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The Centre for Ultrastructural Imaging (CUI) is the central electron microscopy facility at Kings College London. We offer a state-of-the-art facility for internal and external collaborators from academic, commercial, and industrial fields across medicine, biological sciences, chemistry, physics, materials sciences, and engineering.

Our vision is to provide expert electron microscopy services and support to research groups as well as training new electron microscopists in advanced techniques.

Collaboration

We operate as a collaborative research facility and support researchers with experiment design and data interpretation ensuring that high-quality data is collected and prepared for publication. Our staff can assist with method development, materials, figure presentation, appropriate references, training on specialised equipment and data interpretation.

Where a significant contribution has been identified, researchers are expected to acknowledge the contribution of CUI staff by way of authorship, in accordance with the Core Facilities Fair Publication Policy.

We provide cutting-edge and comprehensive workflows to support life and materials science electron microscopy.  Electron microscopes come in a variety of shapes and sizes and when used correctly, they are powerful investigative tools, capable of revealing a fascinating miniature world which is not visible with the naked eye, or even with a light microscope.

We work with leading international research groups, as well as instrument manufacturers to support advanced electron microscopy techniques and promote novel ideas and innovation.  

Here are some of the techniques available in the CUI.

Facility staff

Roland

Director of Centre for Ultrastructural Imaging

Leanne

Facility Manager - Centre for Ultrastructural Imaging

James Gereats

Cryo-Electron Microscopy Application Specialist

Matt

Electron Microscopy Applications Specialist

Ale

Electron Microscopy Technician

Shannon Stockdale2

Electron Microscopy Technician

Equipment available at the CUI

Related equipment

JEOL 1400Plus FLASH
JEOL JEM-1400Flash

The JEOL JEM-1400Flash is used to study a variety of materials from nanoparticles, viruses, bacteria, and sections of cells and tissues.

F200 picture
JEOL JEM-F200 TEM

The JEOL JEM-F200 is our current cryo-TEM and is used for screening of cryo-samples as well as room temperature applications such as tomography.

7800 Prime b
JEOL JSM 7800F Prime Cryo-SEM

The JEOL JSM 7800F Prime SEM is our current top end SEM, equipped with a Schottky FEG and has the capability to run in cryo mode.

JSM-7800 a
JEOL JSM 7800F SBF-SEM

The JSM 7800F Serial Block Face SEM is the same microscope as our top end JSM 7800F Prime Cryo-SEM and it is equipped with a katana microtome for volume EM.

4. Scanning Transmission Electron Microscopy- High Angle Annular Darkfield Imaging (STEM-HAADF)
JEOL JIB-4700F Z Dual Beam FIB-SEM

The microscope is used to create lamellae for electron tomography and volume electron microscopy

JEOL JCM7000 NEOSCOPE
JEOL JCM 7000 NeoScope Benchtop SEM

The JEOL JCM 7000 NeoScope is our smallest electron microscope, but despite its small size it is a very capable instrument packed with features.

2023

Burkitt Grey, M., Fleck, RA., Casavola, M., Clark, P., Fairclough, S., Flavell, W., Haigh, S., Chun-Ren Ke, J., Leontiadou, M., Lewis, E., Osiecki, J., Qazi-Chaudhry, B., Vizcay-Barrena, G., Wichiansee, W., Green., M. (2023), Structural investigations into colour-tuneable fluorescent InZnP-based quantum dots from zinc carboxylate and aminophosphine precursors, Nanoscale.2023.10.1039.

Petrova, A., Allison, L., Fleck, RA., Naso, G., Athina Gkazi, S., Georgiadis, C., Jayarajan, V., Jacków, J., Kayode Ogunbiyi, O., McGrath, JA., Ilic, D., Di, W., Qasim, W. (2023), Cytosine Deaminase Base Editing to Restore COL7A1in Dystrophic Epidermolysis Bullosa Human: Murine Skin Model, JID Innovations.2023.10.1016

Morfill, C., Fleck, RA., Pankratova, S., Machado, P., Fernado, NK., Regoutz, A., Talamona, F., Pinna, A., Klosowski, M., Wilkinson, R., Xie, Fang., Porter, A., Kiryushko, D. (2023), Addition to "Nanostars Carrying Multifunctional Neurotrophic Dendrimers Protect Neurons in Preclinical In Vitro Models of Neurodegenerative Disorders", ACS Applied Materials & Interfaces.2023.10.1021.

