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The Integrated Biological Imaging Network aims to bring together expertise from across the UK to advance the field of biological imaging.

Our Scientific Goals

The IBIN has three scientific goals that are key issues in cell biology. They are to: define the spatiotemporal dynamics of cell adhesion signalling; determine cell-specific cues that influence immune cell-tissue interactions; determine how tissue mechanics influence cell growth and signalling. Details of these goals can be seen on the themes tab below.

These broad goals have been further refined to a set of challenges that the IBIN wishes to achieve through research project funding. These challenges can be found on the 'Activities' tab below and the five research areas that we are currently focusing on can be found on our funding resources page, on the 'Awards' tab below.

Sign Up

To access funding for small projects and sabbaticals, and to be informed of the latest developments from the network then sign up here.

Registration is free and you will receive invitations to future events, workshops and calls for funding applications.

Themes

iSIM imaging of cell adhesion structures
Define the spatiotemporal dynamics of cell adhesion signalling

The process of molecular activation and unfolding events during cell-matrix adhesion complex formation is still not fully understood. Furthermore, how these signalling pathways are modulated by the extracellular environment is still to be fully elucidated. It is the aim of the IBIN to develop 3D models and advanced microscopy techniques to further our understanding of these processes.

    3D spheroids forming in suspended drops
    Determine cell-specific cues that influence immune cell-tissue interactions

    IBIN aims to develop probes that enable the determination of how altered signals from cells/tissue influence immune cell migration and interactions. Cancer cells in 3D ECM and monolayers of lung epithelial cells on 3D scaffolds will be co-cultured with immune cells. Advanced imaging techniques will be used to determine the interactions between these cells/tissues and shed light on the interplay between the immune system and cancer.

      A cell embedded in a collagen matrix
      Determine how tissue mechanics influence cell growth and signalling

      Define how the ECM controls cell behaviour over a timescale of minutes to hours. This will be via ECM organisation, mechano-sensitivity and cell phenotypes. 3D models, such as scaffolds of tuneable stiffness and mouse models will be used to identify the gene activation and signalling pathways regulated by the ECM. Optogenetics and imaging techniques such as photoactivation will enable the identification of the role of ECM in cell growth and signalling.

        Image missing an alt value
        Integrative Biological Imaging Network (IBIN)

        The Integrated Biological Imaging Network aimed to bring together expertise from across the UK to advance the field of biological imaging

        Awards

        Funding Resources

        The Integrated Biological Imaging Network is funding four kinds of applications:

        – Pump-Prime: 1-3 months, £11k max

        This is for 1-3 month-long projects that are exploring a novel biological imaging problem. The Network will fund a post-doc position for up to three months as well as consumables to develop a project to a point where further funding applications can be made.
        Download form here.

        – Pump-Prime: 3-6 months, £25k max

        A small number of more in-depth projects will be funded up to £25k if they are closely aligned with the challenges outlined below.
        Download form here.

        – Sabbaticals (COVID-dependant): 1-4 weeks, £5k max

        To encourage collaboration and enabling IBIN members to develop their skills, these sabbaticals are for travel to another lab for up to one month to enable the development of a project. This could be to gain access to a piece of equipment that you require or to teach another lab a skill that you have.
        Download form here.

        – Short Trip (COVID-dependant): 1-2 days

        This funding is for one or two-day trips that will aid networking and project development.
        Download form here.

        If you have any questions please get in touch with IBIN@kcl.ac.uk


        Funding Allocation Stipulations:

        • Please have a quick read of this document before applying.
        • We are governed by MRC funding guidelines and you can read about those here.
        • Check out the bioimaging challenges described below to determine if your project falls within one or more of the areas identified.

        Funding Criteria Checklist:

        Please compare your application to this scoring sheet. Your application will be processed based on these scoring criteria.

        Public Engagement Fund: up to £500

        We can fund bioimaging public engagement projects worth up to £500. Get in touch with maddy.parsons@kcl.ac.uk for more information.


