The NMR Facility at King’s College London is a Core Facility providing researchers access to state-of-the-art high-field nuclear magnetic resonance (NMR) spectroscopy, together with expert scientific support. We work collaboratively with researchers at all stages of their projects, from experimental design and sample preparation through data acquisition, analysis and interpretation.
The facility supports a broad user base across the life and chemical sciences, enabling research in structural biology, metabolomics, chemical biology and small‑molecule characterisation. Our expertise covers the study of biomolecules including proteins, RNA and DNA, encompassing structure, dynamics and molecular interactions, as well as metabolic profiling of biofluids and tissues and the structural elucidation of small molecules and complex mixtures in both basic and translational research contexts.
The NMR Facility is part of the Centre for Biomolecular Spectroscopy (CBS), which integrates advanced NMR instrumentation with complementary biophysical techniques to promote multidisciplinary research on biological systems, from proteins and nucleic acids to metabolites. Through this integrated environment, users can access high‑quality infrastructure alongside specialist advice to maximise scientific impact.
Located in the Wolfson Wing of the Hodgkin Building on Guy’s Campus, the Facility is open to researchers from across King’s College London, as well as external academic collaborators and industry partners.
The NMR Facility provides advanced instrumentation and specialist expertise for the structural and dynamic analysis of biomolecules (including proteins, RNA and DNA), the study of metabolites and complex mixtures, and the characterisation of small molecules.
Structural Biology
NMR based investigations of biomolecular structure, dynamics and interactions, including proteins, RNA, DNA and protein–nucleic acid complexes.
Metabolomics
The Facility supports NMR metabolomics studies across a wide range of biological samples, including biofluids, aqueous and lipid extracts, and intact tissues.
Small Molecules & Chemical Biology
The NMR Facility supports a wide range of small molecule and chemical biology applications, providing access to high quality solution state NMR spectroscopy and expert guidance.
Facility staff
NMR Facility Manager
NMR Facility Scientific Officer
Affiliated academics
Professor of Structural Biology
Lecturer
The NMR Facility houses a comprehensive suite of high‑field NMR spectrometers operating at 400 MHz, 600 MHz, 700 MHz and 800 MHz. These instruments support a wide range of solution‑state NMR applications across structural biology, metabolomics and small‑molecule research. The laboratory is complemented by automated sample handling and preparation systems, enabling reproducible and efficient workflows from small‑scale studies to medium‑throughput analyses.
Related equipment
400 MHz NMR Spectrometer
A versatile instrument for solution state NMR applications
600 MHz NMR Spectrometer
A high field instrument optimised for NMR metabolomics
700 MHz NMR Spectrometer
A flexible high field system for structural biology, small molecule research and automated workflows
800 MHz NMR Spectrometer
A state-of-the-art instrument for protein structural biology
Bruker SamplePro Tube
Automated liquid handling for NMR sample preparation
2026
Capraro, F. et al. (2026) ‘An intrinsically disordered region mediates RNA‑binding selectivity and cellular activities of LARP6’, Nature Communications, 17, Article 2939. https://doi.org/10.1038/s41467-026-69789-z
Pellon, A. et al. (2026) ‘Fungal infection drives metabolic reprogramming in epithelial cells via aerobic glycolysis and an alternative TCA cycle shunt’, Science Advances, 12(6), eaea0405. https://doi.org/10.1126/sciadv.aea0405.
Squire, I. et al. (2026) ‘A neutral cyclic aluminium (I) trimer’, Nature Communications, 17, Article 1732. https://doi.org/10.1038/s41467-026-68432-1.
2025
Adams, C.O. et al. (2025) ‘Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin‑ and serum‑mediated killing’, mBio, 16, e01393‑25. https://doi.org/10.1128/mbio.01393-25.
Brian, J.I. et al. (2025) ‘Neighbours, not consumers, drive local intraspecific phytochemical changes of two grassland species’, AoB PLANTS, 17, plaf071. https://doi.org/10.1093/aobpla/plaf071.
Costanzo, H. et al. (2025) ‘In silico and in vitro characterisation and affinity maturation of human red blood cell binding aptamers’, RSC Advances, 15, pp. 22505–22523. https://doi.org/10.1039/D5RA00645G.
Everett, J.R. et al. (2025) ‘Clinical and metabolic phenotypes of Oxford Biobank subjects with variations in human flavin‑containing monooxygenase 5 (FMO5)’, Metabolomics, 21, 135. https://doi.org/10.1007/s11306-025-02308-1.
Zhang, Y. et al. (2025) ‘Successful endodontic treatment improves glucose and lipid metabolism: a longitudinal metabolomic study’, Journal of Translational Medicine, 23, Article 1195. https://doi.org/10.1186/s12967-025-07110-0.
