The Centre for Ultrastructural Imaging (CUI) is the central electron microscopy facility at King’s College London. We provide a state-of-the-art facility to internal and external collaborators from academic, commercial, and industrial sectors across medicine, biological sciences, chemistry, physics, materials sciences, and engineering.
Our vision is to deliver expert electron microscopy services through a collaborative research environment, offering support in experimental design, advanced imaging techniques, data interpretation, and training.
We provide cutting-edge and comprehensive workflows to support life and materials science electron microscopy.
We work with leading international research groups, as well as instrument manufacturers to support advanced electron microscopy techniques and promote novel ideas and innovation.
Techniques available in the CUI.
- Transmission Electron Microscopy (TEM)High-resolution images of nanoscale structures within cells and tissues.
- Scanning Electron Microscopy (SEM)Using SEM, we are able to generate an image showing topographical detail.
- Critical Point DryingCritical point drying preserves ultrastructure and provide a room temperature stable sample for imaging in a scanning electron microscope.
- High-pressure freezing and freeze substitutionHPF is a cryo-fixation method that allows the study of biological specimens with improved preservation of cellular structures.
- Energy Dispersive X-ray Spectroscopy (EDS)Energy Dispersive x-ray Spectroscopy is used to determine and quantify the elemental composition of a sample.
- Electron Tomography (ET)ET is a powerful technique to generate a detailed 3D volume which reveals structures of sub-cellular macro-molecular objects at the nanometre scale.
- Scanning Transmission Electron Microscopy (STEM)A scanning transmission electron microscope is equipped with additional scanning coils, detectors and necessary circuitry.
- Serial Block Face Scanning Electron Microscopy (SBF-SEM)SBF-SEM an automated 3D imaging technique that combines in situ ultramicrotomy with scanning electron microscopy.
- Correlative Light and Electron Microscopy (CLEM)CLEM is an imaging technique that combines fluorescence light microscopy (FM) and electron microscopy (EM) to analyse the same biological sample.
- Plunge FreezingPlunge freezing is a cryo-fixation technique used to fix delicate samples for cryo-Electron Microscopy.
Facility staff
Facility Manager - Centre for Ultrastructural Imaging
Electron Microscopy Applications Specialist
Electron Microscopy Technician
Affiliated academics
Associate Director Innovation & Business Development – Imaging Core Facilities and Smart Trials Hub
Business Systems and Engagement Coordinator
Equipment available at the CUI
Related equipment
JEOL JEM-1400Flash Transmission Electron Microscope (TEM)
The JEOL JEM-1400Flash is used to study a variety of materials from nanoparticles, viruses, bacteria, and sections of cells and tissues.
JEOL JEM-F200 Transmission Electron Microscope (TEM)
The JEOL JEM-F200 is our 200Kv Transmission Electron Microscope for advanced room temperature applications such as electron tomography.
JEOL JSM 7800F Prime Scanning Electron Microscope (SEM)
The JEOL JSM 7800F Prime SEM is our current top end SEM, equipped with a Schottky Field Emission Gun.
JEOL JSM 7800F Serial Block Face-Scanning Electron Microscope (SBF-SEM)
The JSM 7800F Serial Block Face SEM is equipped with a ConnectomX Katana microtome for volume EM.
JEOL JCM 7000 NeoScope Scanning Electron Microscope (SEM)
The JEOL JCM 7000 NeoScope is our benchtop scanning electron microscope (SEM). A small size, user friendly instrument, full of capabilities.
2024 Publications
Boyanova, S.T. et al. (2023) ‘Interaction of amisulpride with GLUT1 at the blood-brain barrier. Relevance to Alzheimer’s disease’, PLOS ONE, 18(10), p. e0286278. Available at: https://doi.org/10.1371/journal.pone.0286278.
Burkitt-Gray, M. et al. (2023) ‘Structural investigations into colour-tuneable fluorescent InZnP-based quantum dots from zinc carboxylate and aminophosphine precursors’, Nanoscale, 15(4), pp. 1763–1774. Available at: https://doi.org/10.1039/D2NR02803D.
Horton, S. et al. (2024) ‘Excitatory and inhibitory synapses show a tight subcellular correlation that weakens over development’, Cell Reports, 43(7). Available at: https://doi.org/10.1016/j.celrep.2024.114361.
Kenny, F.N. et al. (2023) ‘Autocrine IL-6 drives cell and extracellular matrix anisotropy in scar fibroblasts’, Matrix Biology, 123, pp. 1–16. Available at: https://doi.org/10.1016/j.matbio.2023.08.004.