        Challenges

        At the January 2019 meeting, a number of bioimaging challenges were identified. These challenges can be viewed on this page and have led to five areas that we would like research proposals to focus on:

        1. Image cells in a population in 3D culture/in vivo (~300um thick) at highest possible spatial and temporal resolution. Monitor cell shape, movement and division for ≥24h with minimal phototoxicity. Develop new software to define/reduce background noise, compensate for/track movement from 3D time-lapse datasets and define relationships between cells.
        2. Track multiple (≥4) probes/colours in cells in 3D cultures (in vitro/in vivo) using spectral unmixing/detection over long time periods with minimal phototoxicity.
        3. Develop methods to precisely photo-activate/trigger optogenetic probes in 3D cultures and follow a longer-term readout on cell behaviour/phenotypic changes.
        4. Super-resolution analysis of cells in 3D environments; trial different approaches for live and fixed samples and provide benchmarking of resolution, probe and speed limitations.
        5. Image mechanical changes in/between cells and nuclear morphology in 3D cultures/tissues. Extract mechanical and morphological changes and computationally correlate.

        When applying for IBIN funding, please take into consideration these five challenge areas.


        FAQs

        Can I apply for multiple projects?

        • The named researcher can only apply for one round of Sabbatical and one Pump-Prime funding per academic year. A researcher may be named as a co-researcher on multiple applications, but only if those projects contain different groups of researchers and the projects are significantly different.

        Does the funding cover PI costs such as estates and indirect costs?

        • The funding can be used to cover estates and indirect costs.

        How flexible is the funding?

        • The maximum available funding for Pump-Priming is £25k split between consumables and salary. There is flexibility with how you use these funds i.e. using some consumables to cover salary but must not exceed £25k.

        When should projects start?

        • Projects should commence within two months of funding allocation.

        What’s the difference between Directly Allocated and Directly Incurred?

        • ‘Directly Allocated’ covers the costs researchers working on a project where the costs are an estimate. Where costs are actual, auditable and verifiable, they should be included under the Directly Incurred heading.

        Who should be listed as an investigator or researcher?

        • The person carrying out the bulk of the hands-on work should be named as the ‘researcher’. Anyone who provides expertise or guidance to the researcher should be listed as an investigator.

        If I apply for £25k, would I only receive £20k from IBIN at 80%fEC?

        • The maximum amount that IBIN will fund is £25k. Applications can be higher to reflect the 80% fEC i.e. to receive £25k you could apply for £31.25k and your institution is expected to cover the 20%.

        Activities

        Bio needs 3D
        Challenge areas identified from January 2019 IBIN meeting

        – Computational – Probe Development – Super-Resolution – 3D Bioimaging Instrumentation – 3D Biomechanics Each of these challenges is described in more detail below. Please use these as a guide for developing projects for IBIN funding.

        blog-g0b2278a3a_1280
        iBIN - our BLOG series

        A series of short articles written by the iBIN team highlighting the work undertaken by the project and some of the challenges they have had to overcome.

        Network Team

        Prof Maddy Parsons

        Network Director Cell Biology

        Maddy’s research team aim to understand how membrane receptors control signalling events leading to cell adhesion and migration. They focus on applying advanced microscopy methods to analyse dynamic changes in these processes in 2D and 3D model systems both in healthy and diseased cells.


         

        Dr Simon Ameer-Beg

        Co-Director Functional Imaging


        Prof Gail McConnell

        Network Partner Tissue technology

        Gail McConnell is Chair of Biophotonics at the Department of Physics, University of Strathclyde, UK. The work in Gail’s group involves the design and development of new biomedical imaging technologies spanning the nanoscale to the whole organism.


        Prof Clemens Kaminski

        Network Partner Sensor technology

        Professor of Chemical Physics at the University of Cambridge. The Laser Analytics Group develops and applies advanced optical imaging techniques to unravel molecular mechanisms in live cell and organism models of disease. He is director of the EPSRC CDT in Sensor Technologies and Applications, co-founder of the Cambridge Advanced Imaging Centre, and a fellow of the Optical Society of America. 


         

        Dr Christopher Dunsby

        Network Partner Dynamic Imaging


         

        Prof Nick Brown

        Network Partner Tissue Biology


         

        Prof Michelle Peckham

        Network Partner Super-Resolution


         

        Dr Cristina lo Celso

        Network Partner Stem + Immunology


        Prof Andrew Beeby

        Network Partner Probe development

        As Professor in the Department of Chemistry at Durham University, Beeby applies optical spectroscopy to the study of new materials and is an expert in luminescent materials. Beeby researches the photophysical and photochemical behaviour of condensed-phase molecules and systems with potential commercial importance. He develops new methodologies and designs and constructs sophisticated instrumentation and associated data analysis.