Rigby, M., Fleck, RA., Grillo, F., Compans, B., Neves, G., Gallinaro, J., Nashashibi, S., Horton, S., Pereira Machado, P., Carbajal, MA., Vizcay-Barrena, G., Levet, F., Sibarita, JB., Kirkland, A., Clopath, C., Burrone, J. (2023), Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens, Cell Reports.2023.10.1016.

Bille, A., Allison, L., Fleck, RA., Russell, M., Jhala, H., Ashrafian, L., Nonaka, D. (2023), Does hyperthermic povidone-iodine lavage increase the apoptotic rate of residual cancer cell in patients with malignant pleural mesothelioma? -A prospective pilot study, Translational Lung Cancer Research.2023.10.21037.

Kobylynska, M., Fleck, RA. Nicholls, D., Wells, J., Robinson, A., Moshtaghpour, A., Kobylynska, M., Kirkland, A., Mehdi, L., Browning, N. (2023), Compressive Cryo FIB-SEM Tomography, Microscopy and Microanalysis. Microscopy and Microanalysis.2023.0.1093

Zhang, W., Fleck, RA., Carbajal, AM., Metzger, H., Vlatakis, S., Claxton, A., Fung, LF., Mason, J., Chan, KL.A., Pouliopoulos, A., Prentice, P., Thanou, M. (2023), Characterising the chemical and physical properties of phase-change nanodroplets, Ultrasonics Sonochemistry.2023.10.10.16.

2022

Morotz M., Fleck, RA., Russell, M., Martín-Guerrero, S., Markovinovic, A., Paillusson, S., Pereira Machado, P., Noble, W., Miller, C. (2022), The PTPIP51 coiled-coil domain is important in VAPB binding, formation of ER-mitochondria contacts and IP3 receptor delivery of Ca2+ to mitochondria, Frontiers in cell and developmental biology, Frontiers in Cell.2022.10.3389.

Bernard, C., Allison, L., Fleck, RA., Selten, J., Hanusz-Godoy, A., Aguilera, A., Hamid, F., Maeso, P., Russell, M., Oozeer, F., Sanalidou, S., Exposito-Alonso, D., Rico, B., Marín, O. (2022), Cortical wiring by synapse type–specific control of local protein synthesis, Science.2022.0.1126. 

2021

Wallis, S., Ventimiglia, L., Otigbah, E., Infante, E., Cuesta-Geijo, M., Kidiyoor, G., Carbajal, M., Fleck, R., Foiani, M., Garcia-Manyes, S., Martin-Serrano, J. and Agromayor, M., 2021. The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.  Developmental Cell, 56(23), pp.3192-3202.e8.

2020

Matsubayashi, Y., Sánchez-Sánchez, B., Marcotti, S., Serna-Morales, E., Dragu, A., Díaz-de-la-Loza, M., Vizcay-Barrena, G., Fleck, R. and Stramer, B., 2020. Rapid Homeostatic Turnover of Embryonic ECM during Tissue Morphogenesis. Developmental Cell, 54(1), pp.33-42.e9.

Valente, P., Kiryushko, D., Sacchetti, S., Machado, P., Cobley, C., Mangini, V., Porter, A., Spatz, J., Fleck, R., Benfenati, F. and Fiammengo, R., 2020. Conopeptide-Functionalized Nanoparticles Selectively Antagonize ExtrasynapticN-Methyl-d-aspartate Receptors and Protect Hippocampal Neurons from Excitotoxicity In Vitro. ACS Nano, 14(6), pp.6866-6877.

Awan, M., Buriak, I., Fleck, R., Fuller, B., Goltsev, A., Kerby, J., Lowdell, M., Mericka, P., Petrenko, A., Petrenko, Y., Rogulska, O., Stolzing, A. and Stacey, G., 2020 Dimethyl sulfoxide: a central player since the dawn of cryobiology, is efficacy balanced by toxicity? Regenerative Medicine, 15(3), pp.1463-1491.

Perrotta, S., Roberti, D., Bencivenga, D., Corsetto, P., O’Brien, K., Caiazza, M., Stampone, E., Allison, L., Fleck, R., Scianguetta, S., Tartaglione, I., Robbins, P., Casale, M., West, J., Franzini-Armstrong, C., Griffin, J., Rizzo, A., Sinisi, A., Murray, A., Borriello, A., Formenti, F. and Della Ragione, F., 2020. Effects of Germline VHL Deficiency on Growth, Metabolism, and Mitochondria.  New England Journal of Medicine, 382(9), pp.835-844.

