The NMR Facility provides King’s researchers with access to state-of-the-art high-field nuclear magnetic resonance spectrometers and provides expert support and expertise to users conducting a wide variety of biological and chemical research.
The Facility is part of the Centre for Biomolecular Spectroscopy (CBS), which brings together advanced instrumentation in NMR and other biophysical techniques to provide a set of facilities to promote the study of biological molecules, from proteins to metabolites, both in basic and medical science.
These facilities offer researchers a pool of expertise in biomolecular NMR spectroscopy and provide technical training and analytical support. Other facilities connected to the Centre for Biomolecular Spectroscopy are listed in the links sections.
The NMR Facility is located in the basement of the Wolfson Wing of the Hodgkin Building on Guy’s Campus.
The NMR Facility provides advanced instrumentation for the investigation of protein structure, dynamics and interactions, as well as analysis of metabolites and mixtures, and structural elucidation of small molecules.
Structural Biology
The CSB NMR Facility is home to state-of-the-art equipment for investigating the structure and dynamics of proteins. We offer users two high field instruments at 700 and 800MHz dedicated and optimised for structural biology applications. These are equipped with helium cooled cyroprobes for optimal sensitivity. We offer users a full suite of modern experiments for protein assignment, structure determination, and relaxation and dynamics studies. Our 700MHz instrument has a 4-channel QCI-P cryoprobe with a 31P channel that allows users to extend the scope of their structural biology projects to include nucleic acids.
Metabolomics
The CBS NMR Facility provides access to advanced equipment for NMR metabolomics studies. Our 600MHz and 700MHz NMR instruments are equipped with cryogenically cooled probes, offering excellent sensitivity for studies of various biofluids, and aqueous and lipid cell extracts. Both also have SampleJet automated sample changers that accept up to 5 racks of 96 3mm or 5mm NMR tubes for automated medium-throughput analysis of large numbers of samples. We also offer users access to our SamplePro liquid handling robot, allowing for the efficient and automated preparation of large numbers of NMR samples for analysis. The 600MHz NMR also has a HR-MAS probe available for metabolic analysis of intact tissues.
Facility staff
James Jarvis
NMR Facility Manager
Adrien Le Guennec
NMR Facility Scientific Officer
Sasi Conte
Professor of Structural Biology
Affiliated academics
Tam Bui
Research Associate
Mark Pfuhl
Reader
James Garnett
Senior Lecturer in Structural Bacteriology
Rivka Isaacson
Associate Dean for Doctoral Studies and Reader in Chemical Biology
James Mason
Professor of Membrane Biochemistry
Thomas Eykyn
Lecturer
Po-Wah So
Senior Lecturer
Guy Carpenter
Professor of Oral Biology
James McDonnell
Professor of Molecular Immunology
The NMR laboratory houses 400 MHz, 600 MHz, 700 MHz and 800 MHz NMR spectrometers, equipped for a range of applications.
The laboratory was refurbished in 2018, allowing for the purchase of the 600 MHz and 800 MHz NMR spectrometers. These state-of-the-art instruments have increased our capabilities, expanding both the range and volume of work that can be undertaken by King’s researchers in the NMR Facility.
Related equipment
400 MHz NMR Spectrometer
Equipped with Avance NEO console
600 MHz NMR Spectrometer
Equipped with Avance NEO console 1H,13C,15N TCI prodigy nitrogen-cooled cryoprobe 1H, 13C HR-MAS probe SampleJet sample changer (5x96 tubes)
700 MHz NMR Spectrometer
Equipped with Avance III console 1H,13C,15N, 31P QCI helium-cooled cryoprobe SampleJet sample changer (5x96 tubes)
800 MHz NMR Spectrometer
Equipped with Avance NEO console 1H,13C,15N TCI helium-cooled cryoprobe
Bruker SamplePro Tube
The SamplePro Tube is a XYZ robotic system for general liquid handling tasks to support sample preparation.
