Booth Group

Paula Booth's group works at the interface of Chemistry and Biology to elucidate the mechanisms by which proteins fold, assemble and communicate within biological membranes. Emphasis is placed on understanding the role of membrane lipids in regulating protein folding and function. Membrane proteins account for about 30% of all cell proteins, and play pivotal roles in life reflected in their domination of drug targets. The group’s research aims to not only solve fundamental questions relating to membrane protein biogenesis and folding, but also to exploit the self-assembly of artificial biomembranes for Synthetic Biology and catalytic applications.

Members

How lipids affect the energetics of co-translational alpha helical membrane protein folding
Brady, R., Harris, N. J., Pellowe, G. A., Breen, S. G. & Booth, P. J., 28 Feb 2022, (E-pub ahead of print) In: Biochemical Society Transactions. 50, 1, p. 555-567 13 p. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1042/BST20201063
Cell-Free Expression to Probe Co-Translational Insertion of an Alpha Helical Membrane Protein
Blackholly, L. R., Harris, N. J., Findlay, H. E. & Booth, P. J., 2 Feb 2022, In: Frontiers in Molecular Biosciences. 9, 795212. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.3389/fmolb.2022.795212
Cell-Free Synthesis Strategies to Probe Co-translational Folding of Proteins Within Lipid Membranes
Harris, N. J., Reading, E. & Booth, P. J., 2022, Methods in Molecular Biology. Humana Press Inc, p. 273-292 20 p. (Methods in Molecular Biology; vol. 2433). Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review. DOIs: https://doi.org/10.1007/978-1-0716-1998-8_17
The membrane transporter lactose permease increases lipid bilayer bending rigidity
Lopez Mora, N., Findlay, H. E., Brooks, N. J., Purushothaman, S., Ces, O. & Booth, P. J., 7 Sep 2021, In: Biophysical Journal. 120, 17, p. 3787-3794 8 p. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1016/j.bpj.2021.06.038
Integrating Membrane Transporter Proteins into Droplet Interface Bilayers
Findlay, H. E., Harris, N. J. & Booth, P. J., 2021, Methods in Molecular Biology. Humana Press Inc, p. 31-41 11 p. (Methods in Molecular Biology; vol. 2315). Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review. DOIs: https://doi.org/10.1007/978-1-0716-1468-6_2
Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry
Strutt, R., Hindley, J. W., Gregg, J., Booth, P. J., Harling, J. D., Law, R. V., Friddin, M. S. & Ces, O., 14 Feb 2021, In: Chemical Science. 12, 6, p. 2138-2145 8 p. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1039/d0sc03889j
Detergent-free purification and reconstitution of functional human serotonin transporter (SERT) using diisobutylene maleic acid (DIBMA) copolymer
Dilworth, M. V., Findlay, H. E. & Booth, P. J., 1 Jul 2021, In: Biochimica et Biophysica Acta - Biomembranes. 1863, 7, 183602. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1016/j.bbamem.2021.183602
Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter
Jia, R., Martens, C., Shekhar, M., Pant, S., Pellowe, G., Lau, A. M., Findlay, H., Harris, N., Tajkhorshid, E., Booth, P. & Politis, A., 2 Dec 2020, In: Nature Communications. 11, 6162. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1038/s41467-020-20032-3
Perturbed structural dynamics underlie inhibition and altered efflux of the multidrug resistance pump AcrB
Reading, E., Ahdash, Z., Fais, C., Ricci, V., Kan, X. W., Grimsey, E., Stone, J., Malloci, G., Lau, A., Findlay, H., Konijnenberg, A., Booth, P., Ruggerone, P., Vargiu, A. V., Piddock, L. JV. & Politis, A., 1 Dec 2020, In: Nature Communications. 11, 1, 5565. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1038/s41467-020-19397-2
Capturing membrane protein ribosome nascent-chain complexes in a native-like environment for co-translational studies
Pellowe, G. A., Findlay, H. E., Lee, K., Gemeinhardt, T. M., Blackholly, L. R., Reading, E. & Booth, P. J., 4 Aug 2020, In: Biochemistry. 59, 30, p. 2764-2775 12 p. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1021/acs.biochem.0c00423

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Group leads

Paula Booth

Head of Department of Chemistry

Booth Group

Members

Laura Blackholly

Paula Booth

Ryan Brady

Heather Findlay

Nicola Harris

How lipids affect the energetics of co-translational alpha helical membrane protein folding

Cell-Free Expression to Probe Co-Translational Insertion of an Alpha Helical Membrane Protein

Cell-Free Synthesis Strategies to Probe Co-translational Folding of Proteins Within Lipid Membranes

The membrane transporter lactose permease increases lipid bilayer bending rigidity

Integrating Membrane Transporter Proteins into Droplet Interface Bilayers

Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry

Detergent-free purification and reconstitution of functional human serotonin transporter (SERT) using diisobutylene maleic acid (DIBMA) copolymer

Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter

Perturbed structural dynamics underlie inhibition and altered efflux of the multidrug resistance pump AcrB

Capturing membrane protein ribosome nascent-chain complexes in a native-like environment for co-translational studies

Group leads

Paula Booth

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