Hau, H., Ogundele, O., Hibbert, A., Monfries, C., Exelby, K., Wood, N., Nevarez-Mejia, J., Carbajal, M., Fleck, R., Dermit, M., Mardakheh, F., Williams-Ward, V., Pipalia, T., Conte, M. and Hughes, S., 2020. Maternal Larp6 controls oocyte development, chorion formation and elevation. Development

Petrova, A., Georgiadis, C., Fleck, R., Allison, L., McGrath, J., Dazzi, F., Di, W. and Qasim, W., 2020. Human Mesenchymal Stromal Cells Engineered to Express Collagen VII Can Restore Anchoring Fibrils in Recessive Dystrophic Epidermolysis Bullosa Skin Graft Chimeras. Journal of Investigative Dermatology, 140(1), pp.121-131.e6.

2019

Veraitch, O., Allison, L., Vizcay‐Barrena, G., Fleck, R., Price, A., Fenton, D., McGrath, J. and Stefanato, C., 2019. Detailed hair shaft analysis in a man with delayed‐onset Chediak‐Higashi syndrome. British Journal of Dermatology.

2017

So, E., Mitchell, J., Memmi, C., Chennell, G., Vizcay-Barrena, G., Allison, L., Shaw, C. and Vance, C., 2017. Mitochondrial abnormalities and disruption of the neuromuscular junction precede the clinical phenotype and motor neuron loss in hFUSWT transgenic mice. Human Molecular Genetics, 27(3), pp.463-474.

Jitraruch, S., Dhawan, A., Hughes, R., Filippi, C., Lehec, S., Glover, L. and Mitry, R., 2017. Cryopreservation of Hepatocyte Microbeads for Clinical TransplantationCell Transplantation, 26(8), pp.1341-1354.

Hale, V. L., Watermeyer, J. M., Hackett, F., Vizcay-Barrena, G., Van Ooij, C., Thomas, J. A., Spink, M. C., Harkiolaki, M., Duke, E., Fleck, R. A., Blackman, M. J. & Saibil, H. R. 28 Mar 2017 Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress In : Proceedings of the National Academy of Sciences of the United States of America. 114, 13, p. 3439-3444.

Walker, A. S., Neves, G., Grillo, F., Jackson, R. E., Rigby, M., O’Donnell, C., Lowe, A. S., Vizcay-Barrena, G., Fleck, R. A. & Burrone, J. 7 Mar 2017 Distance-dependent gradient in NMDAR-driven spine calcium signals along tapering dendritesIn : Proceedings of the National Academy of Sciences of the United States of America. 114, 10, p. E1986-E1995.

Sekhar, G. N., Georgian, A. R., Sanderson, L., Vizcay-Barrena, G., Brown, R. C., Muresan, P., Fleck, R. A. & Thomas, S. A.1 Mar 2017 Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB) In : PL o S One . 12, 3, e0173474.

2016

Liakath-Ali, K., Vancollie, V., Lelliott, C., Speak, A., Lafont, D., Protheroe, H., Ingvorsen, C., Galli, A., Green, A., Gleeson, D., Ryder, E., Glover, L., Vizcay-Barrena, G., Karp, N., Arends, M., Brenn, T., Spiegel, S., Adams, D., Watt, F. and van der Weyden, L., 2016. Alkaline ceramidase 1 is essential for mammalian skin homeostasis and regulating whole-body energy expenditure. The Journal of Pathology, 239(3), pp.374-383.

Lussignol, M., Kopp, M., Molloy, K., Vizcay-Barrena, G., Fleck, R. A., Dorner, M., Bell, K. L., Chait, B. T., Rice, C. M. & Catanese, M. T. 1 Mar 2016. Proteomics of HCV virions reveals an essential role for the nucleoporin Nup98 in virus morphogenesis In : Proceedings of the National Academy of Sciences of the United States of America. 113, 9, p. 2484-2489.

Chapple, S. J., Keeley, T. P., Mastronicola, D., Arno, M., Vizcay-Barrena, G., Fleck, R., Siow, R. & Mann, G. Mar 2016. Bach1 differentially regulates distinct Nrf2-dependent genes in human venous and coronary artery endothelial cells adapted to physiological oxygen levels In : Free radical biology & medicine. 92, p. 152-162.

