Dr Justyna Kozlowska
Franklin-Wilkins Building (5.22B)
150 Stamford Street
London, SE1 9NH
Justyna completed her MRes in Biophysics at King's College London in 2010 and joined the lab as a PhD student funded by a BBSRC CASE studentship awarded to Dr Mason at King's and Dr Michael McArthur at Procarta Biosystems .
Justyna's project investigate the mechanism of action of transcription factor decoys (TFDs) against Escherichia coli. TFDs constitute an exciting new class of antibiotics and are being directed against so called "superbugs".
Justyna developed a multidisciplinary approach using systems biology techniques, in particular NMR metabolomics, to better understand host-bacteria interactions. These include the manipulation of the metabolic environment in the lung by Pseudomonas aeruginosa, one of the major colonisers of the lungs of cystic fibrosis patients, identifying changes in the mouse faecal metabolome as a result of variations in the gut microbiome and, finally, the response of uropathogenic Escherichia coli to challenge with a variety of antimicrobial peptides. The last of these studies enables antibiotic mechanisms of action to be defined from a bacterial perspective. The new methodology is now being applied to TFDs in conjunction with Procarta and Simona Di Blasio.
Having submitted her PhD thesis in September 2014, Justyna worked on a new project in conjunction with Prof Dave Phoenix at LSBU prior to taking up a position at P&G in March 2015.
- Amos, S-B.T.A., Vermeer, L.S., Kozlowska, J., Davy, M., Ferguson, P., Bui, T.T., Drake, A.F., Lorenz, C.D. & Mason, A.J. Antimicrobial peptide potency is facilitated by greater conformational flexibility when binding to Gram-negative bacterial innner membranes. Scientific Reports 2016 (6) 37639
- Rogers, G.B., Kozlowska, J., Keeble, J., Metcalfe, K., Fao, M., Dowd, S.E., Mason, A.J., McGuckin, M.A. & Bruce, K.D. Divergence in gastrointestinal microbiota in physically-separated genetically identical mice. Scientific Reports 2014 (4) 5437
- Kozlowska, J., Vermeer, L.S., Rogers, G.B., Rehnnuma, N., Amos, S-B.T.A., Koller, G., McArthur, M., Bruce, K.D. & Mason, A.J.* Combined systems approaches reveal highly plastic responses to antimicrobial peptide challenge in Escherichia coli. PLoS Pathogens 2014 (10) e1004104
- Kozlowska, J., Rivett, D.W., Vermeer, L.S., Carroll, M.P., Bruce, K.D., Mason, A.J. & Rogers, G.B. A relationship between Pseudomonal growth behaviour and cystic fibrosis patient lung function identified in a metabolomic investigation. Metabolomics2013 (9) 1262-1273
- Vermeer, L.S., Lan, Y., Abbate, V., Ruh, E., Bui, T.T., Wilkinson, L., Kanno, T., Jumagulova, E., Kozlowska, J., Patel, J., McIntyre, C.A., Yam, W.C., Siu, G., Atkinson, R.A., Lam, J.K.W., Bansal, S.S., Drake, A.F., Mitchell, G.H. & Mason, A.J.Conformational flexibility determines selectivity and anti-bacterial, antiplasmodial and anticancer potency of cationic α-helical peptides. J. Biol. Chem. 2012 (287) 34120-34133
- Lan, Y., Yan, Y., Kozlowska, J., Lam, J.K., Drake, A & Mason A.J.* Structural contributions to the intracellular targeting strategy of antimicrobial peptides. Biochim. Biophys. Acta 2010 (1798) 1934-1943