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The Nikon Imaging Centre at King’s College London is a Core Facility for light microscopy, developed and operated as a partnership between King’s College London and Nikon Instruments UK.

This partnership allows us to offer the latest state-of-the-art light microscopy and imaging to the research community and the Centre serves as a learning platform for our regional corporate partners and contributors.

Our mission is to promote innovation across a broad range of biological research fields by providing access to cutting edge microscopy and imaging equipment.

Before requesting access to the Centre, please review the access policy and follow the steps outlined in the 'access' tab below to request an account for the online scheduling system and to arrange training.  

We provide comprehensive training in basic and advanced light microscopy techniques and access to a broad range of Nikon microscopes from widefield to super-resolution. Nikon Imaging Centre staff support users on site and the Centre has close links to Advanced Imaging Specialists and Engineers at Nikon. We offer technical consultation and ongoing support to the research community at King’s College London and beyond to ensure researchers achieve high-quality data outputs.

We contribute to teaching and education at King’s College London as well as hosting instrument demos and workshops in partnership with Nikon for the benefit of the local imaging community.

Facility staff

MicrosoftTeams-image

Manager – Nikon Imaging Centre

Vincenzo Infante

Nikon Imaging Technician

RES_Chantal Hubens

Nikon Imaging Centre, Deputy Manager

Technician - Nikon Imaging Centre

Affiliated academics

maddy-parsons

Associate Dean for Impact & Innovation

Juan Martin-Serrano

Professor of Viral Cell Biology

aleksandar-ivetic

Reader in Cardiovascular Biology

DS thumbnail540

Professor of Molecular Neuroscience

Uwe Drescher

Professor for Molecular Neuroscience

Jeremy Carlton new 160x160

Wellcome Trust Senior Research Fellow

Lecturer in Old Age Psychiatry

George Chennell (002)

Wohl Cellular Imaging Centre Manager

Advanced Imaging Manager at Nikon Instruments

General Manager - (Healthcare Division) at Nikon Instruments

Before requesting access to the facility, please view the access policy. Contact the Facility Manager to discuss your requirements to ensure your project is costed accurately​​​​​​​.

Related equipment

NIC Confocal A1 image
A1R confocal with spectral detector

Resonant scanning - The A1R+ confocal has a hybrid scan-head that incorporates Nikon's HD resonant scanner in addition to a galvonometer scanner

NIC banner 3
A1 inverted confocal with spectral detector

High resolution imaging - The A1+ utilizes a galvano scanner which enables high-resolution imaging of up to 4096 x 4096 pixels (512 x 512 pixels also possible).

NIC Upright Confocal image
A1 upright confocal

High resolution imaging - The A1+ utilizes a galvano scanner which enables high-resolution imaging of up to 4096 x 4096 pixels (512 x 512 pixels also possible).

NIC Ti2 image
Ti2 wide-field imaging systems

Motorisation - Wide-field 1 and 2 use Nikon's Ti2 inverted microscopes which are fully motorised for multi-dimensional imaging.

NSTORM
N-STORM super resolution

Super resolution - Imaging at lateral resolution of approximately 20nm, and axial of 50nm can be achieved.

Inverted spinning disk confocal
Inverted spinning disk confocal

Fast confocal - Equipped with a Yokogawa CSU-X1 spinning disk unit for the ultimate in high speed and low phototoxicity for live cell imaging.

NIC SoRa Image
SoRa - Spinning Disk Super-Resolution by Optical Pixel Reassignment

Fast confocal and super-resolution - equipped with a Yokogawa CSU-W1 dual disk scan head. High-speed low phototoxicity confocal imaging possible.

Selected publications

2023

Chandler, J., Treacy, C., Ameer-Beg, S., and Kampourakis, T. (2023), In situ FRET-based localization of the N terminus of myosin binding protein-C in heart muscle cells, PNS.2023. 10.1073.

Skourti, E., Volpe, A., Lang, C., Johnson, P., Panagaki, F., Fruhwirth, G.O. (2023), Spatiotemporal quantitative microRNA-155 imaging reports immune-mediated changes in a triple-negative breast cancer model, Frontiers in Immunology IF 9.4.2023.10.3389.