Nicholls, D. et al. (2024) ‘The Potential of Subsampling and Inpainting for Fast Low-Dose Cryo FIB-SEM Imaging’, Microscopy and Microanalysis, 30(1), pp. 96–102. Available at: https://doi.org/10.1093/micmic/ozae005.
Velazco, A. et al. (2024) ‘Reduction of SEM charging artefacts in native cryogenic biological samples’. bioRxiv, p. 2024.08.23.609373. Available at: https://doi.org/10.1101/2024.08.23.609373.
Wilson, S. et al. (2024) ‘Hydrophobic Mismatch in the Thylakoid Membrane Regulates Photosynthetic Light Harvesting’, Journal of the American Chemical Society, 146(21), pp. 14905–14914. Available at: https://doi.org/10.1021/jacs.4c05220.
2023 Publications
Boyanova, S.T. et al. (2023) ‘Interaction of amisulpride with GLUT1 at the blood-brain barrier. Relevance to Alzheimer’s disease’, PLOS ONE, 18(10), p. e0286278. Available at: https://doi.org/10.1371/journal.pone.0286278.
Burkitt-Gray, M. et al. (2023) ‘Structural investigations into colour-tuneable fluorescent InZnP-based quantum dots from zinc carboxylate and aminophosphine precursors’, Nanoscale, 15(4), pp. 1763–1774. Available at: https://doi.org/10.1039/D2NR02803D.
Kenny, F.N. et al. (2023) ‘Autocrine IL-6 drives cell and extracellular matrix anisotropy in scar fibroblasts’, Matrix Biology, 123, pp. 1–16. Available at: https://doi.org/10.1016/j.matbio.2023.08.004.
Morfill, C. et al. (2023) ‘Addition to “Nanostars Carrying Multifunctional Neurotrophic Dendrimers Protect Neurons in Preclinical In Vitro Models of Neurodegenerative Disorders”’, ACS Applied Materials & Interfaces, 15(10), pp. 13824–13824. Available at: https://doi.org/10.1021/acsami.3c02300.
Naso, G. et al. (2023) ‘Cytosine Deaminase Base Editing to Restore COL7A1 in Dystrophic Epidermolysis Bullosa Human: Murine Skin Model’, JID Innovations, 3(3), p. 100191. Available at: https://doi.org/10.1016/j.xjidi.2023.100191.
Rigby, M. et al. (2023) ‘Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens’, Cell Reports, 42(5), p. 112397. Available at: https://doi.org/10.1016/j.celrep.2023.112397.
Zhang, W. et al. (2023) ‘Characterising the chemical and physical properties of phase-change nanodroplets’, Ultrasonics Sonochemistry, 97, p. 106445. Available at: https://doi.org/10.1016/j.ultsonch.2023.106445.
2022 Publications
Mórotz, G.M. et al. (2022) ‘The PTPIP51 coiled-coil domain is important in VAPB binding, formation of ER-mitochondria contacts and IP3 receptor delivery of Ca2+ to mitochondria’, Frontiers in Cell and Developmental Biology, 10. Available at: https://doi.org/10.3389/fcell.2022.920947.
2021 Publications
Wallis, S., Ventimiglia, L., Otigbah, E., Infante, E., Cuesta-Geijo, M., Kidiyoor, G., Carbajal, M., Fleck, R., Foiani, M., Garcia-Manyes, S., Martin-Serrano, J. and Agromayor, M., 2021. The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair. Developmental Cell, 56(23), pp.3192-3202.e8.
2020 Publications
Matsubayashi, Y., Sánchez-Sánchez, B., Marcotti, S., Serna-Morales, E., Dragu, A., Díaz-de-la-Loza, M., Vizcay-Barrena, G., Fleck, R. and Stramer, B., 2020. Rapid Homeostatic Turnover of Embryonic ECM during Tissue Morphogenesis. Developmental Cell, 54(1), pp.33-42.e9.
Valente, P., Kiryushko, D., Sacchetti, S., Machado, P., Cobley, C., Mangini, V., Porter, A., Spatz, J., Fleck, R., Benfenati, F. and Fiammengo, R., 2020. Conopeptide-Functionalized Nanoparticles Selectively Antagonize ExtrasynapticN-Methyl-d-aspartate Receptors and Protect Hippocampal Neurons from Excitotoxicity In Vitro. ACS Nano, 14(6), pp.6866-6877.