2024
Chen, S.W. et al. (2024) ‘Structure–Toxicity Relationship in Intermediate Fibrils from α-Synuclein Condensates’, Journal of the American Chemical Society, 146(15), pp. 10537–10549. https://doi.org/10.1021/jacs.3c14703.
Diez‑Guardia, V. et al. (2024) ‘Controlled release of human dental pulp stem cell‑derived exosomes from hydrogels attenuates temporomandibular joint osteoarthritis’, Advanced Healthcare Materials, 14(31), 2402923. https://doi.org/10.1002/adhm.202402923.
Ding, Y. et al. (2024) ‘Rapid Peptide Cyclization Inspired by the Modular Logic of Nonribosomal Peptide Synthetases’, Journal of the American Chemical Society, 146(24), pp. 16787–16801. https://doi.org/10.1021/jacs.4c04711
Fernandes, W.M. et al. (2024) ‘High‑resolution magic angle spinning nuclear magnetic resonance spectroscopy of paired clinical liver tissue samples from hepatocellular cancer and surrounding region’, International Journal of Molecular Sciences, 25(16), 8924. https://doi.org/10.3390/ijms25168924.
Michaels, A.M. et al. (2024) ‘Disrupting Na+ ion homeostasis and Na+/K+ ATPase activity in breast cancer cells directly modulates glycolysis in vitro and in vivo’, Cancer & Metabolism, 12(1), p. 15. https://doi.org/10.1186/s40170-024-00343-5.
Rehman, S. et al. (2024) ‘The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans’, Nature Communications, 15(1), p. 4912. https://doi.org/10.1038/s41467-024-49255-4.
Yu, H. et al. (2024) ‘Injectable PEG Hydrogels with Tissue-Like Viscoelasticity Formed through Reversible Alendronate–Calcium Phosphate Crosslinking for Cell–Material Interactions’, Advanced Healthcare Materials, 13(22), p. 2400472. https://doi.org/10.1002/adhm.202400472.
2023
Alamri, M.M. et al. (2023) ‘Metabolomics analysis in saliva from periodontally healthy, gingivitis and periodontitis patients’, Journal of Periodontal Research, 58(6), pp. 1272–1280. https://doi.org/10.1111/jre.13183.
Augustin, A. et al. (2023) ‘Faecal metabolite deficit, gut inflammation and diet in Parkinson’s disease: Integrative analysis indicates inflammatory response syndrome’, Clinical and Translational Medicine, 13(1), p. e1152. https://doi.org/10.1002/ctm2.1152.
Clarke, M. et al. (2023) ‘Synergy between winter flounder antimicrobial peptides’, npj Antimicrobials and Resistance, 1, Article 8. https://doi.org/10.1038/s44259-023-00008-4.
Cleaver, L.M. et al. (2023) ‘Novel bacterial proteolytic and metabolic activity associated with dental erosion-induced oral dysbiosis’, Microbiome, 11(1), p. 69. https://doi.org/10.1186/s40168-023-01514-0.
Graziani, V. et al. (2023) ‘Metabolic rewiring in MYC-driven medulloblastoma by BET-bromodomain inhibition’, Scientific Reports, 13(1), p. 1273. https://doi.org/10.1038/s41598-023-27375-z.
Hadjihambi, A. et al. (2023) ‘Partial MCT1 invalidation protects against diet-induced non-alcoholic fatty liver disease and the associated brain dysfunction’, Journal of Hepatology, 78(1), pp. 180–190. https://doi.org/10.1016/j.jhep.2022.08.008.
Hungnes, I.N. et al. (2023) ‘Versatile Diphosphine Chelators for Radiolabeling Peptides with 99mTc and 64Cu’, Inorganic Chemistry, 62(50), pp. 20608–20620. https://doi.org/10.1021/acs.inorgchem.3c00426.
Parijat, P. et al. (2023) ‘Discovery of novel cardiac troponin activators using fluorescence polarization-based high throughput screening assays’, Scientific Reports, 13(1), p. 5216. https://doi.org/10.1038/s41598-023-32476-w.
Rivas, C. et al. (2023) ‘Probing Unexpected Reactivity in Radiometal Chemistry: Indium-111-Mediated Hydrolysis of Hybrid Cyclen-Hydroxypyridinone Ligands’, Inorganic Chemistry, 62(13), pp. 5270–5281. https://doi.org/10.1021/acs.inorgchem.3c00353.