Crescioli, S., Correa, I., Ng, J., Willsmore, Z.N., Laddach, R., Chenoweth, A., Chauhan, J., Di Meo, A., Stewart, A., Kalliolia, E., Alberts, E., Adams, R., Harris, R.J., Mele, S., Pellizzari, G., Black, A.B.M., Bax, H.J., Cheung, A., Nakamura, M., Hoffmann, R.M., Terranova-Barberio, M., Ali, N., Batruch, I., Soosaipillai, A., Karagiannis, S.N. (2023),B cell profiles, antibody repertoire and reactivity reveal dysregulated responses with autoimmune features in melanoma, Nature communications IF 16.6.2023.10.1038.

Fanelli, G., Romano, M., Lombardi, G. and Sacks, S.H. (2023), Soluble Collection 11 (CL-11) Acts as an Immunosuppressive Molecule Potentially Used by Stem Cell-Derived Retinal Epithelial Cells to Modulate T Cell Response, Cells. 2023.180510.3390.

Culley, S., Cuber Caballero, A., Burden, J.J., Uhlmann, V. (2023), Made to measure: An introduction to quantifying microscopy data in the life sciences, Journal of Microscopy IF2. 2023.10.1111.

Alkattan, R., Ajaj, R., Koller, G., Banerji, S., Deb, S. (2023), A self-etch bonding system with potential to eliminate selective etching and resist proteolytic degradation, Journal of Dentistry.2023.10.1016.

Perez-Ortiz, G., Sidda, J.D., Peate, J., Ciccarelli, D., Ding, Y., Barry, S.M. (2023), Production of copropophyrin III, biliverdin and bilirubin by the rufomycin producer, Streptomyces atratus, Front. Microbiol. 2023.10.3389.

2022

Glebov, O., Williamson, D., Owen, D.M., Hortobágyi, T., Troakes, C., and Aarsland, D. (2022) Structural synaptic signatures of Alzheimer's disease and dementia with Lewy bodies in the male brain, l.IF5.2022.10.1111. 

Bourke, S., Donà, F., Teijeiro Gonzalez, Y., Qazi Chaudhry, B., Panamarova, M., Mackay, E., Zammit, P.S., Dailey, L.A., Eggert, U.S., Suhling, K., and Green, M.A. (2022), Biocompatible Magnetic Conjugated Polymer Nanoparticles for Optical and Lifetime Imaging Applications in the First Biological Window, ACS Publications IF 5.7. 2022.10.1021.

Donà, F., Özbalci, C., Paquola, A., Ferrentino, F., Terry, S.J., Storck, E.M., Wang, G., and Eggert, U.S. (2022), Removal of Stomatin, a Membrane-Associated Cell Division Protein, Results in Specific Cellular Lipid Changes, Journal of the American Chemical Society.2022. 10.1021/jacs.2c07907.

Ranu, N., Laitila, J., Dugdale, H.F., Mariano, J., Kolb, J.S., Wallgren-Pettersson, C., Witting, N., Vissing, J., Vilchez, J.J., Fiorillo, C., Zanoteli, E., Auranen, M., Jokela, M., Tasca, G., Claeys, K.G., Voermans, N.C, Palmio, J., Huovinen, S., Moggio, M., Nyegaard Beck, T., Kontrogianni-Konstantopoulos, A., Granzier, H. & Ochala, J. (2022), NEB mutations disrupt the super-relaxed state of myosin and remodel the muscle metabolic proteome in nemaline myopathy, Acta Neuropathologica Communications.2022. 10.1186.

Louis, B., Tewary, M., Bremer, A.W., Philippeos, C., Negri, V.A., Zijl, S, Gartner, Z.J., Schaffer, D.V., Watt, F.M. (2022), A reductionist approach to determine the effect of cell-cell contact on human epidermal stem cell differentiation, Acta Biomaterialia IF 9.7. 2022. 10.1016.

Parsons, R.B., Kocinaj, A., Ruiz Pulido, G., Prendergast, S.A., Parsons, A.E., Facey, P.D., Hirth, F. (2022), Alpha-synucleinopathy reduces NMNAT3 protein levels and neurite formation that can be rescued by targeting the NAD+ pathway, Human Molecular Genetics. 2022.10.1093.

2021

Fadul, J., Zulueta-Coarasa, T., Slattum, G.M. et al. (2021) KRas-transformed epithelia cells invade and partially dedifferentiate by basal cell extrusion. Nat Comm; 12, 7180.