Awan, M., Buriak, I., Fleck, R., Fuller, B., Goltsev, A., Kerby, J., Lowdell, M., Mericka, P., Petrenko, A., Petrenko, Y., Rogulska, O., Stolzing, A. and Stacey, G., 2020 Dimethyl sulfoxide: a central player since the dawn of cryobiology, is efficacy balanced by toxicity? Regenerative Medicine, 15(3), pp.1463-1491.
Perrotta, S., Roberti, D., Bencivenga, D., Corsetto, P., O’Brien, K., Caiazza, M., Stampone, E., Allison, L., Fleck, R., Scianguetta, S., Tartaglione, I., Robbins, P., Casale, M., West, J., Franzini-Armstrong, C., Griffin, J., Rizzo, A., Sinisi, A., Murray, A., Borriello, A., Formenti, F. and Della Ragione, F., 2020. Effects of Germline VHL Deficiency on Growth, Metabolism, and Mitochondria. New England Journal of Medicine, 382(9), pp.835-844.
Hau, H., Ogundele, O., Hibbert, A., Monfries, C., Exelby, K., Wood, N., Nevarez-Mejia, J., Carbajal, M., Fleck, R., Dermit, M., Mardakheh, F., Williams-Ward, V., Pipalia, T., Conte, M. and Hughes, S., 2020. Maternal Larp6 controls oocyte development, chorion formation and elevation. Development
Petrova, A., Georgiadis, C., Fleck, R., Allison, L., McGrath, J., Dazzi, F., Di, W. and Qasim, W., 2020. Human Mesenchymal Stromal Cells Engineered to Express Collagen VII Can Restore Anchoring Fibrils in Recessive Dystrophic Epidermolysis Bullosa Skin Graft Chimeras. Journal of Investigative Dermatology, 140(1), pp.121-131.e6.
2019 Publications
Veraitch, O., Allison, L., Vizcay‐Barrena, G., Fleck, R., Price, A., Fenton, D., McGrath, J. and Stefanato, C., 2019. Detailed hair shaft analysis in a man with delayed‐onset Chediak‐Higashi syndrome. British Journal of Dermatology.
2017 Publications
So, E., Mitchell, J., Memmi, C., Chennell, G., Vizcay-Barrena, G., Allison, L., Shaw, C. and Vance, C., 2017. Mitochondrial abnormalities and disruption of the neuromuscular junction precede the clinical phenotype and motor neuron loss in hFUSWT transgenic mice. Human Molecular Genetics, 27(3), pp.463-474.
Jitraruch, S., Dhawan, A., Hughes, R., Filippi, C., Lehec, S., Glover, L. and Mitry, R., 2017. Cryopreservation of Hepatocyte Microbeads for Clinical Transplantation. Cell Transplantation, 26(8), pp.1341-1354.
Hale, V. L., Watermeyer, J. M., Hackett, F., Vizcay-Barrena, G., Van Ooij, C., Thomas, J. A., Spink, M. C., Harkiolaki, M., Duke, E., Fleck, R. A., Blackman, M. J. & Saibil, H. R. 28 Mar 2017 Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress In : Proceedings of the National Academy of Sciences of the United States of America. 114, 13, p. 3439-3444.
Walker, A. S., Neves, G., Grillo, F., Jackson, R. E., Rigby, M., O’Donnell, C., Lowe, A. S., Vizcay-Barrena, G., Fleck, R. A. & Burrone, J. 7 Mar 2017 Distance-dependent gradient in NMDAR-driven spine calcium signals along tapering dendritesIn : Proceedings of the National Academy of Sciences of the United States of America. 114, 10, p. E1986-E1995.
Sekhar, G. N., Georgian, A. R., Sanderson, L., Vizcay-Barrena, G., Brown, R. C., Muresan, P., Fleck, R. A. & Thomas, S. A.1 Mar 2017 Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB) In : PL o S One . 12, 3, e0173474.
2016 Publications
Liakath-Ali, K., Vancollie, V., Lelliott, C., Speak, A., Lafont, D., Protheroe, H., Ingvorsen, C., Galli, A., Green, A., Gleeson, D., Ryder, E., Glover, L., Vizcay-Barrena, G., Karp, N., Arends, M., Brenn, T., Spiegel, S., Adams, D., Watt, F. and van der Weyden, L., 2016. Alkaline ceramidase 1 is essential for mammalian skin homeostasis and regulating whole-body energy expenditure. The Journal of Pathology, 239(3), pp.374-383.
