Proteasome-catalysed peptide splicing
Proteasome produces fragments by canonical peptide-bond hydrolysis or by peptide splicing. Indeed, proteasome can break a protein and reshuffle its sequence by combining non-continuous fragments, thereby generating spliced peptides. By peptide splicing, proteasome significantly enlarges the antigenic landscape of a cell. We developed a novel method for spliced peptide identification and discovered its relevance in the antigen presentation.