Nikolaos Ioannou, Patrick R. Hagner, Matt Stokes, Anita K. Gandhi, Benedetta Apollonio, Mariam Fanous, Despoina Papazoglou, Lesley-Ann Sutton, Richard Rosenquist, Rose-Marie Amini, Hsiling Chiu, Antonia Lopez-Girona, Preethi Janardhanan, Farrukh T. Awan, Jeffrey Jones, Neil E. Kay, Tait D. Shanafelt, Martin S. Tallman, Kostas Stamatopoulos, Piers E. M. Patten, Anna Vardi, Alan G. Ramsay. (2021) Triggering interferon signaling in T cells with avadomide sensitizes CLL to anti-PD-L1/PD-1 immunotherapy.; 137 (2): 216–231.

Sioned F. Jones, Himanshu Joshi, Stephen J. Terry, Jonathan R. Burns, Aleksei Aksimentiev, Ulrike S. Eggert, and Stefan Howorka. (2021). Hydrophobic Interactions between DNA Duplexes and Synthetic and Biological Membranes. J. Am. Chem. Soc.; 143 (22), 8305-8313.

2020

Woods S, O'Brien LM, Butcher W, et al. (2020) Glucosamine-NISV delivers antibody across the blood-brain barrier: Optimization for treatment of encephalitic viruses. J Control Release; 324: 644-656.

Michael J. Shannon, Judith Pineau, Juliette Griffié, Jesse Aaron, Tamlyn Peel, David J. Williamson, Rose Zamoyska, Andrew P. Cope, Georgina H. Cornish, Dylan M. Owen, Ana-Maria Lennon-Duménil. (2020). Differential nanoscale organisation of LFA-1 modulates T-cell migration. J Cell Sci; 133 (5): jcs232991.

2019

Negri VA, Logtenberg MEW, Renz LM, Oules B, Walko G, Watt FM. (2019). Delta-like 1-mediated cis-inhibition of Jagged1/2 signalling inhibits differentiation of human epidermal cells in culture. Sci Rep. 9 (1):10825.

Morton PE, Perrin C, Levitt J, et al. (2019). TNFR1 membrane reorganization promotes distinct modes of TNFα signaling. Sci Signal. 12(592):eaaw2418.

Ross JA, Levy Y, Ripolone M, et al (2019). Impairments in contractility and cytoskeletal organisation cause nuclear defects in nemaline myopathy. Acta Neuropathol;138(3):477-495.

Evans R, Flores-Borja F, Nassiri S, et al. (2019). Integrin-Mediated Macrophage Adhesion Promotes Lymphovascular Dissemination in Breast Cancer. Cell Rep; 27(7):1967-1978.e4.

Ventimiglia LN, Cuesta-Geijo MA, Martinelli N, et al (2018). CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope. Dev Cell. 47 (5):547-563.e6.

Doyle T, Moncorgé O, Bonaventure B, et al. (2018). The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry. Nat Microbiol., 4(3):539].

2018

Sadler JBA, Wenzel DM, Williams LK, et al. (2018). A cancer-associated polymorphism in ESCRT-III disrupts the abscission checkpoint and promotes genome instability. Proc Natl Acad Sci U S A. 115(38):E8900-E8908.

Michael M, Begum R, Chan GK, et al. (2019). Kindlin-1 Regulates Epidermal Growth Factor Receptor Signaling. J Invest Dermatol;139(2):369-379.

Rognoni E, Pisco AO, Hiratsuka T, et al. (2018). Fibroblast state switching orchestrates dermal maturation and wound healing. Mol Syst Biol.14(8):e8174.

Cheung A, Opzoomer J, Ilieva KM, et al. (2018). Anti-Folate Receptor Alpha-Directed Antibody Therapies Restrict the Growth of Triple-negative Breast Cancer. Clin Cancer Res. 24(20):5098-5111.

Monypenny J, Milewicz H, Flores-Borja F, et al. (2018). ALIX Regulates Tumor-Mediated Immunosuppression by Controlling EGFR Activity and PD-L1 Presentation. Cell Rep. 24(3):630-641.

Marsh R.J, Pfisterer K, Bennett P, Hirvonen LM, Gautel M, Jones GE, Cox S. (2018). Artifact-free high-density localization microscopy analysis. Nature Methods.

Peters R, Griffié J, Burn GL, Williamson DJ, Owen DM. (2018). Quantitative fibre analysis of single-molecule localization microscopy data. Scientific Reports. 8; 10418.

2017

Ashdown GW, Burn GL, Williamson DJ, Pandžić E, Peters R, Holden M, Ewers H, Shao L, Wiseman PW, Owen DM. (2017). Live-Cell Super-resolution Reveals F-Actin and Plasma Membrane Dynamics at the T Cell Synapse. Biophysical Journal. 112;1703–1713.