Lussignol, M., Kopp, M., Molloy, K., Vizcay-Barrena, G., Fleck, R. A., Dorner, M., Bell, K. L., Chait, B. T., Rice, C. M. & Catanese, M. T. 1 Mar 2016. Proteomics of HCV virions reveals an essential role for the nucleoporin Nup98 in virus morphogenesis In : Proceedings of the National Academy of Sciences of the United States of America. 113, 9, p. 2484-2489.
Chapple, S. J., Keeley, T. P., Mastronicola, D., Arno, M., Vizcay-Barrena, G., Fleck, R., Siow, R. & Mann, G. Mar 2016. Bach1 differentially regulates distinct Nrf2-dependent genes in human venous and coronary artery endothelial cells adapted to physiological oxygen levels In : Free radical biology & medicine. 92, p. 152-162.
Veraitch, O., Perez, A., Hoque, S. R., Vizcay-Barrena, G., Fleck, R. A., Fenton, D. A. & Stefanato, C. M. Mar 2016. Hair Follicle Miniaturization in a Woolly Hair Nevus: A Novel “Root” Perspective for a Mosaic Hair Disorder In : American Journal of Dermatopathology. 38, 3, p. 239-243.
Wbp2 is required for normal glutamatergic synapses in the cochlea and is crucial for hearingBuniello, A., Ingham, N. J., Lewis, M. A., Huma, A. C., Martinez-Vega, R., Varela-Nieto, I., Vizcay-Barrena, G., Fleck, R. A., Houston, O., Bardhan, T., Johnson, S. L., White, J. K., Yuan, H., Marcotti, W. & Steel, K. P. 8 Feb 2016 In : EMBO Molecular Medicine. 8, 3, p. 191.-207.
Green, M., Haigh, S. J., Lewis, E. A., Sandiford, L., Burkitt-Gray, M., Fleck, R., Vizcay-Barrena, G., Jensen, L., Mirzai, H., Curry, R. J. & Dailey, L. A. 9 Feb 2016 The Biosynthesis of Infrared-Emitting Quantum Dots in Allium Fistulosum In : Scientific Reports. 6, 20480.
Georgiadis, C., Syed, F., Petrova, A., Abdul-Wahab, A., Lwin, S., Farzaneh, F., Chan, L., Ghani, S., Fleck, R., Glover, L., McMillan, J., Chen, M., Thrasher, A., McGrath, J., Di, W. and Qasim, W., 2016. Lentiviral Engineered Fibroblasts Expressing Codon-Optimized COL7A1 Restore Anchoring Fibrils in RDEB. Journal of Investigative Dermatology, 136(1), pp.284-292.
Watermeyer, J. M., Hale, V. L., Hackett, F., Clare, D. K., Cutts, E. E., Vakonakis, I., Fleck, R., Blackman, M. J. & Saibil, H. R. 21 Jan 2016. A spiral scaffold underlies cytoadherent knobs in Plasmodium falciparum-infected erythrocytes In : Blood. 127, 3, p. 343-351.
2015 Publications
Mitchell, J., Constable, R., So, E., Vance, C., Scotter, E., Glover, L., Hortobagyi, T., Arnold, E., Ling, S., McAlonis, M., Da Cruz, S., Polymenidou, M., Tessarolo, L., Cleveland, D. and Shaw, C., 2015. Wild type human TDP-43 potentiates ALS-linked mutant TDP-43 driven progressive motor and cortical neuron degeneration with pathological features of ALS. Acta Neuropathologica Communications, 3(1).
Fleck, R.A. (2015) Low temperature electron microscopy, in, Methods in Cryopreservation and Freeze-Drying (edsW.Wolkers and H. Oldenhof), Lab Protocol Series, Methods in Molecular Biology, Springer, Berlin, pp. 243–274.
2013 Publications
Carlin LM, Stamatiades EG, Auffray C, Hanna RN, Glover L, Vizcay-Barrena G, Hedrick CC, Cook HT, Diebold S, Geissmann F. Cell. 2013 Apr. Nr4a1-dependent Ly6C(low) monocytes monitor endothelial cells and orchestrate their disposal. 11;153(2):362-75.
We are dedicated to promoting collaborative research and providing wider access to our state-of-the-art core facilities. The Centre for Ultrastructural Imaging is open to King’s researchers and external academic and industry users.
Anyone interested on accessing the facility should contact the centre at cui@kcl.ac.uk for an initial project discussion. Our team will provide advice on experimental design, training, and the most appropriate technical approach for your project
Booking equipment
Trained users can book equipment via our via the booking system.
