Cell Phenotyping Group

The group launched within the framework of the Human Induced Pluripotent Stem Cells Initiative (HipSci) project, funded by Wellcome and MRC implementing methods to characterise cell behaviour of human iPS cells based on microscopy images, collating data with genomics. This work has formed the basis for an innovative collaborative cell phenotyping space - The Stem Cell Hotel.

The research group led by Davide focuses in particular on two model systems: human iPS and patient-derived brain tumour cells. The combination of imaging, biology and artificial microenvironments is aimed directly towards establishing novel platforms for translational research with applications to drug discovery, precision medicine and future therapeutic approaches.

Davide holds an MD from University of Milan, a PhD in Molecular Oncology from the European Institute of Oncology and completed his postdoctoral training with Austin Smith and Steve Pollard at University of Cambridge and University College London. He is a FLIER (Future Leader in Innovation, Enterprise and Research). of the Academy of Medical Sciences.

Members

Davide Danovi

Senior Lecturer

Defining cell behaviour of human iPS cells using high content imaging

This work started within the framework of the Human induced pluripotent stem cells intiative (HipSci). More recently, phd student Haneen Alsehli has been studying iPS forming sheroids using high content analysis and artificial microenvironments to define inter-relationships between morphology of these structures and lineage differentiation. Haneen Alsehli – Human iPS form spheroids in self-renewing (left) and differentiating (right) conditions (blue, nuclei – red, phalloidin – gray, 3D reconstruction)

Defining cell behaviour of patient-derived brain tumour cells using high content imaging

Wellcome PhD student Victoria Tsang and Research Associate Jason Coombes are exploring the cell behaviour of patient-derived brain tumour cells using artificial microenvironments and high content analysis. Victoria Tsang – Human iPS-derived neural spheroid (brightfield) with protruding axons cocultured with invasive patient-derived glioblastoma cells (purple).

Human iPSC-derived hepatocyte system models cholestasis with tight junction protein 2 deficiency
Li, C. Z., Ogawa, H., Ng, S. S., Chen, X., Kishimoto, E., Sakabe, K., Fukami, A., Hu, Y. C., Mayhew, C. N., Hellmann, J., Miethke, A., Tasnova, N. L., Blackford, S. J. I., Tang, Z. M., Syanda, A. M., Ma, L., Xiao, F., Sambrotta, M., Tavabie, O., Soares, F. & 6 others, Baker, O., Danovi, D., Hayashi, H., Thompson, R. J., Rashid, S. T. & Asai, A., Apr 2022, In: JHEP Reports. 4, 4, 100446. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1016/j.jhepr.2022.100446
High content Image Analysis to study phenotypic heterogeneity in endothelial cell monolayers
Chesnais, F., Hue, J., Roy, E., Branco, M., Stokes, R., Pellon, A., Le Caillec, J., Elbahtety, E., Battilocchi, M., Danovi, D. & Veschini, L., 15 Jan 2022, In: Journal of Cell Science. 135, 2, 259104. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1242/jcs.259104
Plating human iPSC lines on micropatterned substrates reveals role for ITGB1 nsSNV in endoderm formation
Vickers, A., Tewary, M., Laddach, A., Poletti, M., Salameti, V., Fraternali, F., Danovi, D. & Watt, F. M., 9 Nov 2021, In: Stem cell reports. 16, 11, p. 2628-2641 14 p. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1016/j.stemcr.2021.09.017
High-Content Imaging to Phenotype Human Primary and iPSC-Derived Cells
Veschini, L., Sailem, H., Malani, D., Pietiäinen, V., Stojiljkovic, A., Wiseman, E. & Danovi, D., 2021, In: Methods in molecular biology (Clifton, N.J.). 2185, p. 423-445 23 p. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1007/978-1-0716-0810-4_27
ILC1 drive intestinal epithelial and matrix remodelling
Jowett, G. M., Norman, M. D. A., Yu, T. T. L., Rosell Arévalo, P., Hoogland, D., Lust, S. T., Read, E., Hamrud, E., Walters, N. J., Niazi, U., Chung, M. W. H., Marciano, D., Omer, O. S., Zabinski, T., Danovi, D., Lord, G. M., Hilborn, J., Evans, N. D., Dreiss, C. A., Bozec, L. & 5 others, Oommen, O. P., Lorenz, C. D., da Silva, R. M. P., Neves, J. F. & Gentleman, E., 28 Feb 2021, In: NATURE MATERIALS. 20, 2, p. 250-259 10 p. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1038/s41563-020-0783-8
CONTAIN: An open-source shipping container laboratory optimised for automated COVID-19 diagnostics
Danovi, D., 20 May 2020. Research output: Working paper/Preprint › Preprint. DOIs: https://doi.org/, https://www.biorxiv.org/content/10.1101/2020.05.20.106625v1
An integrated pipeline for high-throughput screening and profiling of spheroids using simple live image analysis of frame to frame variations
Alsehli, H., Mosis, F., Thompson, C., Hamrud, E., Wiseman, E., Gentleman, E. & Danovi, D., 21 May 2020, In: Methods. 0, p. 1 Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1016/j.ymeth.2020.05.017
Cardelino: computational integration of somatic clonal substructure and single-cell transcriptomes
HipSci Consortium, 1 Apr 2020, In: NATURE METHODS. 17, 4, p. 414-421 8 p. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1038/s41592-020-0766-3
Amplification of Mdmx (or Mdm4) Directly Contributes to Tumor Formation by Inhibiting p53 Tumor Suppressor Activity (vol 24, pg 5835, 2004)
Danovi, D., Meulmeester, E., Pasini, D., Migliorini, D., Capra, M., Frenk, R., de Graaf, P., Francoz, S., Gasparini, P., Gobbi, A., Helin, K., Pelicci, P. G., Jochemsen, A. G. & Marine, J-C., Aug 2019, In: Molecular and Cellular Biology. 39, 15 Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1128/MCB.00150-19
High-throughput micropatterning platform reveals Nodal-dependent bisection of peri-gastrulation-associated versus preneurulation-associated fate patterning
Tewary, M., Dziedzicka, D., Ostblom, J., Prochazka, L., Shakiba, N., Heydari, T., Aguilar-Hidalgo, D., Woodford, C., Piccinini, E., Becerra-Alonso, D., Vickers, A., Louis, B., Rahman, N., Danovi, D., Geens, M., Watt, F. M. & Zandstra, P. W., Oct 2019, In: PLoS Biology. 17, 10, e3000081. Research output: Contribution to journal › Article › peer-review. DOIs: https://doi.org/10.1371/journal.pbio.3000081

