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30 August 2022

Gut and lung organoids open the door to innate immune cell therapies

King’s researchers have found an innovative approach for expanding and maturing innate immune cells in a dish.

Jowlett 3

The finding offers a leap forward for human cell therapeutic strategies that aim to combat chronic inflammatory diseases.

The technique, led by Dr Joana F. Neves at King’s College London, and published today in Cell Reports, uses mini gut and lung organoids to demonstrate, unexpectedly, that it is epithelial cells that drive tissue-specific innate lymphoid cell (ILC) development.

As part of her PhD research at King’s, Dr Geraldine Jowett harnessed this system to show that all ILC subsets can be matured and exponentially expanded in parallel when cultured with these organoids, and that process is dramatically improved when the stroma is depleted, upending gold standard approaches based on immortalised stromal feeder cells.

Dr Jowett, now a Schmidt Science Fellow at Cambridge University, noted how unexpected the human results were: “Our findings in the murine epithelial-only system were extremely robust and reproducible, but early in the project the human stem cell derived intestinal organoid co-cultures could never compete with the efficiency of the murine model. When we developed the mesenchyme-depletion step, we honestly expected that the non-epithelial fraction would outperform the epithelial organoids, as immortalised mesenchymal cells are the gold standard for in vitro ILC research.”

The impact of the epithelial cells really blew us away. We suspected from our murine data that epithelial cells were important for tissue-specific ILC imprints, but we would never have suggested that they were a greater driver of maturation than the intestinal mesenchyme. It’s the kind of finding that makes working with organoids so fun and rewarding.”

Dr Geraldine Jowett

The study forms the basis for a patent application recently filed by King’s College London, which aims to encompass this exciting new approach for driving innate lymphoid cell maturation.

This cell type is enriched in barrier tissues like the gut, lung, and skin, where it regulates homeostasis, rapid response to infection, cancer surveillance, and tissue regeneration. These immune cells are antigen non-specific, but produce large amounts of cytokines, making them an appealing target for cell therapy approaches. However, expanding them outside of the human body in sufficient numbers has proved to be a bottleneck for this application.

Dr Joana F Neves, co-senior author of the study, explains:

We started these studies over 8-years ago, and we were incredible pleased when we observed that the organoid driven expansion of immune cells was so efficient in human organoids. This opens an avenue to perform detailed studies in these rare but important immune cells and has the potential to be used for the expansion of these cells for autologous cell therapy”

Dr Joana F Neves

The authors hope that this human organoid-based system could provide an important advance, not just for our basic understanding of how innate lymphoid cells develop in distal mucosa, but for employing them as a powerful regulator of inflammation.

Read the full journal article Organoids capture tissue-specific innate lymphoid cell development in mice and humans in Cell Reports.

This work was funded by the Wellcome Trust, the European Commission, the UKRI and the NIHR BRC.

In this story

Joana Neves

Senior Lecturer in Mucosal Immunology and Group Leader

Research Associate

Diana Coman

Research Associate

Rita Antunes dos Reis

Research Assistant

Tung-Jui  Trieu

Research Associate

Davide Danovi

Senior Lecturer

Eileen Gentleman

Professor in Bioengineering

Mike Curtis

Professor of Microbiology