Veraitch, O., Perez, A., Hoque, S. R., Vizcay-Barrena, G., Fleck, R. A., Fenton, D. A. & Stefanato, C. M. Mar 2016. Hair Follicle Miniaturization in a Woolly Hair Nevus: A Novel “Root” Perspective for a Mosaic Hair Disorder In : American Journal of Dermatopathology. 38, 3, p. 239-243.

Wbp2 is required for normal glutamatergic synapses in the cochlea and is crucial for hearingBuniello, A., Ingham, N. J., Lewis, M. A., Huma, A. C., Martinez-Vega, R., Varela-Nieto, I., Vizcay-Barrena, G., Fleck, R. A., Houston, O., Bardhan, T., Johnson, S. L., White, J. K., Yuan, H., Marcotti, W. & Steel, K. P. 8 Feb 2016 In : EMBO Molecular Medicine. 8, 3, p. 191.-207.

Green, M., Haigh, S. J., Lewis, E. A., Sandiford, L., Burkitt-Gray, M., Fleck, R., Vizcay-Barrena, G., Jensen, L., Mirzai, H., Curry, R. J. & Dailey, L. A. 9 Feb 2016 The Biosynthesis of Infrared-Emitting Quantum Dots in Allium Fistulosum In : Scientific Reports. 6, 20480.

Georgiadis, C., Syed, F., Petrova, A., Abdul-Wahab, A., Lwin, S., Farzaneh, F., Chan, L., Ghani, S., Fleck, R., Glover, L., McMillan, J., Chen, M., Thrasher, A., McGrath, J., Di, W. and Qasim, W., 2016. Lentiviral Engineered Fibroblasts Expressing Codon-Optimized COL7A1 Restore Anchoring Fibrils in RDEB. Journal of Investigative Dermatology, 136(1), pp.284-292.

Watermeyer, J. M., Hale, V. L., Hackett, F., Clare, D. K., Cutts, E. E., Vakonakis, I., Fleck, R., Blackman, M. J. & Saibil, H. R. 21 Jan 2016.  A spiral scaffold underlies cytoadherent knobs in Plasmodium falciparum-infected erythrocytes In : Blood. 127, 3, p. 343-351.

2015

Mitchell, J., Constable, R., So, E., Vance, C., Scotter, E., Glover, L., Hortobagyi, T., Arnold, E., Ling, S., McAlonis, M., Da Cruz, S., Polymenidou, M., Tessarolo, L., Cleveland, D. and Shaw, C., 2015. Wild type human TDP-43 potentiates ALS-linked mutant TDP-43 driven progressive motor and cortical neuron degeneration with pathological features of ALS. Acta Neuropathologica Communications, 3(1).

Fleck, R.A. (2015) Low temperature electron microscopy, in, Methods in Cryopreservation and Freeze-Drying (edsW.Wolkers and H. Oldenhof), Lab Protocol Series, Methods in Molecular Biology, Springer, Berlin, pp. 243–274.

2013

Carlin LM, Stamatiades EG, Auffray C, Hanna RN, Glover L, Vizcay-Barrena G, Hedrick CC, Cook HT, Diebold S, Geissmann F. Cell. 2013 Apr. Nr4a1-dependent Ly6C(low) monocytes monitor endothelial cells and orchestrate their disposal. 11;153(2):362-75.

Different types of access to the Centre are available. We are a collaborative facility and support researchers from experimental design to data interpretation and preparation for publication. We offer electron microscopy services and support and provide training so that researchers can use CUI instruments independently.

If you are interested in working with us, please get in touch. We aim to respond to enquiries within 5 working days.

After an initial discussion about your project, you will be asked to complete a project application form, providing a brief description of the work you wish to carry out and what you expect to achieve using electron microscopy (EM). This will help the team select the best techniques for your project.

To ensure we give the best help and support to research, King’s CUI Strategic Advisory Committee help the CUI team review and prioritise projects.

Training

Training on instruments is provided by CUI staff. All new uses need to complete training before unsupervised use of the instruments is granted. The level of training and the time required will vary depending on the instrument.

Booking equipment

Once a new user has completed training, time on microscopes can be booked by contacting the Centre. Sessions are normally three hours 9.30-12.30hrs and 13.30-16.30hrs. Independent users can book sessions via the booking system.

Cancelling a booking

Users can cancel a booking by contacting the Centre. Please give 24 hours’ notice. If users are more than 30 minutes late for a booking, they should contact the Centre, or the session will be cancelled, and instrument time will be charged as normal.