View all publications

Davide was awarded the FLIER (Future Leader for Innovation Enterprise and Research) from the Academy of Medical Sciences.

Grants:

Project	Awarded by:	Awarded to:	Value:	Dates:	Reason:
A Proof of Concept study for the reprogramming of iPSC to manufacture cancer killing neutrophils	MedCity Ltd	Danovi, D.	£99,999.90	1/01/2020 → 31/12/2020	Research
Use of 'Axon-like' Nanofibres to quantify and guide Glioblastoma cells migration	Rosetrees Trust	Danovi, D.	£60,000.00	1/09/2019 → 31/08/2021	Research
Using iPSC variation to define HIV-1 regulatory networks	MRC Medical Research Council	Malim, M., Vigilante, A., Danovi, D., Apolonia, L.	£1,631,019.00	1/08/2019 → 31/07/2024	Research
Impact of missense mutations in recessive Mendelian disease: insight from ciliopathies	Wellcome Trust	Danovi, D., Watt, F.	£206,112.00	1/10/2018 → 30/09/2023	Research

London Stem Cell Network - Annual

19th October 2021 – The Francis Crick Institute (and online)

London Stem Cell Network - Annual

Cytodata 2021

Online 13-14-15 October 2021

Cytodata 2021

Ms Haneen Alsehli

Ms Victoria Tsang

Mr Sam Acors (with Mike Malim)

Mr Matthew Tegtmeier (with Fiona Watt)

Our partners

GlamAI - https://www.glamorous.ai partner in Innovate UK funded project ‘Glioblastoma Multiforme Patient Stratification through Novel integration of Artificial Intelligence, Big Data and Phenotypic Screening’)

LIfT BioSciences - https://www.liftbiosciences.com partner in MedCity funded project ‘A Proof of Concept study for the reprogramming of iPSC to manufacture cancer killing neutrophils’

In parallel to his role at King's, Davide has recently joined an award-winning Cambridge biotech.

GlamAI

LIfT BioSciences

Group lead

Dr Davide Danovi

Senior Lecturer

Contact us

davide.danovi@kcl.ac.uk

Tweets by davide_danovi

Cell Phenotyping Group

Members

Davide Danovi

Defining cell behaviour of human iPS cells using high content imaging

Defining cell behaviour of patient-derived brain tumour cells using high content imaging

Human iPSC-derived hepatocyte system models cholestasis with tight junction protein 2 deficiency

High content Image Analysis to study phenotypic heterogeneity in endothelial cell monolayers

Plating human iPSC lines on micropatterned substrates reveals role for ITGB1 nsSNV in endoderm formation

High-Content Imaging to Phenotype Human Primary and iPSC-Derived Cells

ILC1 drive intestinal epithelial and matrix remodelling

CONTAIN: An open-source shipping container laboratory optimised for automated COVID-19 diagnostics

An integrated pipeline for high-throughput screening and profiling of spheroids using simple live image analysis of frame to frame variations

Cardelino: computational integration of somatic clonal substructure and single-cell transcriptomes

Amplification of Mdmx (or Mdm4) Directly Contributes to Tumor Formation by Inhibiting p53 Tumor Suppressor Activity (vol 24, pg 5835, 2004)

High-throughput micropatterning platform reveals Nodal-dependent bisection of peri-gastrulation-associated versus preneurulation-associated fate patterning

London Stem Cell Network - Annual

Cytodata 2021

Our partners

Group lead

Dr Davide Danovi

Contact us

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