch recombination in human primary B cells genome by means of fluorescence in-situ hybridization. He then moved onto the development of techniques for fluorescence lifetime imaging and flow cytometry. He developed the first fluorescence lifetime flow cytometer based on the light efficient detection method of time-correlated single-photon counting (TCSPC). He co-developed a fast TCSPC microscope based on a new parallelized single-photon avalanche diode array detector.
Jakub is currently working on the development of new fluorescence lifetime-based cell imaging techniques involving spatially resolved TCSPC detectors. These include fluorescence lifetime imaging of thick samples, such as whole organisms, and low phototoxicity fluorescence lifetime imaging in cells. Beyond academic roles, Jakub has worked in commercial R&D in several companies. Up until 2018, he was the Product Development Lead for a startup company commercialising one of the first semiconductor TCSPC array cameras.
- Fluorescence lifetime
- Microscope development
- SPAD Arrays