Publication and acknowledgement
Where a significant contribution has been made, researchers are expected to acknowledge the contribution of CUI staff by way of authorship, in accordance with the Core Facilities Fair Publication Policy.
We provide cutting-edge and comprehensive workflows to support life and materials science electron microscopy.
We work with leading international research groups, as well as instrument manufacturers to support advanced electron microscopy techniques and promote novel ideas and innovation.
Techniques available in the CUI.
- Transmission Electron Microscopy (TEM)High-resolution images of nanoscale structures within cells and tissues.
- Scanning Electron Microscopy (SEM)Using SEM, we are able to generate an image showing topographical detail.
- Critical Point DryingCritical point drying preserves ultrastructure and provide a room temperature stable sample for imaging in a scanning electron microscope.
- High-pressure freezing and freeze substitutionHPF is a cryo-fixation method that allows the study of biological specimens with improved preservation of cellular structures.
- Energy Dispersive X-ray Spectroscopy (EDS)Energy Dispersive x-ray Spectroscopy is used to determine and quantify the elemental composition of a sample.
- Electron Tomography (ET)ET is a powerful technique to generate a detailed 3D volume which reveals structures of sub-cellular macro-molecular objects at the nanometre scale.
- Scanning Transmission Electron Microscopy (STEM)A scanning transmission electron microscope is equipped with additional scanning coils, detectors and necessary circuitry.
- Serial Block Face Scanning Electron Microscopy (SBF-SEM)SBF-SEM an automated 3D imaging technique that combines in situ ultramicrotomy with scanning electron microscopy.
- Correlative Light and Electron Microscopy (CLEM)CLEM is an imaging technique that combines fluorescence light microscopy (FM) and electron microscopy (EM) to analyse the same biological sample.
- Plunge FreezingPlunge freezing is a cryo-fixation technique used to fix delicate samples for cryo-Electron Microscopy.
Facility staff
Facility Manager - Centre for Ultrastructural Imaging
Electron Microscopy Applications Specialist
Electron Microscopy Technician
Affiliated academics
Associate Director Innovation & Business Development – Imaging Core Facilities and Smart Trials Hub
Business Systems and Engagement Coordinator
Equipment available at the CUI
Related equipment
JEOL JEM-1400Flash Transmission Electron Microscope (TEM)
The JEOL JEM-1400Flash is used to study a variety of materials from nanoparticles, viruses, bacteria, and sections of cells and tissues.
JEOL JEM-F200 Transmission Electron Microscope (TEM)
The JEOL JEM-F200 is our 200Kv Transmission Electron Microscope for advanced room temperature applications such as electron tomography.
JEOL JSM 7800F Prime Scanning Electron Microscope (SEM)
The JEOL JSM 7800F Prime SEM is our current top end SEM, equipped with a Schottky Field Emission Gun.
JEOL JSM 7800F Serial Block Face-Scanning Electron Microscope (SBF-SEM)
The JSM 7800F Serial Block Face SEM is equipped with a ConnectomX Katana microtome for volume EM.
JEOL JCM 7000 NeoScope Scanning Electron Microscope (SEM)
The JEOL JCM 7000 NeoScope is our benchtop scanning electron microscope (SEM). A small size, user friendly instrument, full of capabilities.
2024 Publications
Boyanova, S.T. et al. (2023) ‘Interaction of amisulpride with GLUT1 at the blood-brain barrier. Relevance to Alzheimer’s disease’, PLOS ONE, 18(10), p. e0286278. Available at: https://doi.org/10.1371/journal.pone.0286278.
Burkitt-Gray, M. et al. (2023) ‘Structural investigations into colour-tuneable fluorescent InZnP-based quantum dots from zinc carboxylate and aminophosphine precursors’, Nanoscale, 15(4), pp. 1763–1774. Available at: https://doi.org/10.1039/D2NR02803D.
Horton, S. et al. (2024) ‘Excitatory and inhibitory synapses show a tight subcellular correlation that weakens over development’, Cell Reports, 43(7). Available at: https://doi.org/10.1016/j.celrep.2024.114361.
Kenny, F.N. et al. (2023) ‘Autocrine IL-6 drives cell and extracellular matrix anisotropy in scar fibroblasts’, Matrix Biology, 123, pp. 1–16. Available at: https://doi.org/10.1016/j.matbio.2023.08.004.