2022 Publications
Christopher H. Switzer, Hyun-Ju Cho, Thomas R. Eykyn, Paul Lavender, Philip Eaton. (2022) NOS2 and S-nitrosothiol signaling induces DNA hypomethylation and LINE-1 retrotransposon expression. PNAS 119(21).
Victoria Horrocks, Charlotte K. Hind, Matthew E. Wand, Paul-Enguerrand Fady, Joel Chan, Jade Hopkins, Georgina Houston, Rachel Tribe, John Sutton, James Mason. (2022) Nuclear magnetic resonance metabolomics of symbioses between bacterial vaginosis associated bacteria. mSphere 7(3).
Paula Corsini Madeira, Sunjun Wang, Saima Rehman, Katherine Fenn, Amin Sagar, Slobadan Sirovica, Leanne Cleaver, Charlotte Edwards-Gayle, Giulia Mastroianni, Benjamin James Dorgan, Lee Sewell, Steven Lynham, Dinu Iuga, W. Trents Franks, James Jarvis, Guy Carpenter, Michael Curtis, Pau Bernado, Vidya Darbari, James Garnett. (2022) Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation. npj Biofilms and Microbiomes 8(1).
2021 Publications
Ingebjorg Hungnes, Fahad Al Salemee, Peter Gawne, Thomas R Eykyn, Andrew Atkinson, Samantha Terry, Fiona Clarke, Philip J Blower, Paul G. Pringle, Michelle Ma. (2021) One-step, kit-based radiopharmaceuticals for molecular SPECT imaging: a versatile diphosphine chelator for 99mTc radiolabelling of peptides. Dalton Transactions 50(44).
Yu Jin Chung, Kyung Chan Park, Sergiy Tokar, Thomas R Eykyn, William Fuller, Davor Pavlovic, Pawel Swietach, Michael J Shattock. (2021) Off-target effects of sodium-glucose co-transporter 2 blockers: empagliflozin does not inhibit Na+/H+ exchanger-1 or lower [Na+]i in the heart. Cardiovasc Res 117(14) pp2794-2806.
Lucy J. Bock, Philip Ferguson, Maria Clarke, Vichayanee Pumpitakkul, Matthew E. Wand, Paul-Enguerrand Fady, Leanne Allison, Roland Fleck, Matthew Shepherd, James Mason, J. Mark Sutton. (2021) Pseudomonas aeruginosa adapts to octenidine via a combination of efflux and membrane remodelling. Communications Biology 4(1).
Selley, L., Lammers, A., Le Guennec, A., Pirhadi, M., Sioutas, C., Janssen, N., Maitland-van der Zee, A. H., Mudway, I., & Cassee, F. (2021) Alterations to the urinary metabolome following semi-controlled short exposures to ultrafine particles at a major airport. Int. J. Hyg. Environ. Health, 237, 113803.
Cleaver, L. M., Moazzez, R. V., & Carpenter, G. H. (2021) Evidence for Proline Utilization by Oral Bacterial Biofilms Grown in Saliva. Frontiers in microbiology, 11, 619968.
Flavia Flaviani, Natasha L. Hezelgrave, Tokuwa Kanno, Erica M. Prosdocimi, Evonne Chin-Smith, Alexandra E. Ridout, Djuna K. von Maydell, Vikash Mistry, William G. Wade, Andrew H. Shennan, Konstantina Dimitrakopoulou, Paul T. Seed, A. James Mason, Rachel M. Tribe (2021) Cervicovaginal microbiota and metabolome predict preterm birth risk in an ethnically diverse cohort. JCI Insight. 2021;6(16):e149257
Giorgia Manzo, Federico Gianfanti, Charlotte K. Hind, Leanne Allison, Maria Clarke, Julia Hohenbichler, Ilene Limantoro, Bethany Martin, Phoebe Do Carmo Silva, Philip M. Ferguson, Alice C. Hodgson-Casson, Roland A. Fleck, J. Mark Sutton, David A. Phoenix, and A. James Mason (2021) Impacts of Metabolism and Organic Acids on Cell Wall Composition and Pseudomonas aeruginosa Susceptibility to Membrane Active Antimicrobials. ACS Infectious Diseases 7(8), 2310-2323
Young, J. D., Ma, M. T., Eykyn, T. R., Atkinson, R. A., Abbate, V., Cilibrizzi, A., Hider, R. C., & Blower, P. J. (2021) Dipeptide inhibitors of the prostate specific membrane antigen (PSMA): A comparison of urea and thiourea derivatives. Bioorganic & medicinal chemistry letters, 42, 128044.