2016

Starling GP, Yip YY, Sanger A, Morton PE, Eden ER, Dodding MP. (2016). Folliculin directs the formation of a Rab34–RILP complex to control the nutrient‐dependent dynamic distribution of lysosomes. EMBO Reports. 17; 823-841

Deans PJM, Raval P, Sellers KJ, Gatford NJF, Halai S, Duarte RRR, Shum C, Warre-Cornish K, Kaplun VE, Cocks G, Hill M, Bray NJ, Price J, Srivastava DP. (2016). Psychosis risk candidate ZNF804A localizes to synapses and regulates neurite formation and dendritic spine structure. Biological Psychiatry

Olmos Y, Perdrix-Rosell A, Carlton JG. (2016). Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation. Current Biology.

Jayo A, Malboubi M, Antoku S, Chang W, Ortiz-Zapater E, Groen C, Pfisterer K, Tootle T, Charras G, Gundersen GG, Parsons M. (2016) Regulates Nuclear Movement and Deformation in Migrating Cells. Developmental Cell. 38(4):371-383.

Arbore G, West EE, Spolski R, Robertson AAB, Klos A, Rheinheimer C, Dutow P, Woodruff TM, Yu ZX, O’Neill LA, Coll RC, Sher A, Leonard WJ, Köhl J, Monk P, Cooper MA, Arno M, Afzali B, Lachmann HJ, Cope AP, Mayer-Barber KD, Kemper C. (2016). T helper 1 immunity requires complement-driven NLRP3 inflammasome activity in CD4+ T cells. Science. 352; 6292.

2015

Villari G, Jayo A, Zanet J, Fitch B, Serrels B, Frame M, Stramer B, Goult BT, Parsons M. (2015). A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration. J Cell Sci. 128(24):4601-14

2014

Ashdown GW, Cope A, Wiseman PW, Owen DM. (2014). Molecular Flow Quantified beyond the Diffraction Limit by Spatiotemporal Image Correlation of Structured Illumination Microscopy Data. Biophysical Journal, 107(9), L21–L23.

Marra P, Mathew S, Grigoriadis A, Wu Y, Kyle-Cezar F, Watkins J, Rashid M, De Rinaldis E, Hessey S, Gazinska P, Hayday A, Tutt A. (2014). IL15RA Drives Antagonistic Mechanisms of Cancer Development and Immune Control in Lymphocyte-Enriched Triple-Negative Breast Cancers. Cancer Research.74(17)

2013

Stürzenbaum SR, Höckner M, Panneerselvam A, Levitt J, Bouillard JS, Taniguchi S, Dailey LA, Ahmad Khanbeigi R, Rosca EV, Thanou M et al. (2013). Biosynthesis of luminescent quantum dots in an earthworm. Nature Nanotechnology 8, 57–60.

Hng KI, Dormann D. (2013). ConfocalCheck - A Software Tool for the Automated Monitoring of Confocal Microscope Performance. PLoS ONE 8, e79879.

Asgari, E., Le Friec, G., Yamamoto, H., Perucha, E., Sacks, S.S., Köhl, J., Cook, H.T., and Kemper, C. (2013). C3a modulates IL-1β secretion in human monocytes by regulating ATP efflux and subsequent NLRP3 inflammasome activation. Blood. 122(20):3473-81

Before requesting access to the NIC@King’s, please review the access policy. King's students and staff can view access rates on the Research Facilities SharePoint site. 

 To gain access to the Centre you will need to fill out two forms:

  1.  An account request for our online scheduling system. If you are a King's student or staff member, you must enter a grant code (to be charged for your usage) in the box that asks for an 'Account number'. If you are from an institution other than King's, please contact a member of the NIC team for a consultation.
  2. A training request form, submitted through the online scheduling system. This will allow you to provide details of the samples you want to image and the instrument you are interested in using. You should submit a separate form for each instrument on which you require training.

If you are not sure which microscope you need for your work and would prefer a consultation please contact a member of NIC@King's team.

You will need to complete two training sessions before you can use the microscopes unassisted. At the first session, we will fully demonstrate the microscope and make configurations appropriate to your needs. (You are encouraged to bring samples with you to this training sessions). During the second training session, you will control the microscope under supervision of a member of the NIC team. Once you are confident operating the microscope independently, you will be given access to the booking schedule for the microscope on which you completed the two training sessions. Initially this access will be limited to office hours (9am-5pm), to ensure a member of the NIC team is present. Once the team are confident you can use the use the microscope unaided, you will be granted out-of-hours access. This does not happen automatically, please contact a member of the team to request out-of-hours access.

