Molecular Characterisation

Biacore Facility

The Biacore T100 instrument is a versatile biophysical tool for characterising molecular interactions, enabling real-time and label-free binding studies.

Access: Contact the facility lead.
Training: Training is provided on an individual basis.
Location: New Hunts House, Great Maze Pond, London, SE1 9RT | Guy’s Campus
Contact: Facility Lead: Professor Jim McDonnell

Centre for Biomolecular Spectroscopy

The Centre brings together advanced instrumentation in NMR and a number of other biophysical techniques to provide a set of facilities to promote the study of biological molecules, from proteins to metabolites, both in basic and medical science, and for projects that translate these advances into clinical understanding and benefit. It provides expertise and state-of-the-art biophysical facilities to King's research groups and external users.

Access: Contact Facility Lead
Training: : Training is available and assessed on an individual basis.
Location: New Hunts House, Great Maze Pond, London, SE1 9RT | Guy’s Campus
Contact: Facility Lead: James Jarvis

Chemistry Research Facility

The Chemistry Research Facility offers analytical services, including both Mass Spectrometry (MS) and Nuclear Magnetic Resonance (NMR). The instruments included in the service are a Q-TOF HRMS, GCMS and 400 MHz NMR spectrometer all managed by specialist instrument technicians. Applications for these instruments span from routine analysis, such as accurate mass measurements and structural characterisation, to more bespoke applications available on request. For more information, please reach out to discuss your research needs.

Access: Contact the facility manager for general enquiries.
Location: Britannia House, 7 Trinity Street, London SE1 1DB | Guy’s Campus
Contact: Facility Manager: Isabel Cruz-Gallardo | MS: Oscar Ayrton | NMR: Thomas Hicks

FACS Cell Sorter Facility - ARIAIIu

The FACS Facility offers a FACS with red, yellow/green, blue, violet and UV laser, capable to perform 2-way or 4-way cell sorting, and cell sorting into 6, 12, 24, 48 and 96 well plates. The FACS is operated by the facility manager.

Access: Contact the facility manager for enquiries and bookings.
Location: Rayne Institute, 123 Coldharbour Lane, London SE5 9NU | Denmark Hill Campus
Contact: Facility Manager: Tsz Kan Fung

FWB NMR Facility

The facility offers an open access 400 MHz spectrometer for small molecule NMR. After a safety induction, users receive an account to submit samples, which are run in automation.

Access: Users are given an account or use a group account to submit samples which are run in automation. Charges are either by the hour or a flat fee for annual access. Contact the facility manager to arrange access.
Training: The facility provides a safety induction and some tips on best practice but do not provide training in use or spectra interpretation.
Location: Franklin Wilkins Building, Stamford Street, London SE1 9NQ | Waterloo Campus
Contact: Facility Manager: James Mason

Isothermal Titration Calorimetry Facility

ITC is considered the gold standard for quantitative measurements of biomolecular interactions. A single ITC experiment allows the determination of binding affinities and stoichiometries, and the thermodynamic parameters (ΔH, ΔS) of an interaction. We offer access to three Malvern ITC instruments including the fully automated PEAQ-ITC.

Access: Contact the facility lead. 
Training: Training provided on an individual basis. 
Location: New Hunts House, Great Maze Pond, London, SE1 9RT | Guy’s Campus
Contact: Facility Lead: Professor Sasi Conte

Light Scattering Facility

The facility houses the latest version of Wyatt Technology's SEC-MALs (DAWN & OPTILAB, coupled with the Agilent's HPLC) and DynaPro Plate Reader instruments to study the molecular mass and size of biological molecules in solution phase.

Access: The facility is available to all King’s researchers. Contact the Centre for enquiries.
Training: Training is available upon request.
Location: Lab 3.3, New Hunt’s House, Great Maze Pond, London SE1 1UL | Guy’s Campus
Contact: Dr Tam Bui or Dr Julien Bergeron.

Mitochondrial Bioenergetics Users Facility

This facility enables users to undertake parallel analysis of the two major energy pathways in the cells mitochondrial respiration and glycolysis, in real time, quantifying oxidative phosphorylation, fatty acid oxidation, extracellular acidification, glycolysis, substrate utilisation evaluation and other key bioenergetic measurements in live cells. The instruments available are:

• Seahorse XFe96 Pro Extracellular Flux Analyzer.
• The Biotek Cytation 5, a cell imaging multimode reader, used for data normalisation. Further details of this instrument can be found on Agilent.

Visit Agilent for help with designing your experiments.

Access: Contact the Facility Manager to discuss access.
Training: Training is required for access to the facility and the equipment booking system. To book onto the next training sessions, email Aakruti Kaikini. Hands on training takes place over 2 days and is provided by an Agilent expert. This is followed by a data handling training session.
Location: Hodgkin Building, Great Maze Pond, London SE1 1UL | Guy’s Campus
Contact: Facility Manager: Dr Hannah Rosa

NMR Facility

The NMR Facility is a core research facility that provides researchers access to four state of the art, high-field spectrometers, supporting a range of experimental techniques dedicated largely to the study of biological systems, including investigating protein structure and dynamics, metabolomics and mixture analysis, small molecules, drug screening and biological solids.

Access: Contact the facility manager.
Training: Services are generally provided by facility staff but users can be trained to operate equipment independently.
Location: Hodgkin Building, Guy’s Campus, London, SE1 1UL
Contact: Facility Manager: James Jarvis

Optical Spectroscopy Facility

Chirascan and Chirascan Plus instruments are multimode spectrometers capable of measuring absorption, fluorescence and light scattering properties for ordinary, linearly and circularly polarised light for the same sample over the 170-1200 nm region. Applications include the assignment of absolute stereochemistry, the analysis of biopolymer conformation, quality control of biologicals, the study of molecular interactions and 1 ms stopped-flow kinetics.

Access: Contact the facility.
Training: Training is provided on an individual basis.
Location: New Hunts House, Great Maze Pond, London, SE1 9RT | Guy’s Campus
Contact: Facility Technician Tam Bui

Proteomics Facility

The Proteomics Facility is a core research facility that provides state of the art protein mass spectrometry support for researchers at King's and external institutions, offering a range of services from the simplest protein identification to very complex large scale biomarker discovery and verification studies in a wide range of biological matrices.

Access: Contact the Facility Manager for enquiries.
Training: All research work is carried out by experienced facility staff members. The facility hosts monthly informal drop-in clinics to discuss how proteomics can help researchers and potential projects.  
Location: The James Black Centre, 125 Coldharbour Lane London SE5 9NU | Denmark Hill Campus 
Contact: Facility Manager: Steven Lynham

X-ray Crystallography

X-ray crystallography analyses the x-ray diffraction patterns and quality of crystallisation to determine the atomic and molecular structure of protein in crystals.

Access: Contact the facility manager.
Training: Training is available and assessed on an individual basis.
Location: New Hunt’s House, Great Maze Pond, London, SE1 1UL | Guy’s Campus
Contact: Facility Manager: Risa Mori