Matthew Farleigh, Truc Thuy Pham, Zilin Yu, Jana Kim, Kavitha Sunassee, George Firth, Nafsika Forte, Vijay Chudasama, James R. Baker, Nicholas J. Long, Charlotte Rivas, and Michelle T. Ma (2021) New Bifunctional Chelators Incorporating Dibromomaleimide Groups for Radiolabeling of Antibodies with Positron Emission Tomography Imaging Radioisotopes. Bioconjugate Chemistry 32 (7), 1214-1222
F Dudás, E., Puglisi, R., Korn, S. M., Alfano, C., Bellone, M. L., Piaz, F. D., Kelly, G., Monaca, E., Schlundt, A., Schwalbe, H., & Pastore, A. (2021) Backbone chemical shift spectral assignments of SARS coronavirus-2 non-structural protein nsp9. Journal of Biomolecular NMR assignments, 15(2), 235–241.
Ilkow, V. F., Davies, A. M., Dhaliwal, B., Beavil, A. J., Sutton, B. J., & McDonnell, J. M. (2021) Reviving lost binding sites: Exploring calcium-binding site transitions between human and murine CD23. FEBS open bio, 11(7), 1827–1840.
Colleen Kelly, Nicola Pace, Matthew Gage, Mark Pfuhl (2021) Solution NMR Structure of Titin N2A Region Ig Domain I83 and Its Interaction with Metal Ions. Journal of Molecular Biology 433(13)
Rees, M., Nikoopour, R., Fukuzawa, A. et al. (2021) Making sense of missense variants in TTN-related congenital myopathies. Acta Neuropathol 141, 431–453.
The NMR Facility provides advanced instrumentation for the investigation of protein structure, dynamics and interactions, as well as analysis of metabolites and mixtures, and structural elucidation of small molecules.
Structural Biology
The CSB NMR Facility is home to state-of-the-art equipment for investigating the structure and dynamics of proteins. We offer users two high field instruments at 700 and 800MHz dedicated and optimised for structural biology applications. These are equipped with helium cooled cyroprobes for optimal sensitivity. We offer users a full suite of modern experiments for protein assignment, structure determination, and relaxation and dynamics studies. Our 700MHz instrument has a 4-channel QCI-P cryoprobe with a 31P channel that allows users to extend the scope of their structural biology projects to include nucleic acids.
Metabolomics
The CBS NMR Facility provides access to advanced equipment for NMR metabolomics studies. Our 600MHz and 700MHz NMR instruments are equipped with cryogenically cooled probes, offering excellent sensitivity for studies of various biofluids, and aqueous and lipid cell extracts. Both also have SampleJet automated sample changers that accept up to 5 racks of 96 3mm or 5mm NMR tubes for automated medium-throughput analysis of large numbers of samples. We also offer users access to our SamplePro liquid handling robot, allowing for the efficient and automated preparation of large numbers of NMR samples for analysis. The 600MHz NMR also has a HR-MAS probe available for metabolic analysis of intact tissues.