Publication and acknowledgement

Where a significant contribution has been identified, researchers are expected to acknowledge the contribution of CUI staff by way of authorship, in accordance with the Core Facilities Fair Publication Policy.

All images for publication purposes used should be reviewed and approved between CUI staff and researchers to ensure consistent high standards.

Publications that include data generated in the Centre should be shared with the CUI team.

We provide cutting-edge and comprehensive workflows to support life and materials science electron microscopy.  Electron microscopes come in a variety of shapes and sizes and when used correctly, they are powerful investigative tools, capable of revealing a fascinating miniature world which is not visible with the naked eye, or even with a light microscope.

We work with leading international research groups, as well as instrument manufacturers to support advanced electron microscopy techniques and promote novel ideas and innovation.  

Here are some of the techniques available in the CUI.

Facility staff

Roland

Director of Centre for Ultrastructural Imaging

Leanne

Facility Manager - Centre for Ultrastructural Imaging

James Gereats

Cryo-Electron Microscopy Application Specialist

Matt

Electron Microscopy Applications Specialist

Ale

Electron Microscopy Technician

Shannon Stockdale2

Electron Microscopy Technician

Equipment available at the CUI

Related equipment

JEOL 1400Plus FLASH
JEOL JEM-1400Flash

The JEOL JEM-1400Flash is used to study a variety of materials from nanoparticles, viruses, bacteria, and sections of cells and tissues.

F200 picture
JEOL JEM-F200 TEM

The JEOL JEM-F200 is our current cryo-TEM and is used for screening of cryo-samples as well as room temperature applications such as tomography.

7800 Prime b
JEOL JSM 7800F Prime Cryo-SEM

The JEOL JSM 7800F Prime SEM is our current top end SEM, equipped with a Schottky FEG and has the capability to run in cryo mode.

JSM-7800 a
JEOL JSM 7800F SBF-SEM

The JSM 7800F Serial Block Face SEM is the same microscope as our top end JSM 7800F Prime Cryo-SEM and it is equipped with a katana microtome for volume EM.

4. Scanning Transmission Electron Microscopy- High Angle Annular Darkfield Imaging (STEM-HAADF)
JEOL JIB-4700F Z Dual Beam FIB-SEM

The microscope is used to create lamellae for electron tomography and volume electron microscopy

JEOL JCM7000 NEOSCOPE
JEOL JCM 7000 NeoScope Benchtop SEM

The JEOL JCM 7000 NeoScope is our smallest electron microscope, but despite its small size it is a very capable instrument packed with features.

2023

Burkitt Grey, M., Fleck, RA., Casavola, M., Clark, P., Fairclough, S., Flavell, W., Haigh, S., Chun-Ren Ke, J., Leontiadou, M., Lewis, E., Osiecki, J., Qazi-Chaudhry, B., Vizcay-Barrena, G., Wichiansee, W., Green., M. (2023), Structural investigations into colour-tuneable fluorescent InZnP-based quantum dots from zinc carboxylate and aminophosphine precursors, Nanoscale.2023.10.1039.

Petrova, A., Allison, L., Fleck, RA., Naso, G., Athina Gkazi, S., Georgiadis, C., Jayarajan, V., Jacków, J., Kayode Ogunbiyi, O., McGrath, JA., Ilic, D., Di, W., Qasim, W. (2023), Cytosine Deaminase Base Editing to Restore COL7A1in Dystrophic Epidermolysis Bullosa Human: Murine Skin Model, JID Innovations.2023.10.1016

Morfill, C., Fleck, RA., Pankratova, S., Machado, P., Fernado, NK., Regoutz, A., Talamona, F., Pinna, A., Klosowski, M., Wilkinson, R., Xie, Fang., Porter, A., Kiryushko, D. (2023), Addition to "Nanostars Carrying Multifunctional Neurotrophic Dendrimers Protect Neurons in Preclinical In Vitro Models of Neurodegenerative Disorders", ACS Applied Materials & Interfaces.2023.10.1021.

Rigby, M., Fleck, RA., Grillo, F., Compans, B., Neves, G., Gallinaro, J., Nashashibi, S., Horton, S., Pereira Machado, P., Carbajal, MA., Vizcay-Barrena, G., Levet, F., Sibarita, JB., Kirkland, A., Clopath, C., Burrone, J. (2023), Multi-synaptic boutons are a feature of CA1 hippocampal connections in the stratum oriens, Cell Reports.2023.10.1016.

