The Abbelight SAFe MN360 enables super-resolution imaging by detecting and localising individual fluorescent molecules with nanometre precision. Fluorophores are driven to a dark state and activated in sparse subsets so that each appears as an isolated spot, allowing its position to be determined accurately. By accumulating these localisations over thousands of frames, a reconstructed image with resolution well below the diffraction limit is generated.

A key feature is simultaneous multicolour imaging via spectral unmixing, enabling accurate co-localisation of different molecular species without sequential acquisition. Combined with flexible illumination modes (e.g. TIRF, HILO, EPI) and integrated analysis software, the system provides a streamlined workflow for quantitative nanoscale imaging of biological samples.

The MN360 enhances this approach through uniform illumination (ASTER), which improves localisation accuracy across the entire field of view, making results more quantitative and reproducible. It also supports 3D imaging using engineered point spread functions and dual-camera detection, allowing precise localisation in all three spatial dimensions.

To achieve the fluoresce behaviours that enable SMLM, samples need to be prepared in specific way to allow the blinking “behaviour”. Please reach out to us if you are interested in this imaging technique so that we can discuss the needed preparation steps.

Objectives

100x 1.49 NA TIRF (Oil)

100x 1.35 NA (Silicone)

60x 1.42 NA (Oil)

60x 1.3 NA (Silicone)

25x 1.05 NA (Silicone)

20x 0.8 NA (Air)

4x 0.2 NA (Air)

Nikon AXR

Excitation wavelengths

405 nm

488 nm

560 nm

640 nm

Detectors

1 x PMT-MA Multi Alkali Unit

3 x PMT-GA GaAsP Unit

Abbelight SAFe MN360

Excitation wavelengths

- 405 nm

- 488 nm

- 560 nm

- 640 nm