Identifying molecular regulators of muscle regeneration in ageing and disease Muscle is an essential tissue needed for locomotion, eating and regulation of metabolism. In ageing, cancer and degenerative diseases, muscle wasting occurs, likely due to changes to muscle stem cells (muSCs) that prevent them from repairing muscle. We use zebrafish as a model organism for understanding how muscle is maintained or repaired. We aim to identify molecules that regulate muSCs and achieve this by combining live imaging of fluorescently labelled cells with manipulation of gene function and measures of gene function. We are currently focusing on how chromatin organisation is related to muSC behaviour and the genes that are regulated by chromatin modifying factors. We are also examining how muSC function is affected in Telomerase mutants in which there is premature ageing. By identifying molecular and cellular changes in muSCs of Telomerase mutants we hope to understand why muscle weakness occurs during ageing.