        Themes

        iSIM imaging of cell adhesion structures
        Define the spatiotemporal dynamics of cell adhesion signalling

        The process of molecular activation and unfolding events during cell-matrix adhesion complex formation is still not fully understood. Furthermore, how these signalling pathways are modulated by the extracellular environment is still to be fully elucidated. It is the aim of the IBIN to develop 3D models and advanced microscopy techniques to further our understanding of these processes.

          3D spheroids forming in suspended drops
          Determine cell-specific cues that influence immune cell-tissue interactions

          IBIN aims to develop probes that enable the determination of how altered signals from cells/tissue influence immune cell migration and interactions. Cancer cells in 3D ECM and monolayers of lung epithelial cells on 3D scaffolds will be co-cultured with immune cells. Advanced imaging techniques will be used to determine the interactions between these cells/tissues and shed light on the interplay between the immune system and cancer.

            A cell embedded in a collagen matrix
            Determine how tissue mechanics influence cell growth and signalling

            Define how the ECM controls cell behaviour over a timescale of minutes to hours. This will be via ECM organisation, mechano-sensitivity and cell phenotypes. 3D models, such as scaffolds of tuneable stiffness and mouse models will be used to identify the gene activation and signalling pathways regulated by the ECM. Optogenetics and imaging techniques such as photoactivation will enable the identification of the role of ECM in cell growth and signalling.

              Image missing an alt value
              Integrative Biological Imaging Network (IBIN)

              The Integrated Biological Imaging Network aimed to bring together expertise from across the UK to advance the field of biological imaging

              Awards

              Funding Resources

              The Integrated Biological Imaging Network is funding four kinds of applications:

              – Pump-Prime: 1-3 months, £11k max

              This is for 1-3 month-long projects that are exploring a novel biological imaging problem. The Network will fund a post-doc position for up to three months as well as consumables to develop a project to a point where further funding applications can be made.
              Download form here.

              – Pump-Prime: 3-6 months, £25k max

              A small number of more in-depth projects will be funded up to £25k if they are closely aligned with the challenges outlined below.
              Download form here.

              – Sabbaticals (COVID-dependant): 1-4 weeks, £5k max

              To encourage collaboration and enabling IBIN members to develop their skills, these sabbaticals are for travel to another lab for up to one month to enable the development of a project. This could be to gain access to a piece of equipment that you require or to teach another lab a skill that you have.
              Download form here.

              – Short Trip (COVID-dependant): 1-2 days

              This funding is for one or two-day trips that will aid networking and project development.
              Download form here.

              If you have any questions please get in touch with IBIN@kcl.ac.uk


              Funding Allocation Stipulations:

              • Please have a quick read of this document before applying.
              • We are governed by MRC funding guidelines and you can read about those here.
              • Check out the bioimaging challenges described below to determine if your project falls within one or more of the areas identified.

              Funding Criteria Checklist:

              Please compare your application to this scoring sheet. Your application will be processed based on these scoring criteria.

              Public Engagement Fund: up to £500

              We can fund bioimaging public engagement projects worth up to £500. Get in touch with maddy.parsons@kcl.ac.uk for more information.


              Challenges

              At the January 2019 meeting, a number of bioimaging challenges were identified. These challenges can be viewed on this page and have led to five areas that we would like research proposals to focus on:

              1. Image cells in a population in 3D culture/in vivo (~300um thick) at highest possible spatial and temporal resolution. Monitor cell shape, movement and division for ≥24h with minimal phototoxicity. Develop new software to define/reduce background noise, compensate for/track movement from 3D time-lapse datasets and define relationships between cells.
              2. Track multiple (≥4) probes/colours in cells in 3D cultures (in vitro/in vivo) using spectral unmixing/detection over long time periods with minimal phototoxicity.
              3. Develop methods to precisely photo-activate/trigger optogenetic probes in 3D cultures and follow a longer-term readout on cell behaviour/phenotypic changes.
              4. Super-resolution analysis of cells in 3D environments; trial different approaches for live and fixed samples and provide benchmarking of resolution, probe and speed limitations.
              5. Image mechanical changes in/between cells and nuclear morphology in 3D cultures/tissues. Extract mechanical and morphological changes and computationally correlate.