Zhang, W. et al. (2023) ‘Characterising the chemical and physical properties of phase-change nanodroplets’, Ultrasonics Sonochemistry, 97, p. 106445. https://doi.org/10.1016/j.ultsonch.2023.106445.
2022
Foratori‑Junior, G.A. et al. (2022) ‘Metabolomic profiles associated with obesity and periodontitis during pregnancy: a cross‑sectional 1H‑NMR‑based study’, Metabolites, 12(11). https://doi.org/10.3390/metabo12111029
Assante, G. et al. (2022) ‘Reduced circulating FABP2 in patients with moderate to severe COVID‑19 may indicate enterocyte functional change rather than cell death’, Scientific Reports, 12, 18792. doi: 10.1038/s41598-022-23282-x
Cox, I.J. et al. (2022) ‘Stool microbiota show greater linkages with plasma metabolites compared to salivary microbiota in a multinational cirrhosis cohort’, Liver International, 42, pp. 2274–2282. https://doi.org/10.1111/liv.15329
Madeira, P.C. et al. (2022) ‘Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation’, npj Biofilms and Microbiomes, 8(1). https://doi.org/10.1038/s41522-022-00272-5
Horrocks, V. et al. (2022) ‘Nuclear magnetic resonance metabolomics of symbioses between bacterial vaginosis‑associated bacteria’, mSphere, 7(3). DOI: 10.1128/msphere.00166-22
Switzer, C.H., Cho, H., Eykyn, T.R., Lavender, P. and Eaton, P. (2022) ‘NOS2 and S‑nitrosothiol signalling induces DNA hypomethylation and LINE‑1 retrotransposon expression’, Proceedings of the National Academy of Sciences, 119(21). DOI: 10.1073/pnas.2200022119
The NMR Facility supports collaborative research by providing access to advanced NMR instrumentation and specialist expertise. The Facility is available to researchers across King’s College London, with access for external academic and industry users considered subject to capacity and project suitability.
Researchers interested in using the NMR Facility are encouraged to contact the team at nmr@kcl.ac.uk to discuss their proposed project. Facility staff will advise on experimental feasibility, appropriate NMR methodologies, and any training requirements prior to access.
Booking equipment
All instrument bookings must be made through the Stratacore booking system. Trained users may request out‑of‑hours access (Monday–Friday, 09:00–17:00) subject to staff approval and demonstrated ability to operate independently.
Users are responsible for backing up their own data and for ensuring that all samples brought into the Facility are appropriately risk‑assessed, compatible with NMR analysis and clearly labelled. All waste must be removed by users after each session.
Publications and acknowledgement
Research outputs that include data generated using the NMR Facility must acknowledge the Facility and its funders. Where NMR Facility staff contribute substantially to experimental design, data acquisition or interpretation, appropriate recognition through authorship is expected in line with King’s Core Facilities publication guidelines.
The NMR Facility provides advanced instrumentation and specialist expertise for the structural and dynamic analysis of biomolecules (including proteins, RNA and DNA), the study of metabolites and complex mixtures, and the characterisation of small molecules.
Structural Biology
NMR based investigations of biomolecular structure, dynamics and interactions, including proteins, RNA, DNA and protein–nucleic acid complexes.
Metabolomics
The Facility supports NMR metabolomics studies across a wide range of biological samples, including biofluids, aqueous and lipid extracts, and intact tissues.
Small Molecules & Chemical Biology
The NMR Facility supports a wide range of small molecule and chemical biology applications, providing access to high quality solution state NMR spectroscopy and expert guidance.
Facility staff
NMR Facility Manager
NMR Facility Scientific Officer
Affiliated academics
Professor of Structural Biology
Lecturer
The NMR Facility houses a comprehensive suite of high‑field NMR spectrometers operating at 400 MHz, 600 MHz, 700 MHz and 800 MHz. These instruments support a wide range of solution‑state NMR applications across structural biology, metabolomics and small‑molecule research. The laboratory is complemented by automated sample handling and preparation systems, enabling reproducible and efficient workflows from small‑scale studies to medium‑throughput analyses.
Related equipment
400 MHz NMR Spectrometer
A versatile instrument for solution state NMR applications
600 MHz NMR Spectrometer
A high field instrument optimised for NMR metabolomics
700 MHz NMR Spectrometer
A flexible high field system for structural biology, small molecule research and automated workflows
800 MHz NMR Spectrometer
A state-of-the-art instrument for protein structural biology
Bruker SamplePro Tube
Automated liquid handling for NMR sample preparation
2026
Capraro, F. et al. (2026) ‘An intrinsically disordered region mediates RNA‑binding selectivity and cellular activities of LARP6’, Nature Communications, 17, Article 2939. https://doi.org/10.1038/s41467-026-69789-z
Pellon, A. et al. (2026) ‘Fungal infection drives metabolic reprogramming in epithelial cells via aerobic glycolysis and an alternative TCA cycle shunt’, Science Advances, 12(6), eaea0405. https://doi.org/10.1126/sciadv.aea0405.