Nicholls, D. et al. (2024) ‘The Potential of Subsampling and Inpainting for Fast Low-Dose Cryo FIB-SEM Imaging’, Microscopy and Microanalysis, 30(1), pp. 96–102. Available at: https://doi.org/10.1093/micmic/ozae005.
Velazco, A. et al. (2024) ‘Reduction of SEM charging artefacts in native cryogenic biological samples’. bioRxiv, p. 2024.08.23.609373. Available at: https://doi.org/10.1101/2024.08.23.609373.
Wilson, S. et al. (2024) ‘Hydrophobic Mismatch in the Thylakoid Membrane Regulates Photosynthetic Light Harvesting’, Journal of the American Chemical Society, 146(21), pp. 14905–14914. Available at: https://doi.org/10.1021/jacs.4c05220.
2023 Publications
Boyanova, S.T. et al. (2023) ‘Interaction of amisulpride with GLUT1 at the blood-brain barrier. Relevance to Alzheimer’s disease’, PLOS ONE, 18(10), p. e0286278. Available at: https://doi.org/10.1371/journal.pone.0286278.
Burkitt-Gray, M. et al. (2023) ‘Structural investigations into colour-tuneable fluorescent InZnP-based quantum dots from zinc carboxylate and aminophosphine precursors’, Nanoscale, 15(4), pp. 1763–1774. Available at: https://doi.org/10.1039/D2NR02803D.
Kenny, F.N. et al. (2023) ‘Autocrine IL-6 drives cell and extracellular matrix anisotropy in scar fibroblasts’, Matrix Biology, 123, pp. 1–16. Available at: https://doi.org/10.1016/j.matbio.2023.08.004.
Morfill, C. et al. (2023) ‘Addition to “Nanostars Carrying Multifunctional Neurotrophic Dendrimers Protect Neurons in Preclinical In Vitro Models of Neurodegenerative Disorders”’, ACS Applied Materials & Interfaces, 15(10), pp. 13824–13824. Available at: https://doi.org/10.1021/acsami.3c02300.
Naso, G. et al. (2023) ‘Cytosine Deaminase Base Editing to Restore COL7A1 in Dystrophic Epidermolysis Bullosa Human: Murine Skin Model’, JID Innovations, 3(3), p. 100191. Available at: https://doi.org/10.1016/j.xjidi.2023.100191.
Rigby, M. et al. (2023) ‘Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens’, Cell Reports, 42(5), p. 112397. Available at: https://doi.org/10.1016/j.celrep.2023.112397.
Zhang, W. et al. (2023) ‘Characterising the chemical and physical properties of phase-change nanodroplets’, Ultrasonics Sonochemistry, 97, p. 106445. Available at: https://doi.org/10.1016/j.ultsonch.2023.106445.
2022 Publications
Mórotz, G.M. et al. (2022) ‘The PTPIP51 coiled-coil domain is important in VAPB binding, formation of ER-mitochondria contacts and IP3 receptor delivery of Ca2+ to mitochondria’, Frontiers in Cell and Developmental Biology, 10. Available at: https://doi.org/10.3389/fcell.2022.920947.
2021 Publications
Wallis, S., Ventimiglia, L., Otigbah, E., Infante, E., Cuesta-Geijo, M., Kidiyoor, G., Carbajal, M., Fleck, R., Foiani, M., Garcia-Manyes, S., Martin-Serrano, J. and Agromayor, M., 2021. The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair. Developmental Cell, 56(23), pp.3192-3202.e8.
2020 Publications
Matsubayashi, Y., Sánchez-Sánchez, B., Marcotti, S., Serna-Morales, E., Dragu, A., Díaz-de-la-Loza, M., Vizcay-Barrena, G., Fleck, R. and Stramer, B., 2020. Rapid Homeostatic Turnover of Embryonic ECM during Tissue Morphogenesis. Developmental Cell, 54(1), pp.33-42.e9.
Valente, P., Kiryushko, D., Sacchetti, S., Machado, P., Cobley, C., Mangini, V., Porter, A., Spatz, J., Fleck, R., Benfenati, F. and Fiammengo, R., 2020. Conopeptide-Functionalized Nanoparticles Selectively Antagonize ExtrasynapticN-Methyl-d-aspartate Receptors and Protect Hippocampal Neurons from Excitotoxicity In Vitro. ACS Nano, 14(6), pp.6866-6877.
Awan, M., Buriak, I., Fleck, R., Fuller, B., Goltsev, A., Kerby, J., Lowdell, M., Mericka, P., Petrenko, A., Petrenko, Y., Rogulska, O., Stolzing, A. and Stacey, G., 2020 Dimethyl sulfoxide: a central player since the dawn of cryobiology, is efficacy balanced by toxicity? Regenerative Medicine, 15(3), pp.1463-1491.