All subsequent requests for training should be made through our online scheduling system.

If you have trouble with any of the forms, please contact the Facility Manager james.levitt@kcl.ac.uk.

 

We provide comprehensive training in basic and advanced light microscopy techniques and access to a broad range of Nikon microscopes from widefield to super-resolution. Nikon Imaging Centre staff support users on site and the Centre has close links to Advanced Imaging Specialists and Engineers at Nikon. We offer technical consultation and ongoing support to the research community at King’s College London and beyond to ensure researchers achieve high-quality data outputs.

We contribute to teaching and education at King’s College London as well as hosting instrument demos and workshops in partnership with Nikon for the benefit of the local imaging community.

Facility staff

MicrosoftTeams-image

Manager – Nikon Imaging Centre

Vincenzo Infante

Nikon Imaging Technician

RES_Chantal Hubens

Nikon Imaging Centre, Deputy Manager

Technician - Nikon Imaging Centre

Affiliated academics

maddy-parsons

Associate Dean for Impact & Innovation

Juan Martin-Serrano

Professor of Viral Cell Biology

aleksandar-ivetic

Reader in Cardiovascular Biology

DS thumbnail540

Professor of Molecular Neuroscience

Uwe Drescher

Professor for Molecular Neuroscience

Jeremy Carlton new 160x160

Wellcome Trust Senior Research Fellow

Lecturer in Old Age Psychiatry

George Chennell (002)

Wohl Cellular Imaging Centre Manager

Advanced Imaging Manager at Nikon Instruments

General Manager - (Healthcare Division) at Nikon Instruments

Before requesting access to the facility, please view the access policy. Contact the Facility Manager to discuss your requirements to ensure your project is costed accurately​​​​​​​.

Related equipment

NIC Confocal A1 image
A1R confocal with spectral detector

Resonant scanning - The A1R+ confocal has a hybrid scan-head that incorporates Nikon's HD resonant scanner in addition to a galvonometer scanner

NIC banner 3
A1 inverted confocal with spectral detector

High resolution imaging - The A1+ utilizes a galvano scanner which enables high-resolution imaging of up to 4096 x 4096 pixels (512 x 512 pixels also possible).

NIC Upright Confocal image
A1 upright confocal

High resolution imaging - The A1+ utilizes a galvano scanner which enables high-resolution imaging of up to 4096 x 4096 pixels (512 x 512 pixels also possible).

NIC Ti2 image
Ti2 wide-field imaging systems

Motorisation - Wide-field 1 and 2 use Nikon's Ti2 inverted microscopes which are fully motorised for multi-dimensional imaging.

NSTORM
N-STORM super resolution

Super resolution - Imaging at lateral resolution of approximately 20nm, and axial of 50nm can be achieved.

Inverted spinning disk confocal
Inverted spinning disk confocal

Fast confocal - Equipped with a Yokogawa CSU-X1 spinning disk unit for the ultimate in high speed and low phototoxicity for live cell imaging.

NIC SoRa Image
SoRa - Spinning Disk Super-Resolution by Optical Pixel Reassignment

Fast confocal and super-resolution - equipped with a Yokogawa CSU-W1 dual disk scan head. High-speed low phototoxicity confocal imaging possible.

Selected publications

2023

Chandler, J., Treacy, C., Ameer-Beg, S., and Kampourakis, T. (2023), In situ FRET-based localization of the N terminus of myosin binding protein-C in heart muscle cells, PNS.2023. 10.1073.

Skourti, E., Volpe, A., Lang, C., Johnson, P., Panagaki, F., Fruhwirth, G.O. (2023), Spatiotemporal quantitative microRNA-155 imaging reports immune-mediated changes in a triple-negative breast cancer model, Frontiers in Immunology IF 9.4.2023.10.3389.

Crescioli, S., Correa, I., Ng, J., Willsmore, Z.N., Laddach, R., Chenoweth, A., Chauhan, J., Di Meo, A., Stewart, A., Kalliolia, E., Alberts, E., Adams, R., Harris, R.J., Mele, S., Pellizzari, G., Black, A.B.M., Bax, H.J., Cheung, A., Nakamura, M., Hoffmann, R.M., Terranova-Barberio, M., Ali, N., Batruch, I., Soosaipillai, A., Karagiannis, S.N. (2023),B cell profiles, antibody repertoire and reactivity reveal dysregulated responses with autoimmune features in melanoma, Nature communications IF 16.6.2023.10.1038.

