Investigating signalling pathways regulating human trophectoderm development This project focuses on elucidating signalling pathways regulating trophectoderm development in the human embryo. My previous transcriptional studies of human blastocysts provide a resource for the identification of candidate pathways. We will use a combination of molecular tools and advanced imaging techniques to determine whether candidate pathways are active in the embryo. The functional role of candidate pathways will then be determined by blocking activity using precise molecular tools, and examining the effect on cell proliferation, gene expression and embryo development. As human embryos are a limited resource, we will take advantage of in vitro models to refine the molecular tools and techniques before applying them to embryos.