Perrotta, S., Roberti, D., Bencivenga, D., Corsetto, P., O’Brien, K., Caiazza, M., Stampone, E., Allison, L., Fleck, R., Scianguetta, S., Tartaglione, I., Robbins, P., Casale, M., West, J., Franzini-Armstrong, C., Griffin, J., Rizzo, A., Sinisi, A., Murray, A., Borriello, A., Formenti, F. and Della Ragione, F., 2020. Effects of Germline VHL Deficiency on Growth, Metabolism, and Mitochondria. New England Journal of Medicine, 382(9), pp.835-844.
Hau, H., Ogundele, O., Hibbert, A., Monfries, C., Exelby, K., Wood, N., Nevarez-Mejia, J., Carbajal, M., Fleck, R., Dermit, M., Mardakheh, F., Williams-Ward, V., Pipalia, T., Conte, M. and Hughes, S., 2020. Maternal Larp6 controls oocyte development, chorion formation and elevation. Development
Petrova, A., Georgiadis, C., Fleck, R., Allison, L., McGrath, J., Dazzi, F., Di, W. and Qasim, W., 2020. Human Mesenchymal Stromal Cells Engineered to Express Collagen VII Can Restore Anchoring Fibrils in Recessive Dystrophic Epidermolysis Bullosa Skin Graft Chimeras. Journal of Investigative Dermatology, 140(1), pp.121-131.e6.
2019 Publications
Veraitch, O., Allison, L., Vizcay‐Barrena, G., Fleck, R., Price, A., Fenton, D., McGrath, J. and Stefanato, C., 2019. Detailed hair shaft analysis in a man with delayed‐onset Chediak‐Higashi syndrome. British Journal of Dermatology.
2017 Publications
So, E., Mitchell, J., Memmi, C., Chennell, G., Vizcay-Barrena, G., Allison, L., Shaw, C. and Vance, C., 2017. Mitochondrial abnormalities and disruption of the neuromuscular junction precede the clinical phenotype and motor neuron loss in hFUSWT transgenic mice. Human Molecular Genetics, 27(3), pp.463-474.
Jitraruch, S., Dhawan, A., Hughes, R., Filippi, C., Lehec, S., Glover, L. and Mitry, R., 2017. Cryopreservation of Hepatocyte Microbeads for Clinical Transplantation. Cell Transplantation, 26(8), pp.1341-1354.
Hale, V. L., Watermeyer, J. M., Hackett, F., Vizcay-Barrena, G., Van Ooij, C., Thomas, J. A., Spink, M. C., Harkiolaki, M., Duke, E., Fleck, R. A., Blackman, M. J. & Saibil, H. R. 28 Mar 2017 Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress In : Proceedings of the National Academy of Sciences of the United States of America. 114, 13, p. 3439-3444.
Walker, A. S., Neves, G., Grillo, F., Jackson, R. E., Rigby, M., O’Donnell, C., Lowe, A. S., Vizcay-Barrena, G., Fleck, R. A. & Burrone, J. 7 Mar 2017 Distance-dependent gradient in NMDAR-driven spine calcium signals along tapering dendritesIn : Proceedings of the National Academy of Sciences of the United States of America. 114, 10, p. E1986-E1995.
Sekhar, G. N., Georgian, A. R., Sanderson, L., Vizcay-Barrena, G., Brown, R. C., Muresan, P., Fleck, R. A. & Thomas, S. A.1 Mar 2017 Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB) In : PL o S One . 12, 3, e0173474.
2016 Publications
Liakath-Ali, K., Vancollie, V., Lelliott, C., Speak, A., Lafont, D., Protheroe, H., Ingvorsen, C., Galli, A., Green, A., Gleeson, D., Ryder, E., Glover, L., Vizcay-Barrena, G., Karp, N., Arends, M., Brenn, T., Spiegel, S., Adams, D., Watt, F. and van der Weyden, L., 2016. Alkaline ceramidase 1 is essential for mammalian skin homeostasis and regulating whole-body energy expenditure. The Journal of Pathology, 239(3), pp.374-383.
Lussignol, M., Kopp, M., Molloy, K., Vizcay-Barrena, G., Fleck, R. A., Dorner, M., Bell, K. L., Chait, B. T., Rice, C. M. & Catanese, M. T. 1 Mar 2016. Proteomics of HCV virions reveals an essential role for the nucleoporin Nup98 in virus morphogenesis In : Proceedings of the National Academy of Sciences of the United States of America. 113, 9, p. 2484-2489.