Facility staff
James Jarvis
NMR Facility Manager
Adrien Le Guennec
NMR Facility Scientific Officer
Sasi Conte
Professor of Structural Biology
Affiliated academics
Tam Bui
Research Associate
Mark Pfuhl
Reader
James Garnett
Senior Lecturer in Structural Bacteriology
Rivka Isaacson
Associate Dean for Doctoral Studies and Reader in Chemical Biology
James Mason
Professor of Membrane Biochemistry
Thomas Eykyn
Lecturer
Po-Wah So
Senior Lecturer
Guy Carpenter
Professor of Oral Biology
James McDonnell
Professor of Molecular Immunology
The NMR laboratory houses 400 MHz, 600 MHz, 700 MHz and 800 MHz NMR spectrometers, equipped for a range of applications.
The laboratory was refurbished in 2018, allowing for the purchase of the 600 MHz and 800 MHz NMR spectrometers. These state-of-the-art instruments have increased our capabilities, expanding both the range and volume of work that can be undertaken by King’s researchers in the NMR Facility.
Related equipment
400 MHz NMR Spectrometer
Equipped with Avance NEO console
600 MHz NMR Spectrometer
Equipped with Avance NEO console 1H,13C,15N TCI prodigy nitrogen-cooled cryoprobe 1H, 13C HR-MAS probe SampleJet sample changer (5x96 tubes)
700 MHz NMR Spectrometer
Equipped with Avance III console 1H,13C,15N, 31P QCI helium-cooled cryoprobe SampleJet sample changer (5x96 tubes)
800 MHz NMR Spectrometer
Equipped with Avance NEO console 1H,13C,15N TCI helium-cooled cryoprobe
Bruker SamplePro Tube
The SamplePro Tube is a XYZ robotic system for general liquid handling tasks to support sample preparation.
2022 Publications
Christopher H. Switzer, Hyun-Ju Cho, Thomas R. Eykyn, Paul Lavender, Philip Eaton. (2022) NOS2 and S-nitrosothiol signaling induces DNA hypomethylation and LINE-1 retrotransposon expression. PNAS 119(21).
Victoria Horrocks, Charlotte K. Hind, Matthew E. Wand, Paul-Enguerrand Fady, Joel Chan, Jade Hopkins, Georgina Houston, Rachel Tribe, John Sutton, James Mason. (2022) Nuclear magnetic resonance metabolomics of symbioses between bacterial vaginosis associated bacteria. mSphere 7(3).
Paula Corsini Madeira, Sunjun Wang, Saima Rehman, Katherine Fenn, Amin Sagar, Slobadan Sirovica, Leanne Cleaver, Charlotte Edwards-Gayle, Giulia Mastroianni, Benjamin James Dorgan, Lee Sewell, Steven Lynham, Dinu Iuga, W. Trents Franks, James Jarvis, Guy Carpenter, Michael Curtis, Pau Bernado, Vidya Darbari, James Garnett. (2022) Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation. npj Biofilms and Microbiomes 8(1).
2021 Publications
Ingebjorg Hungnes, Fahad Al Salemee, Peter Gawne, Thomas R Eykyn, Andrew Atkinson, Samantha Terry, Fiona Clarke, Philip J Blower, Paul G. Pringle, Michelle Ma. (2021) One-step, kit-based radiopharmaceuticals for molecular SPECT imaging: a versatile diphosphine chelator for 99mTc radiolabelling of peptides. Dalton Transactions 50(44).
Yu Jin Chung, Kyung Chan Park, Sergiy Tokar, Thomas R Eykyn, William Fuller, Davor Pavlovic, Pawel Swietach, Michael J Shattock. (2021) Off-target effects of sodium-glucose co-transporter 2 blockers: empagliflozin does not inhibit Na+/H+ exchanger-1 or lower [Na+]i in the heart. Cardiovasc Res 117(14) pp2794-2806.
Lucy J. Bock, Philip Ferguson, Maria Clarke, Vichayanee Pumpitakkul, Matthew E. Wand, Paul-Enguerrand Fady, Leanne Allison, Roland Fleck, Matthew Shepherd, James Mason, J. Mark Sutton. (2021) Pseudomonas aeruginosa adapts to octenidine via a combination of efflux and membrane remodelling. Communications Biology 4(1).