Bille, A., Allison, L., Fleck, RA., Russell, M., Jhala, H., Ashrafian, L., Nonaka, D. (2023), Does hyperthermic povidone-iodine lavage increase the apoptotic rate of residual cancer cell in patients with malignant pleural mesothelioma? -A prospective pilot study, Translational Lung Cancer Research.2023.10.21037.

Kobylynska, M., Fleck, RA. Nicholls, D., Wells, J., Robinson, A., Moshtaghpour, A., Kobylynska, M., Kirkland, A., Mehdi, L., Browning, N. (2023), Compressive Cryo FIB-SEM Tomography, Microscopy and Microanalysis. Microscopy and Microanalysis.2023.0.1093

Zhang, W., Fleck, RA., Carbajal, AM., Metzger, H., Vlatakis, S., Claxton, A., Fung, LF., Mason, J., Chan, KL.A., Pouliopoulos, A., Prentice, P., Thanou, M. (2023), Characterising the chemical and physical properties of phase-change nanodroplets, Ultrasonics Sonochemistry.2023.10.10.16.

2022

Morotz M., Fleck, RA., Russell, M., Martín-Guerrero, S., Markovinovic, A., Paillusson, S., Pereira Machado, P., Noble, W., Miller, C. (2022), The PTPIP51 coiled-coil domain is important in VAPB binding, formation of ER-mitochondria contacts and IP3 receptor delivery of Ca2+ to mitochondria, Frontiers in cell and developmental biology, Frontiers in Cell.2022.10.3389.

Bernard, C., Allison, L., Fleck, RA., Selten, J., Hanusz-Godoy, A., Aguilera, A., Hamid, F., Maeso, P., Russell, M., Oozeer, F., Sanalidou, S., Exposito-Alonso, D., Rico, B., Marín, O. (2022), Cortical wiring by synapse type–specific control of local protein synthesis, Science.2022.0.1126. 

2021

Wallis, S., Ventimiglia, L., Otigbah, E., Infante, E., Cuesta-Geijo, M., Kidiyoor, G., Carbajal, M., Fleck, R., Foiani, M., Garcia-Manyes, S., Martin-Serrano, J. and Agromayor, M., 2021. The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.  Developmental Cell, 56(23), pp.3192-3202.e8.

2020

Matsubayashi, Y., Sánchez-Sánchez, B., Marcotti, S., Serna-Morales, E., Dragu, A., Díaz-de-la-Loza, M., Vizcay-Barrena, G., Fleck, R. and Stramer, B., 2020. Rapid Homeostatic Turnover of Embryonic ECM during Tissue Morphogenesis. Developmental Cell, 54(1), pp.33-42.e9.

Valente, P., Kiryushko, D., Sacchetti, S., Machado, P., Cobley, C., Mangini, V., Porter, A., Spatz, J., Fleck, R., Benfenati, F. and Fiammengo, R., 2020. Conopeptide-Functionalized Nanoparticles Selectively Antagonize ExtrasynapticN-Methyl-d-aspartate Receptors and Protect Hippocampal Neurons from Excitotoxicity In Vitro. ACS Nano, 14(6), pp.6866-6877.

Awan, M., Buriak, I., Fleck, R., Fuller, B., Goltsev, A., Kerby, J., Lowdell, M., Mericka, P., Petrenko, A., Petrenko, Y., Rogulska, O., Stolzing, A. and Stacey, G., 2020 Dimethyl sulfoxide: a central player since the dawn of cryobiology, is efficacy balanced by toxicity? Regenerative Medicine, 15(3), pp.1463-1491.

Perrotta, S., Roberti, D., Bencivenga, D., Corsetto, P., O’Brien, K., Caiazza, M., Stampone, E., Allison, L., Fleck, R., Scianguetta, S., Tartaglione, I., Robbins, P., Casale, M., West, J., Franzini-Armstrong, C., Griffin, J., Rizzo, A., Sinisi, A., Murray, A., Borriello, A., Formenti, F. and Della Ragione, F., 2020. Effects of Germline VHL Deficiency on Growth, Metabolism, and Mitochondria.  New England Journal of Medicine, 382(9), pp.835-844.

