Light sheet microscopy illuminates a sample with a thin sheet of light from the side, while detection occurs perpendicular to the illumination plane. This setup excites only a single optical section at a time, reducing photobleaching and phototoxicity compared to conventional methods. It allows fast, high-contrast imaging of large specimens, making it particularly well suited for long-term imaging of developing organisms or large 3D samples. Light sheet imaging is available on out Leica Viventis DEEP dual view light sheet system.

 

Banner image: Organoid. Left: 40-h time-lapse of a human brain organoid.
Courtesy of Akanksha Jain, Treutlein Lab ETH-DBSSE Basel, CH.
Right: Z-stack image of a cleared 60-day-old human brain organoid.
Courtesy of Professor Barbara Treutlein, ETH, D-BSSE Basel, Switzerland.