Selley, L., Lammers, A., Le Guennec, A., Pirhadi, M., Sioutas, C., Janssen, N., Maitland-van der Zee, A. H., Mudway, I., & Cassee, F. (2021) Alterations to the urinary metabolome following semi-controlled short exposures to ultrafine particles at a major airport. Int. J. Hyg. Environ. Health, 237, 113803.
Cleaver, L. M., Moazzez, R. V., & Carpenter, G. H. (2021) Evidence for Proline Utilization by Oral Bacterial Biofilms Grown in Saliva. Frontiers in microbiology, 11, 619968.
Flavia Flaviani, Natasha L. Hezelgrave, Tokuwa Kanno, Erica M. Prosdocimi, Evonne Chin-Smith, Alexandra E. Ridout, Djuna K. von Maydell, Vikash Mistry, William G. Wade, Andrew H. Shennan, Konstantina Dimitrakopoulou, Paul T. Seed, A. James Mason, Rachel M. Tribe (2021) Cervicovaginal microbiota and metabolome predict preterm birth risk in an ethnically diverse cohort. JCI Insight. 2021;6(16):e149257
Giorgia Manzo, Federico Gianfanti, Charlotte K. Hind, Leanne Allison, Maria Clarke, Julia Hohenbichler, Ilene Limantoro, Bethany Martin, Phoebe Do Carmo Silva, Philip M. Ferguson, Alice C. Hodgson-Casson, Roland A. Fleck, J. Mark Sutton, David A. Phoenix, and A. James Mason (2021) Impacts of Metabolism and Organic Acids on Cell Wall Composition and Pseudomonas aeruginosa Susceptibility to Membrane Active Antimicrobials. ACS Infectious Diseases 7(8), 2310-2323
Young, J. D., Ma, M. T., Eykyn, T. R., Atkinson, R. A., Abbate, V., Cilibrizzi, A., Hider, R. C., & Blower, P. J. (2021) Dipeptide inhibitors of the prostate specific membrane antigen (PSMA): A comparison of urea and thiourea derivatives. Bioorganic & medicinal chemistry letters, 42, 128044.
Matthew Farleigh, Truc Thuy Pham, Zilin Yu, Jana Kim, Kavitha Sunassee, George Firth, Nafsika Forte, Vijay Chudasama, James R. Baker, Nicholas J. Long, Charlotte Rivas, and Michelle T. Ma (2021) New Bifunctional Chelators Incorporating Dibromomaleimide Groups for Radiolabeling of Antibodies with Positron Emission Tomography Imaging Radioisotopes. Bioconjugate Chemistry 32 (7), 1214-1222
F Dudás, E., Puglisi, R., Korn, S. M., Alfano, C., Bellone, M. L., Piaz, F. D., Kelly, G., Monaca, E., Schlundt, A., Schwalbe, H., & Pastore, A. (2021) Backbone chemical shift spectral assignments of SARS coronavirus-2 non-structural protein nsp9. Journal of Biomolecular NMR assignments, 15(2), 235–241.
Ilkow, V. F., Davies, A. M., Dhaliwal, B., Beavil, A. J., Sutton, B. J., & McDonnell, J. M. (2021) Reviving lost binding sites: Exploring calcium-binding site transitions between human and murine CD23. FEBS open bio, 11(7), 1827–1840.
Colleen Kelly, Nicola Pace, Matthew Gage, Mark Pfuhl (2021) Solution NMR Structure of Titin N2A Region Ig Domain I83 and Its Interaction with Metal Ions. Journal of Molecular Biology 433(13)
Rees, M., Nikoopour, R., Fukuzawa, A. et al. (2021) Making sense of missense variants in TTN-related congenital myopathies. Acta Neuropathol 141, 431–453.
Contact us
Wolfson Wing, Hodgkin Building Guy's Campus King’s College London London, SE1 1UL