Hau, H., Ogundele, O., Hibbert, A., Monfries, C., Exelby, K., Wood, N., Nevarez-Mejia, J., Carbajal, M., Fleck, R., Dermit, M., Mardakheh, F., Williams-Ward, V., Pipalia, T., Conte, M. and Hughes, S., 2020. Maternal Larp6 controls oocyte development, chorion formation and elevation. Development

Petrova, A., Georgiadis, C., Fleck, R., Allison, L., McGrath, J., Dazzi, F., Di, W. and Qasim, W., 2020. Human Mesenchymal Stromal Cells Engineered to Express Collagen VII Can Restore Anchoring Fibrils in Recessive Dystrophic Epidermolysis Bullosa Skin Graft Chimeras. Journal of Investigative Dermatology, 140(1), pp.121-131.e6.

2019

Veraitch, O., Allison, L., Vizcay‐Barrena, G., Fleck, R., Price, A., Fenton, D., McGrath, J. and Stefanato, C., 2019. Detailed hair shaft analysis in a man with delayed‐onset Chediak‐Higashi syndrome. British Journal of Dermatology.

2017

So, E., Mitchell, J., Memmi, C., Chennell, G., Vizcay-Barrena, G., Allison, L., Shaw, C. and Vance, C., 2017. Mitochondrial abnormalities and disruption of the neuromuscular junction precede the clinical phenotype and motor neuron loss in hFUSWT transgenic mice. Human Molecular Genetics, 27(3), pp.463-474.

Jitraruch, S., Dhawan, A., Hughes, R., Filippi, C., Lehec, S., Glover, L. and Mitry, R., 2017. Cryopreservation of Hepatocyte Microbeads for Clinical TransplantationCell Transplantation, 26(8), pp.1341-1354.

Hale, V. L., Watermeyer, J. M., Hackett, F., Vizcay-Barrena, G., Van Ooij, C., Thomas, J. A., Spink, M. C., Harkiolaki, M., Duke, E., Fleck, R. A., Blackman, M. J. & Saibil, H. R. 28 Mar 2017 Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress In : Proceedings of the National Academy of Sciences of the United States of America. 114, 13, p. 3439-3444.

Walker, A. S., Neves, G., Grillo, F., Jackson, R. E., Rigby, M., O’Donnell, C., Lowe, A. S., Vizcay-Barrena, G., Fleck, R. A. & Burrone, J. 7 Mar 2017 Distance-dependent gradient in NMDAR-driven spine calcium signals along tapering dendritesIn : Proceedings of the National Academy of Sciences of the United States of America. 114, 10, p. E1986-E1995.

Sekhar, G. N., Georgian, A. R., Sanderson, L., Vizcay-Barrena, G., Brown, R. C., Muresan, P., Fleck, R. A. & Thomas, S. A.1 Mar 2017 Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB) In : PL o S One . 12, 3, e0173474.

2016

Liakath-Ali, K., Vancollie, V., Lelliott, C., Speak, A., Lafont, D., Protheroe, H., Ingvorsen, C., Galli, A., Green, A., Gleeson, D., Ryder, E., Glover, L., Vizcay-Barrena, G., Karp, N., Arends, M., Brenn, T., Spiegel, S., Adams, D., Watt, F. and van der Weyden, L., 2016. Alkaline ceramidase 1 is essential for mammalian skin homeostasis and regulating whole-body energy expenditure. The Journal of Pathology, 239(3), pp.374-383.

Lussignol, M., Kopp, M., Molloy, K., Vizcay-Barrena, G., Fleck, R. A., Dorner, M., Bell, K. L., Chait, B. T., Rice, C. M. & Catanese, M. T. 1 Mar 2016. Proteomics of HCV virions reveals an essential role for the nucleoporin Nup98 in virus morphogenesis In : Proceedings of the National Academy of Sciences of the United States of America. 113, 9, p. 2484-2489.

Chapple, S. J., Keeley, T. P., Mastronicola, D., Arno, M., Vizcay-Barrena, G., Fleck, R., Siow, R. & Mann, G. Mar 2016. Bach1 differentially regulates distinct Nrf2-dependent genes in human venous and coronary artery endothelial cells adapted to physiological oxygen levels In : Free radical biology & medicine. 92, p. 152-162.

Veraitch, O., Perez, A., Hoque, S. R., Vizcay-Barrena, G., Fleck, R. A., Fenton, D. A. & Stefanato, C. M. Mar 2016. Hair Follicle Miniaturization in a Woolly Hair Nevus: A Novel “Root” Perspective for a Mosaic Hair Disorder In : American Journal of Dermatopathology. 38, 3, p. 239-243.