Fanelli, G., Romano, M., Lombardi, G. and Sacks, S.H. (2023), Soluble Collection 11 (CL-11) Acts as an Immunosuppressive Molecule Potentially Used by Stem Cell-Derived Retinal Epithelial Cells to Modulate T Cell Response, Cells. 2023.180510.3390.

Culley, S., Cuber Caballero, A., Burden, J.J., Uhlmann, V. (2023), Made to measure: An introduction to quantifying microscopy data in the life sciences, Journal of Microscopy IF2. 2023.10.1111.

Alkattan, R., Ajaj, R., Koller, G., Banerji, S., Deb, S. (2023), A self-etch bonding system with potential to eliminate selective etching and resist proteolytic degradation, Journal of Dentistry.2023.10.1016.

Perez-Ortiz, G., Sidda, J.D., Peate, J., Ciccarelli, D., Ding, Y., Barry, S.M. (2023), Production of copropophyrin III, biliverdin and bilirubin by the rufomycin producer, Streptomyces atratus, Front. Microbiol. 2023.10.3389.

2022

Glebov, O., Williamson, D., Owen, D.M., Hortobágyi, T., Troakes, C., and Aarsland, D. (2022) Structural synaptic signatures of Alzheimer's disease and dementia with Lewy bodies in the male brain, l.IF5.2022.10.1111. 

Bourke, S., Donà, F., Teijeiro Gonzalez, Y., Qazi Chaudhry, B., Panamarova, M., Mackay, E., Zammit, P.S., Dailey, L.A., Eggert, U.S., Suhling, K., and Green, M.A. (2022), Biocompatible Magnetic Conjugated Polymer Nanoparticles for Optical and Lifetime Imaging Applications in the First Biological Window, ACS Publications IF 5.7. 2022.10.1021.

Donà, F., Özbalci, C., Paquola, A., Ferrentino, F., Terry, S.J., Storck, E.M., Wang, G., and Eggert, U.S. (2022), Removal of Stomatin, a Membrane-Associated Cell Division Protein, Results in Specific Cellular Lipid Changes, Journal of the American Chemical Society.2022. 10.1021/jacs.2c07907.

Ranu, N., Laitila, J., Dugdale, H.F., Mariano, J., Kolb, J.S., Wallgren-Pettersson, C., Witting, N., Vissing, J., Vilchez, J.J., Fiorillo, C., Zanoteli, E., Auranen, M., Jokela, M., Tasca, G., Claeys, K.G., Voermans, N.C, Palmio, J., Huovinen, S., Moggio, M., Nyegaard Beck, T., Kontrogianni-Konstantopoulos, A., Granzier, H. & Ochala, J. (2022), NEB mutations disrupt the super-relaxed state of myosin and remodel the muscle metabolic proteome in nemaline myopathy, Acta Neuropathologica Communications.2022. 10.1186.

Louis, B., Tewary, M., Bremer, A.W., Philippeos, C., Negri, V.A., Zijl, S, Gartner, Z.J., Schaffer, D.V., Watt, F.M. (2022), A reductionist approach to determine the effect of cell-cell contact on human epidermal stem cell differentiation, Acta Biomaterialia IF 9.7. 2022. 10.1016.

Parsons, R.B., Kocinaj, A., Ruiz Pulido, G., Prendergast, S.A., Parsons, A.E., Facey, P.D., Hirth, F. (2022), Alpha-synucleinopathy reduces NMNAT3 protein levels and neurite formation that can be rescued by targeting the NAD+ pathway, Human Molecular Genetics. 2022.10.1093.

2021

Fadul, J., Zulueta-Coarasa, T., Slattum, G.M. et al. (2021) KRas-transformed epithelia cells invade and partially dedifferentiate by basal cell extrusion. Nat Comm; 12, 7180.

Nikolaos Ioannou, Patrick R. Hagner, Matt Stokes, Anita K. Gandhi, Benedetta Apollonio, Mariam Fanous, Despoina Papazoglou, Lesley-Ann Sutton, Richard Rosenquist, Rose-Marie Amini, Hsiling Chiu, Antonia Lopez-Girona, Preethi Janardhanan, Farrukh T. Awan, Jeffrey Jones, Neil E. Kay, Tait D. Shanafelt, Martin S. Tallman, Kostas Stamatopoulos, Piers E. M. Patten, Anna Vardi, Alan G. Ramsay. (2021) Triggering interferon signaling in T cells with avadomide sensitizes CLL to anti-PD-L1/PD-1 immunotherapy.; 137 (2): 216–231.