Squire, I. et al. (2026) ‘A neutral cyclic aluminium (I) trimer’, Nature Communications, 17, Article 1732. https://doi.org/10.1038/s41467-026-68432-1.
2025
Adams, C.O. et al. (2025) ‘Legionella pneumophila type II secretome reveals a polysaccharide deacetylase that impacts intracellular infection, biofilm formation, and resistance to polymyxin‑ and serum‑mediated killing’, mBio, 16, e01393‑25. https://doi.org/10.1128/mbio.01393-25.
Brian, J.I. et al. (2025) ‘Neighbours, not consumers, drive local intraspecific phytochemical changes of two grassland species’, AoB PLANTS, 17, plaf071. https://doi.org/10.1093/aobpla/plaf071.
Costanzo, H. et al. (2025) ‘In silico and in vitro characterisation and affinity maturation of human red blood cell binding aptamers’, RSC Advances, 15, pp. 22505–22523. https://doi.org/10.1039/D5RA00645G.
Everett, J.R. et al. (2025) ‘Clinical and metabolic phenotypes of Oxford Biobank subjects with variations in human flavin‑containing monooxygenase 5 (FMO5)’, Metabolomics, 21, 135. https://doi.org/10.1007/s11306-025-02308-1.
Zhang, Y. et al. (2025) ‘Successful endodontic treatment improves glucose and lipid metabolism: a longitudinal metabolomic study’, Journal of Translational Medicine, 23, Article 1195. https://doi.org/10.1186/s12967-025-07110-0.
2024
Chen, S.W. et al. (2024) ‘Structure–Toxicity Relationship in Intermediate Fibrils from α-Synuclein Condensates’, Journal of the American Chemical Society, 146(15), pp. 10537–10549. https://doi.org/10.1021/jacs.3c14703.
Diez‑Guardia, V. et al. (2024) ‘Controlled release of human dental pulp stem cell‑derived exosomes from hydrogels attenuates temporomandibular joint osteoarthritis’, Advanced Healthcare Materials, 14(31), 2402923. https://doi.org/10.1002/adhm.202402923.
Ding, Y. et al. (2024) ‘Rapid Peptide Cyclization Inspired by the Modular Logic of Nonribosomal Peptide Synthetases’, Journal of the American Chemical Society, 146(24), pp. 16787–16801. https://doi.org/10.1021/jacs.4c04711
Fernandes, W.M. et al. (2024) ‘High‑resolution magic angle spinning nuclear magnetic resonance spectroscopy of paired clinical liver tissue samples from hepatocellular cancer and surrounding region’, International Journal of Molecular Sciences, 25(16), 8924. https://doi.org/10.3390/ijms25168924.
Michaels, A.M. et al. (2024) ‘Disrupting Na+ ion homeostasis and Na+/K+ ATPase activity in breast cancer cells directly modulates glycolysis in vitro and in vivo’, Cancer & Metabolism, 12(1), p. 15. https://doi.org/10.1186/s40170-024-00343-5.
Rehman, S. et al. (2024) ‘The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans’, Nature Communications, 15(1), p. 4912. https://doi.org/10.1038/s41467-024-49255-4.
Yu, H. et al. (2024) ‘Injectable PEG Hydrogels with Tissue-Like Viscoelasticity Formed through Reversible Alendronate–Calcium Phosphate Crosslinking for Cell–Material Interactions’, Advanced Healthcare Materials, 13(22), p. 2400472. https://doi.org/10.1002/adhm.202400472.
2023
Alamri, M.M. et al. (2023) ‘Metabolomics analysis in saliva from periodontally healthy, gingivitis and periodontitis patients’, Journal of Periodontal Research, 58(6), pp. 1272–1280. https://doi.org/10.1111/jre.13183.
Augustin, A. et al. (2023) ‘Faecal metabolite deficit, gut inflammation and diet in Parkinson’s disease: Integrative analysis indicates inflammatory response syndrome’, Clinical and Translational Medicine, 13(1), p. e1152. https://doi.org/10.1002/ctm2.1152.
Clarke, M. et al. (2023) ‘Synergy between winter flounder antimicrobial peptides’, npj Antimicrobials and Resistance, 1, Article 8. https://doi.org/10.1038/s44259-023-00008-4.