              When applying for IBIN funding, please take into consideration these five challenge areas.


              FAQs

              Can I apply for multiple projects?

              • The named researcher can only apply for one round of Sabbatical and one Pump-Prime funding per academic year. A researcher may be named as a co-researcher on multiple applications, but only if those projects contain different groups of researchers and the projects are significantly different.

              Does the funding cover PI costs such as estates and indirect costs?

              • The funding can be used to cover estates and indirect costs.

              How flexible is the funding?

              • The maximum available funding for Pump-Priming is £25k split between consumables and salary. There is flexibility with how you use these funds i.e. using some consumables to cover salary but must not exceed £25k.

              When should projects start?

              • Projects should commence within two months of funding allocation.

              What’s the difference between Directly Allocated and Directly Incurred?

              • ‘Directly Allocated’ covers the costs researchers working on a project where the costs are an estimate. Where costs are actual, auditable and verifiable, they should be included under the Directly Incurred heading.

              Who should be listed as an investigator or researcher?

              • The person carrying out the bulk of the hands-on work should be named as the ‘researcher’. Anyone who provides expertise or guidance to the researcher should be listed as an investigator.

              If I apply for £25k, would I only receive £20k from IBIN at 80%fEC?

              • The maximum amount that IBIN will fund is £25k. Applications can be higher to reflect the 80% fEC i.e. to receive £25k you could apply for £31.25k and your institution is expected to cover the 20%.

              Activities

              Bio needs 3D
              Challenge areas identified from January 2019 IBIN meeting

              – Computational – Probe Development – Super-Resolution – 3D Bioimaging Instrumentation – 3D Biomechanics Each of these challenges is described in more detail below. Please use these as a guide for developing projects for IBIN funding.

              blog-g0b2278a3a_1280
              iBIN - our BLOG series

              A series of short articles written by the iBIN team highlighting the work undertaken by the project and some of the challenges they have had to overcome.

              Network Team

              Prof Maddy Parsons

              Network Director Cell Biology

              Maddy’s research team aim to understand how membrane receptors control signalling events leading to cell adhesion and migration. They focus on applying advanced microscopy methods to analyse dynamic changes in these processes in 2D and 3D model systems both in healthy and diseased cells.


               

              Dr Simon Ameer-Beg

              Co-Director Functional Imaging


              Prof Gail McConnell

              Network Partner Tissue technology

              Gail McConnell is Chair of Biophotonics at the Department of Physics, University of Strathclyde, UK. The work in Gail’s group involves the design and development of new biomedical imaging technologies spanning the nanoscale to the whole organism.


              Prof Clemens Kaminski

              Network Partner Sensor technology

              Professor of Chemical Physics at the University of Cambridge. The Laser Analytics Group develops and applies advanced optical imaging techniques to unravel molecular mechanisms in live cell and organism models of disease. He is director of the EPSRC CDT in Sensor Technologies and Applications, co-founder of the Cambridge Advanced Imaging Centre, and a fellow of the Optical Society of America. 


               

              Dr Christopher Dunsby

              Network Partner Dynamic Imaging


               

              Prof Nick Brown

              Network Partner Tissue Biology


               

              Prof Michelle Peckham

              Network Partner Super-Resolution


               

              Dr Cristina lo Celso

              Network Partner Stem + Immunology


              Prof Andrew Beeby

              Network Partner Probe development

              As Professor in the Department of Chemistry at Durham University, Beeby applies optical spectroscopy to the study of new materials and is an expert in luminescent materials. Beeby researches the photophysical and photochemical behaviour of condensed-phase molecules and systems with potential commercial importance. He develops new methodologies and designs and constructs sophisticated instrumentation and associated data analysis.

              Our Partners

              King's College London

              King's College London

              Biotechnology and Biological Sciences Research Council

              Biotechnology and Biological Sciences Research Council

              The Engineering and Physical Sciences Research Council

              The Engineering and Physical Sciences Research Council (EPSRC)

              UKRI 780x440

              UK Research and Innovation (UKRI)

              Leica Microsystems logo

              Leica Microsystems

              Group lead

              Contact us

              New Hunts House,
              Guy's Campus,
              King's College London,
              London SE1 1UL