Sioned F. Jones, Himanshu Joshi, Stephen J. Terry, Jonathan R. Burns, Aleksei Aksimentiev, Ulrike S. Eggert, and Stefan Howorka. (2021). Hydrophobic Interactions between DNA Duplexes and Synthetic and Biological Membranes. J. Am. Chem. Soc.; 143 (22), 8305-8313.

2020

Woods S, O'Brien LM, Butcher W, et al. (2020) Glucosamine-NISV delivers antibody across the blood-brain barrier: Optimization for treatment of encephalitic viruses. J Control Release; 324: 644-656.

Michael J. Shannon, Judith Pineau, Juliette Griffié, Jesse Aaron, Tamlyn Peel, David J. Williamson, Rose Zamoyska, Andrew P. Cope, Georgina H. Cornish, Dylan M. Owen, Ana-Maria Lennon-Duménil. (2020). Differential nanoscale organisation of LFA-1 modulates T-cell migration. J Cell Sci; 133 (5): jcs232991.

2019

Negri VA, Logtenberg MEW, Renz LM, Oules B, Walko G, Watt FM. (2019). Delta-like 1-mediated cis-inhibition of Jagged1/2 signalling inhibits differentiation of human epidermal cells in culture. Sci Rep. 9 (1):10825.

Morton PE, Perrin C, Levitt J, et al. (2019). TNFR1 membrane reorganization promotes distinct modes of TNFα signaling. Sci Signal. 12(592):eaaw2418.

Ross JA, Levy Y, Ripolone M, et al (2019). Impairments in contractility and cytoskeletal organisation cause nuclear defects in nemaline myopathy. Acta Neuropathol;138(3):477-495.

Evans R, Flores-Borja F, Nassiri S, et al. (2019). Integrin-Mediated Macrophage Adhesion Promotes Lymphovascular Dissemination in Breast Cancer. Cell Rep; 27(7):1967-1978.e4.

Ventimiglia LN, Cuesta-Geijo MA, Martinelli N, et al (2018). CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope. Dev Cell. 47 (5):547-563.e6.

Doyle T, Moncorgé O, Bonaventure B, et al. (2018). The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry. Nat Microbiol., 4(3):539].

2018

Sadler JBA, Wenzel DM, Williams LK, et al. (2018). A cancer-associated polymorphism in ESCRT-III disrupts the abscission checkpoint and promotes genome instability. Proc Natl Acad Sci U S A. 115(38):E8900-E8908.

Michael M, Begum R, Chan GK, et al. (2019). Kindlin-1 Regulates Epidermal Growth Factor Receptor Signaling. J Invest Dermatol;139(2):369-379.

Rognoni E, Pisco AO, Hiratsuka T, et al. (2018). Fibroblast state switching orchestrates dermal maturation and wound healing. Mol Syst Biol.14(8):e8174.

Cheung A, Opzoomer J, Ilieva KM, et al. (2018). Anti-Folate Receptor Alpha-Directed Antibody Therapies Restrict the Growth of Triple-negative Breast Cancer. Clin Cancer Res. 24(20):5098-5111.

Monypenny J, Milewicz H, Flores-Borja F, et al. (2018). ALIX Regulates Tumor-Mediated Immunosuppression by Controlling EGFR Activity and PD-L1 Presentation. Cell Rep. 24(3):630-641.

Marsh R.J, Pfisterer K, Bennett P, Hirvonen LM, Gautel M, Jones GE, Cox S. (2018). Artifact-free high-density localization microscopy analysis. Nature Methods.

Peters R, Griffié J, Burn GL, Williamson DJ, Owen DM. (2018). Quantitative fibre analysis of single-molecule localization microscopy data. Scientific Reports. 8; 10418.

2017

Ashdown GW, Burn GL, Williamson DJ, Pandžić E, Peters R, Holden M, Ewers H, Shao L, Wiseman PW, Owen DM. (2017). Live-Cell Super-resolution Reveals F-Actin and Plasma Membrane Dynamics at the T Cell Synapse. Biophysical Journal. 112;1703–1713.

