This is a Fluxion/Cell Microsystems BioFlux 1000z microfluidic system combined with a Zeiss LSM900 confocal microscope with Airyscan 2 for studying microbial biofilm growth. The equipment can be run as an integrated system, which enables monitoring spatial/temporal biofilm growth and the effect of external factors (e.g. antimicrobials) on biofilm structures in real time. This equipment can also be used separately; for example, growing microfluidic biofilms off the microscope, and using the microscope to study other samples.
The BioFlux instrument allows for growth of microbial biofilms on a range of substrate materials and under precise environmental control (e.g. temperature, shear flow, gas, mixed/gradient media input). It uses a well plate format, ranging from 6-well, 24-well to 48-well, with integrated microfluidics, for the delivery of different initial volumes of inlet media, the use of single or dual inlet channels.
The Zeiss LSM900 is equipped with 4 lasers (405/488/561/639nm) and 4 objectives (10x/20x/40xW/63xO/100xO), 2x GaAsP confocal detectors, 1x transmitted light detector as well as an Airyscan 2 detector. It also has fluorescence for visualisation of DAPI, GFP, RFP or associated fluorophores.
Facility staff
Reader in Microbial Structural Biology
|
Equipment |
Rate (£/day) |
|||
|
UKRI |
Charity |
EC |
Industry |
|
|
BioFlux 1000z |
10.70 |
13.39 |
21.44 |
23.68 |
|
Zeiss LSM 900 |
14.51 |
17.21 |
52.13 |
57.34 |
|
Consumables |
Price (£/unit) |
|
BioFlux plates |
250.00 |
The BioFlux instrument allows for growth of microbial biofilms on a range of substrate materials and under precise environmental control (e.g. temperature, shear flow, gas, mixed/gradient media input). It uses a well plate format, ranging from 6-well, 24-well to 48-well, with integrated microfluidics, for the delivery of different initial volumes of inlet media, the use of single or dual inlet channels.
The Zeiss LSM900 is equipped with 4 lasers (405/488/561/639nm) and 4 objectives (10x/20x/40xW/63xO/100xO), 2x GaAsP confocal detectors, 1x transmitted light detector as well as an Airyscan 2 detector. It also has fluorescence for visualisation of DAPI, GFP, RFP or associated fluorophores.
Facility staff
Reader in Microbial Structural Biology
|
Equipment |
Rate (£/day) |
|||
|
UKRI |
Charity |
EC |
Industry |
|
|
BioFlux 1000z |
10.70 |
13.39 |
21.44 |
23.68 |
|
Zeiss LSM 900 |
14.51 |
17.21 |
52.13 |
57.34 |
|
Consumables |
Price (£/unit) |
|
BioFlux plates |
250.00 |
Partners
Biotechnology and Biological Sciences Research Council
Contact us
For more information regarding this piece of equipment please contact the Academic Lead, James Garnett, using the details below