Cleaver, L.M. et al. (2023) ‘Novel bacterial proteolytic and metabolic activity associated with dental erosion-induced oral dysbiosis’, Microbiome, 11(1), p. 69. https://doi.org/10.1186/s40168-023-01514-0.
Graziani, V. et al. (2023) ‘Metabolic rewiring in MYC-driven medulloblastoma by BET-bromodomain inhibition’, Scientific Reports, 13(1), p. 1273. https://doi.org/10.1038/s41598-023-27375-z.
Hadjihambi, A. et al. (2023) ‘Partial MCT1 invalidation protects against diet-induced non-alcoholic fatty liver disease and the associated brain dysfunction’, Journal of Hepatology, 78(1), pp. 180–190. https://doi.org/10.1016/j.jhep.2022.08.008.
Hungnes, I.N. et al. (2023) ‘Versatile Diphosphine Chelators for Radiolabeling Peptides with 99mTc and 64Cu’, Inorganic Chemistry, 62(50), pp. 20608–20620. https://doi.org/10.1021/acs.inorgchem.3c00426.
Parijat, P. et al. (2023) ‘Discovery of novel cardiac troponin activators using fluorescence polarization-based high throughput screening assays’, Scientific Reports, 13(1), p. 5216. https://doi.org/10.1038/s41598-023-32476-w.
Rivas, C. et al. (2023) ‘Probing Unexpected Reactivity in Radiometal Chemistry: Indium-111-Mediated Hydrolysis of Hybrid Cyclen-Hydroxypyridinone Ligands’, Inorganic Chemistry, 62(13), pp. 5270–5281. https://doi.org/10.1021/acs.inorgchem.3c00353.
Zhang, W. et al. (2023) ‘Characterising the chemical and physical properties of phase-change nanodroplets’, Ultrasonics Sonochemistry, 97, p. 106445. https://doi.org/10.1016/j.ultsonch.2023.106445.
2022
Foratori‑Junior, G.A. et al. (2022) ‘Metabolomic profiles associated with obesity and periodontitis during pregnancy: a cross‑sectional 1H‑NMR‑based study’, Metabolites, 12(11). https://doi.org/10.3390/metabo12111029
Assante, G. et al. (2022) ‘Reduced circulating FABP2 in patients with moderate to severe COVID‑19 may indicate enterocyte functional change rather than cell death’, Scientific Reports, 12, 18792. doi: 10.1038/s41598-022-23282-x
Cox, I.J. et al. (2022) ‘Stool microbiota show greater linkages with plasma metabolites compared to salivary microbiota in a multinational cirrhosis cohort’, Liver International, 42, pp. 2274–2282. https://doi.org/10.1111/liv.15329
Madeira, P.C. et al. (2022) ‘Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation’, npj Biofilms and Microbiomes, 8(1). https://doi.org/10.1038/s41522-022-00272-5
Horrocks, V. et al. (2022) ‘Nuclear magnetic resonance metabolomics of symbioses between bacterial vaginosis‑associated bacteria’, mSphere, 7(3). DOI: 10.1128/msphere.00166-22
Switzer, C.H., Cho, H., Eykyn, T.R., Lavender, P. and Eaton, P. (2022) ‘NOS2 and S‑nitrosothiol signalling induces DNA hypomethylation and LINE‑1 retrotransposon expression’, Proceedings of the National Academy of Sciences, 119(21). DOI: 10.1073/pnas.2200022119
The NMR Facility supports collaborative research by providing access to advanced NMR instrumentation and specialist expertise. The Facility is available to researchers across King’s College London, with access for external academic and industry users considered subject to capacity and project suitability.
Researchers interested in using the NMR Facility are encouraged to contact the team at nmr@kcl.ac.uk to discuss their proposed project. Facility staff will advise on experimental feasibility, appropriate NMR methodologies, and any training requirements prior to access.
Booking equipment
All instrument bookings must be made through the Stratacore booking system. Trained users may request out‑of‑hours access (Monday–Friday, 09:00–17:00) subject to staff approval and demonstrated ability to operate independently.
Users are responsible for backing up their own data and for ensuring that all samples brought into the Facility are appropriately risk‑assessed, compatible with NMR analysis and clearly labelled. All waste must be removed by users after each session.
Publications and acknowledgement
Research outputs that include data generated using the NMR Facility must acknowledge the Facility and its funders. Where NMR Facility staff contribute substantially to experimental design, data acquisition or interpretation, appropriate recognition through authorship is expected in line with King’s Core Facilities publication guidelines.
Contact us
Wolfson SPaRC (WWB.16), Hodgkin Building Guy's Campus King’s College London London, SE1 1UL