2016

Starling GP, Yip YY, Sanger A, Morton PE, Eden ER, Dodding MP. (2016). Folliculin directs the formation of a Rab34–RILP complex to control the nutrient‐dependent dynamic distribution of lysosomes. EMBO Reports. 17; 823-841

Deans PJM, Raval P, Sellers KJ, Gatford NJF, Halai S, Duarte RRR, Shum C, Warre-Cornish K, Kaplun VE, Cocks G, Hill M, Bray NJ, Price J, Srivastava DP. (2016). Psychosis risk candidate ZNF804A localizes to synapses and regulates neurite formation and dendritic spine structure. Biological Psychiatry

Olmos Y, Perdrix-Rosell A, Carlton JG. (2016). Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation. Current Biology.

Jayo A, Malboubi M, Antoku S, Chang W, Ortiz-Zapater E, Groen C, Pfisterer K, Tootle T, Charras G, Gundersen GG, Parsons M. (2016) Regulates Nuclear Movement and Deformation in Migrating Cells. Developmental Cell. 38(4):371-383.

Arbore G, West EE, Spolski R, Robertson AAB, Klos A, Rheinheimer C, Dutow P, Woodruff TM, Yu ZX, O’Neill LA, Coll RC, Sher A, Leonard WJ, Köhl J, Monk P, Cooper MA, Arno M, Afzali B, Lachmann HJ, Cope AP, Mayer-Barber KD, Kemper C. (2016). T helper 1 immunity requires complement-driven NLRP3 inflammasome activity in CD4+ T cells. Science. 352; 6292.

2015

Villari G, Jayo A, Zanet J, Fitch B, Serrels B, Frame M, Stramer B, Goult BT, Parsons M. (2015). A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration. J Cell Sci. 128(24):4601-14

2014

Ashdown GW, Cope A, Wiseman PW, Owen DM. (2014). Molecular Flow Quantified beyond the Diffraction Limit by Spatiotemporal Image Correlation of Structured Illumination Microscopy Data. Biophysical Journal, 107(9), L21–L23.

Marra P, Mathew S, Grigoriadis A, Wu Y, Kyle-Cezar F, Watkins J, Rashid M, De Rinaldis E, Hessey S, Gazinska P, Hayday A, Tutt A. (2014). IL15RA Drives Antagonistic Mechanisms of Cancer Development and Immune Control in Lymphocyte-Enriched Triple-Negative Breast Cancers. Cancer Research.74(17)

2013

Stürzenbaum SR, Höckner M, Panneerselvam A, Levitt J, Bouillard JS, Taniguchi S, Dailey LA, Ahmad Khanbeigi R, Rosca EV, Thanou M et al. (2013). Biosynthesis of luminescent quantum dots in an earthworm. Nature Nanotechnology 8, 57–60.

Hng KI, Dormann D. (2013). ConfocalCheck - A Software Tool for the Automated Monitoring of Confocal Microscope Performance. PLoS ONE 8, e79879.

Asgari, E., Le Friec, G., Yamamoto, H., Perucha, E., Sacks, S.S., Köhl, J., Cook, H.T., and Kemper, C. (2013). C3a modulates IL-1β secretion in human monocytes by regulating ATP efflux and subsequent NLRP3 inflammasome activation. Blood. 122(20):3473-81

Before requesting access to the NIC@King’s, please review the access policy. King's students and staff can view access rates on the Research Facilities SharePoint site. 

 To gain access to the Centre you will need to fill out two forms:

  1.  An account request for our online scheduling system. If you are a King's student or staff member, you must enter a grant code (to be charged for your usage) in the box that asks for an 'Account number'. If you are from an institution other than King's, please contact a member of the NIC team for a consultation.
  2. A training request form, submitted through the online scheduling system. This will allow you to provide details of the samples you want to image and the instrument you are interested in using. You should submit a separate form for each instrument on which you require training.

If you are not sure which microscope you need for your work and would prefer a consultation please contact a member of NIC@King's team.

You will need to complete two training sessions before you can use the microscopes unassisted. At the first session, we will fully demonstrate the microscope and make configurations appropriate to your needs. (You are encouraged to bring samples with you to this training sessions). During the second training session, you will control the microscope under supervision of a member of the NIC team. Once you are confident operating the microscope independently, you will be given access to the booking schedule for the microscope on which you completed the two training sessions. Initially this access will be limited to office hours (9am-5pm), to ensure a member of the NIC team is present. Once the team are confident you can use the use the microscope unaided, you will be granted out-of-hours access. This does not happen automatically, please contact a member of the team to request out-of-hours access.

All subsequent requests for training should be made through our online scheduling system.

If you have trouble with any of the forms, please contact the Facility Manager james.levitt@kcl.ac.uk.

 


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