Chapple, S. J., Keeley, T. P., Mastronicola, D., Arno, M., Vizcay-Barrena, G., Fleck, R., Siow, R. & Mann, G. Mar 2016. Bach1 differentially regulates distinct Nrf2-dependent genes in human venous and coronary artery endothelial cells adapted to physiological oxygen levels In : Free radical biology & medicine. 92, p. 152-162.
Veraitch, O., Perez, A., Hoque, S. R., Vizcay-Barrena, G., Fleck, R. A., Fenton, D. A. & Stefanato, C. M. Mar 2016. Hair Follicle Miniaturization in a Woolly Hair Nevus: A Novel “Root” Perspective for a Mosaic Hair Disorder In : American Journal of Dermatopathology. 38, 3, p. 239-243.
Wbp2 is required for normal glutamatergic synapses in the cochlea and is crucial for hearingBuniello, A., Ingham, N. J., Lewis, M. A., Huma, A. C., Martinez-Vega, R., Varela-Nieto, I., Vizcay-Barrena, G., Fleck, R. A., Houston, O., Bardhan, T., Johnson, S. L., White, J. K., Yuan, H., Marcotti, W. & Steel, K. P. 8 Feb 2016 In : EMBO Molecular Medicine. 8, 3, p. 191.-207.
Green, M., Haigh, S. J., Lewis, E. A., Sandiford, L., Burkitt-Gray, M., Fleck, R., Vizcay-Barrena, G., Jensen, L., Mirzai, H., Curry, R. J. & Dailey, L. A. 9 Feb 2016 The Biosynthesis of Infrared-Emitting Quantum Dots in Allium Fistulosum In : Scientific Reports. 6, 20480.
Georgiadis, C., Syed, F., Petrova, A., Abdul-Wahab, A., Lwin, S., Farzaneh, F., Chan, L., Ghani, S., Fleck, R., Glover, L., McMillan, J., Chen, M., Thrasher, A., McGrath, J., Di, W. and Qasim, W., 2016. Lentiviral Engineered Fibroblasts Expressing Codon-Optimized COL7A1 Restore Anchoring Fibrils in RDEB. Journal of Investigative Dermatology, 136(1), pp.284-292.
Watermeyer, J. M., Hale, V. L., Hackett, F., Clare, D. K., Cutts, E. E., Vakonakis, I., Fleck, R., Blackman, M. J. & Saibil, H. R. 21 Jan 2016. A spiral scaffold underlies cytoadherent knobs in Plasmodium falciparum-infected erythrocytes In : Blood. 127, 3, p. 343-351.
2015 Publications
Mitchell, J., Constable, R., So, E., Vance, C., Scotter, E., Glover, L., Hortobagyi, T., Arnold, E., Ling, S., McAlonis, M., Da Cruz, S., Polymenidou, M., Tessarolo, L., Cleveland, D. and Shaw, C., 2015. Wild type human TDP-43 potentiates ALS-linked mutant TDP-43 driven progressive motor and cortical neuron degeneration with pathological features of ALS. Acta Neuropathologica Communications, 3(1).
Fleck, R.A. (2015) Low temperature electron microscopy, in, Methods in Cryopreservation and Freeze-Drying (edsW.Wolkers and H. Oldenhof), Lab Protocol Series, Methods in Molecular Biology, Springer, Berlin, pp. 243–274.
2013 Publications
Carlin LM, Stamatiades EG, Auffray C, Hanna RN, Glover L, Vizcay-Barrena G, Hedrick CC, Cook HT, Diebold S, Geissmann F. Cell. 2013 Apr. Nr4a1-dependent Ly6C(low) monocytes monitor endothelial cells and orchestrate their disposal. 11;153(2):362-75.
We are dedicated to promoting collaborative research and providing wider access to our state-of-the-art core facilities. The Centre for Ultrastructural Imaging is open to King’s researchers and external academic and industry users.
Anyone interested on accessing the facility should contact the centre at cui@kcl.ac.uk for an initial project discussion. Our team will provide advice on experimental design, training, and the most appropriate technical approach for your project
Booking equipment
Trained users can book equipment via our via the booking system.
Publication and acknowledgement
Where a significant contribution has been made, researchers are expected to acknowledge the contribution of CUI staff by way of authorship, in accordance with the Core Facilities Fair Publication Policy.