Wbp2 is required for normal glutamatergic synapses in the cochlea and is crucial for hearingBuniello, A., Ingham, N. J., Lewis, M. A., Huma, A. C., Martinez-Vega, R., Varela-Nieto, I., Vizcay-Barrena, G., Fleck, R. A., Houston, O., Bardhan, T., Johnson, S. L., White, J. K., Yuan, H., Marcotti, W. & Steel, K. P. 8 Feb 2016 In : EMBO Molecular Medicine. 8, 3, p. 191.-207.

Green, M., Haigh, S. J., Lewis, E. A., Sandiford, L., Burkitt-Gray, M., Fleck, R., Vizcay-Barrena, G., Jensen, L., Mirzai, H., Curry, R. J. & Dailey, L. A. 9 Feb 2016 The Biosynthesis of Infrared-Emitting Quantum Dots in Allium Fistulosum In : Scientific Reports. 6, 20480.

Georgiadis, C., Syed, F., Petrova, A., Abdul-Wahab, A., Lwin, S., Farzaneh, F., Chan, L., Ghani, S., Fleck, R., Glover, L., McMillan, J., Chen, M., Thrasher, A., McGrath, J., Di, W. and Qasim, W., 2016. Lentiviral Engineered Fibroblasts Expressing Codon-Optimized COL7A1 Restore Anchoring Fibrils in RDEB. Journal of Investigative Dermatology, 136(1), pp.284-292.

Watermeyer, J. M., Hale, V. L., Hackett, F., Clare, D. K., Cutts, E. E., Vakonakis, I., Fleck, R., Blackman, M. J. & Saibil, H. R. 21 Jan 2016.  A spiral scaffold underlies cytoadherent knobs in Plasmodium falciparum-infected erythrocytes In : Blood. 127, 3, p. 343-351.

2015

Mitchell, J., Constable, R., So, E., Vance, C., Scotter, E., Glover, L., Hortobagyi, T., Arnold, E., Ling, S., McAlonis, M., Da Cruz, S., Polymenidou, M., Tessarolo, L., Cleveland, D. and Shaw, C., 2015. Wild type human TDP-43 potentiates ALS-linked mutant TDP-43 driven progressive motor and cortical neuron degeneration with pathological features of ALS. Acta Neuropathologica Communications, 3(1).

Fleck, R.A. (2015) Low temperature electron microscopy, in, Methods in Cryopreservation and Freeze-Drying (edsW.Wolkers and H. Oldenhof), Lab Protocol Series, Methods in Molecular Biology, Springer, Berlin, pp. 243–274.

2013

Carlin LM, Stamatiades EG, Auffray C, Hanna RN, Glover L, Vizcay-Barrena G, Hedrick CC, Cook HT, Diebold S, Geissmann F. Cell. 2013 Apr. Nr4a1-dependent Ly6C(low) monocytes monitor endothelial cells and orchestrate their disposal. 11;153(2):362-75.

Different types of access to the Centre are available. We are a collaborative facility and support researchers from experimental design to data interpretation and preparation for publication. We offer electron microscopy services and support and provide training so that researchers can use CUI instruments independently.

If you are interested in working with us, please get in touch. We aim to respond to enquiries within 5 working days.

After an initial discussion about your project, you will be asked to complete a project application form, providing a brief description of the work you wish to carry out and what you expect to achieve using electron microscopy (EM). This will help the team select the best techniques for your project.

To ensure we give the best help and support to research, King’s CUI Strategic Advisory Committee help the CUI team review and prioritise projects.

Training

Training on instruments is provided by CUI staff. All new uses need to complete training before unsupervised use of the instruments is granted. The level of training and the time required will vary depending on the instrument.

Booking equipment

Once a new user has completed training, time on microscopes can be booked by contacting the Centre. Sessions are normally three hours 9.30-12.30hrs and 13.30-16.30hrs. Independent users can book sessions via the booking system.

Cancelling a booking

Users can cancel a booking by contacting the Centre. Please give 24 hours’ notice. If users are more than 30 minutes late for a booking, they should contact the Centre, or the session will be cancelled, and instrument time will be charged as normal.

Publication and acknowledgement

Where a significant contribution has been identified, researchers are expected to acknowledge the contribution of CUI staff by way of authorship, in accordance with the Core Facilities Fair Publication Policy.

All images for publication purposes used should be reviewed and approved between CUI staff and researchers to ensure consistent high standards.

Publications that include data generated in the Centre should be shared with the CUI team.


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